Lutimaribacter saemankumensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Lutimaribacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8LEF2|A0A1G8LEF2_9RHOB DNA ligase (ATP) OS=Lutimaribacter saemankumensis OX=490829 GN=SAMN05421850_103264 PE=4 SV=1
MM1 pKa = 7.32FNKK4 pKa = 9.94IKK6 pKa = 10.32PVLATAILAGVAACAQAPEE25 pKa = 4.31PVGVAPTFDD34 pKa = 3.55KK35 pKa = 11.12LGNAYY40 pKa = 10.45CPVGYY45 pKa = 10.37ALSGDD50 pKa = 3.61VCIPAKK56 pKa = 9.61SASMSDD62 pKa = 3.84DD63 pKa = 3.16GHH65 pKa = 6.14GTTASTDD72 pKa = 3.08SGTDD76 pKa = 3.38DD77 pKa = 4.83SSDD80 pKa = 3.31TGTDD84 pKa = 3.12TDD86 pKa = 3.98TDD88 pKa = 4.02TPGDD92 pKa = 4.12DD93 pKa = 4.5DD94 pKa = 4.82NGGGGNQNQSQNSNQNSNQNSNQNSNQNSNQNSNQSGNPNDD135 pKa = 3.6

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8RF25|A0A1G8RF25_9RHOB Putative ABC transport system ATP-binding protein OS=Lutimaribacter saemankumensis OX=490829 GN=SAMN05421850_10993 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MASKK25 pKa = 10.56AGRR28 pKa = 11.84KK29 pKa = 8.71ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3753

0

3753

1133726

25

1972

302.1

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.381 ± 0.051

0.889 ± 0.012

6.265 ± 0.04

5.81 ± 0.037

3.727 ± 0.024

8.881 ± 0.044

2.107 ± 0.02

5.162 ± 0.029

3.124 ± 0.029

9.911 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.021

2.512 ± 0.019

5.166 ± 0.027

3.173 ± 0.021

6.907 ± 0.036

4.812 ± 0.027

5.276 ± 0.023

7.315 ± 0.03

1.459 ± 0.016

2.214 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski