Canis familiaris polyomavirus 1
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6EU85|A0A1W6EU85_9POLY Large T antigen OS=Canis familiaris polyomavirus 1 OX=1980633 PE=4 SV=1
MM1 pKa = 6.71 FQYY4 pKa = 10.79 YY5 pKa = 10.27 NKK7 pKa = 10.38 LVTILLEE14 pKa = 4.73 LIDD17 pKa = 4.82 VIEE20 pKa = 5.41 LMITLQGLRR29 pKa = 11.84 TVRR32 pKa = 11.84 EE33 pKa = 4.11 VPEE36 pKa = 4.5 DD37 pKa = 3.59 CCDD40 pKa = 3.44 NARR43 pKa = 11.84 KK44 pKa = 9.61 VWGAA48 pKa = 3.18
Molecular weight: 5.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.747
IPC2_protein 4.965
IPC_protein 4.622
Toseland 4.469
ProMoST 4.724
Dawson 4.558
Bjellqvist 4.711
Wikipedia 4.444
Rodwell 4.457
Grimsley 4.38
Solomon 4.546
Lehninger 4.507
Nozaki 4.685
DTASelect 4.8
Thurlkill 4.482
EMBOSS 4.457
Sillero 4.724
Patrickios 3.719
IPC_peptide 4.558
IPC2_peptide 4.711
IPC2.peptide.svr19 4.749
Protein with the highest isoelectric point:
>tr|A0A1W6EU81|A0A1W6EU81_9POLY Small t antigen OS=Canis familiaris polyomavirus 1 OX=1980633 PE=4 SV=1
MM1 pKa = 7.64 ALVQWVPEE9 pKa = 3.97 QVDD12 pKa = 3.28 ILFPGVNWLVRR23 pKa = 11.84 NIHH26 pKa = 5.75 YY27 pKa = 8.13 FNPAIWAQEE36 pKa = 3.76 AFRR39 pKa = 11.84 VVWQQIQRR47 pKa = 11.84 EE48 pKa = 4.43 TVRR51 pKa = 11.84 QIAYY55 pKa = 6.69 EE56 pKa = 3.96 ANRR59 pKa = 11.84 AVQEE63 pKa = 4.09 RR64 pKa = 11.84 GQEE67 pKa = 3.95 VGQQLGQLAVDD78 pKa = 4.9 GIARR82 pKa = 11.84 VIEE85 pKa = 4.02 NGQWVLSTTPEE96 pKa = 4.34 RR97 pKa = 11.84 IASGVSSAYY106 pKa = 10.0 QGLASYY112 pKa = 10.24 YY113 pKa = 10.67 GQLTHH118 pKa = 6.91 IRR120 pKa = 11.84 PSQQSQVARR129 pKa = 11.84 SLQVGQQAGPISFTTPQSGEE149 pKa = 3.96 FIKK152 pKa = 10.79 QYY154 pKa = 10.53 DD155 pKa = 3.91 SPGGAEE161 pKa = 4.72 QRR163 pKa = 11.84 RR164 pKa = 11.84 CPNWLLPLILGFYY177 pKa = 7.45 NTPVWTAAKK186 pKa = 10.12 KK187 pKa = 10.2 KK188 pKa = 10.7 VEE190 pKa = 3.94 EE191 pKa = 4.21 TKK193 pKa = 10.64 PKK195 pKa = 9.92 NVYY198 pKa = 9.99 RR199 pKa = 11.84 KK200 pKa = 8.77 RR201 pKa = 11.84 KK202 pKa = 9.33 SSASSKK208 pKa = 9.09 TNYY211 pKa = 8.81 KK212 pKa = 9.72 RR213 pKa = 11.84 RR214 pKa = 11.84 RR215 pKa = 11.84 ISTRR219 pKa = 11.84 VKK221 pKa = 10.5 NRR223 pKa = 11.84 AA224 pKa = 3.08
Molecular weight: 25.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.721
IPC_protein 10.321
Toseland 10.365
ProMoST 10.087
Dawson 10.54
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 10.833
Grimsley 10.613
Solomon 10.584
Lehninger 10.555
Nozaki 10.335
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.452
Patrickios 10.482
IPC_peptide 10.584
IPC2_peptide 9.048
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1847
48
707
307.8
34.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.956 ± 1.519
2.653 ± 0.802
4.115 ± 0.889
7.58 ± 1.231
3.573 ± 0.629
7.093 ± 1.09
1.408 ± 0.239
4.981 ± 0.504
6.605 ± 1.371
9.15 ± 0.775
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.382 ± 0.475
5.793 ± 0.717
4.169 ± 0.74
6.064 ± 0.832
4.656 ± 0.787
5.739 ± 0.948
5.793 ± 0.835
7.093 ± 0.776
2.112 ± 0.539
3.086 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here