Hubei toti-like virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFG5|A0A1L3KFG5_9VIRU Uncharacterized protein OS=Hubei toti-like virus 17 OX=1923305 PE=4 SV=1
MM1 pKa = 7.55SGLAPPSSDD10 pKa = 3.11TPASLPEE17 pKa = 3.93AAGATGSGTAQAPVPATSRR36 pKa = 11.84QAPGGASEE44 pKa = 4.18AQGAKK49 pKa = 10.07VPAVSPLTPHH59 pKa = 7.46DD60 pKa = 3.98EE61 pKa = 4.21QSSSEE66 pKa = 4.14RR67 pKa = 11.84TVGPMGKK74 pKa = 9.65ALAYY78 pKa = 7.46VTQEE82 pKa = 3.85ATRR85 pKa = 11.84LPALYY90 pKa = 8.55GTGDD94 pKa = 3.69DD95 pKa = 3.91TRR97 pKa = 11.84SLAEE101 pKa = 3.32WWLAQRR107 pKa = 11.84GGYY110 pKa = 9.02AAIGDD115 pKa = 4.25SSSVYY120 pKa = 10.57AGYY123 pKa = 9.57QAYY126 pKa = 10.4LGLSVEE132 pKa = 4.63VYY134 pKa = 10.1NYY136 pKa = 10.17TGVRR140 pKa = 11.84SQIVDD145 pKa = 5.03PIRR148 pKa = 11.84QWGGWYY154 pKa = 7.86WRR156 pKa = 11.84AVEE159 pKa = 4.16GVDD162 pKa = 3.83VGGEE166 pKa = 4.11VFAYY170 pKa = 8.36PANLEE175 pKa = 4.29SAISRR180 pKa = 11.84ATTGDD185 pKa = 3.35ITPLSQLLRR194 pKa = 11.84IKK196 pKa = 10.22SWALEE201 pKa = 3.94GTTLVMMSRR210 pKa = 11.84MLATSLSANPGNSTAVILARR230 pKa = 11.84LLGYY234 pKa = 10.52LCALLVPADD243 pKa = 3.79PGGNVPASMPANSTVVAEE261 pKa = 4.69GQWFPYY267 pKa = 10.39HH268 pKa = 7.4PDD270 pKa = 3.68PGDD273 pKa = 3.26AMGRR277 pKa = 11.84IPCIYY282 pKa = 7.22TTLEE286 pKa = 3.83VFVRR290 pKa = 11.84WTLGEE295 pKa = 3.85QNIPGWEE302 pKa = 4.11ALEE305 pKa = 3.99MDD307 pKa = 4.16RR308 pKa = 11.84SIVVVPVDD316 pKa = 4.7HH317 pKa = 7.05EE318 pKa = 4.24ATLNPRR324 pKa = 11.84AAAWMTVGMMEE335 pKa = 4.11YY336 pKa = 9.94PVRR339 pKa = 11.84RR340 pKa = 11.84LIRR343 pKa = 11.84MGAPRR348 pKa = 11.84DD349 pKa = 3.46PLGPLRR355 pKa = 11.84TRR357 pKa = 11.84QNAGGWLGQWIPSQMLMRR375 pKa = 11.84IDD377 pKa = 3.43GRR379 pKa = 11.84GGVSADD385 pKa = 3.06ATRR388 pKa = 11.84VMFVRR393 pKa = 11.84VSGSMGVGTPALMLPGIANGLTPFIGVGQPDD424 pKa = 3.58EE425 pKa = 5.43RR426 pKa = 11.84DD427 pKa = 3.38FTMTLASLFLNPSAQVPYY445 pKa = 9.96IYY447 pKa = 10.26EE448 pKa = 4.06AFLRR452 pKa = 11.84TVSVIGAGGAYY463 pKa = 9.41GVALRR468 pKa = 11.84FWGQHH473 pKa = 4.92GILWPRR479 pKa = 11.84PWEE482 pKa = 4.0VWGTGPPADD491 pKa = 4.27KK492 pKa = 10.12STTGLAPAHH501 pKa = 5.35TVDD504 pKa = 4.86RR505 pKa = 11.84GWLPRR510 pKa = 11.84EE511 pKa = 4.34AYY513 pKa = 10.29SAFTPEE519 pKa = 4.7RR520 pKa = 11.84ALSDD524 pKa = 3.74ANWFVNSAGMLPGVLYY540 pKa = 10.68GGHH543 pKa = 6.0NRR545 pKa = 11.84QGALSGAAVHH555 pKa = 6.12FTLPRR560 pKa = 11.84YY561 pKa = 10.17SSVFGIAAAAGVAILTPGQEE581 pKa = 4.33SLKK584 pKa = 10.07GLSPIALVAGVMRR597 pKa = 11.84GSLQYY602 pKa = 11.29ACLYY606 pKa = 10.7DD607 pKa = 4.07LLFAVRR613 pKa = 11.84GVARR617 pKa = 11.84EE618 pKa = 3.98EE619 pKa = 4.15IILNYY624 pKa = 9.89RR625 pKa = 11.84DD626 pKa = 4.1AFPATKK632 pKa = 9.21ATRR635 pKa = 11.84FRR637 pKa = 11.84EE638 pKa = 3.56RR639 pKa = 11.84WGYY642 pKa = 10.53QGVSQPGGGSMAEE655 pKa = 3.53IAYY658 pKa = 9.69IGINQRR664 pKa = 11.84ALRR667 pKa = 11.84CYY669 pKa = 9.56WPTNGQVFHH678 pKa = 6.86TPNADD683 pKa = 3.36DD684 pKa = 4.13VGAGMHH690 pKa = 5.83IFGRR694 pKa = 11.84IAAPVLRR701 pKa = 11.84AIVPDD706 pKa = 4.0LPVSRR711 pKa = 11.84ATFGTFSEE719 pKa = 4.92VFGSLQLPNAEE730 pKa = 3.63RR731 pKa = 11.84TGIEE735 pKa = 4.02EE736 pKa = 4.29GFLTVGAADD745 pKa = 3.45DD746 pKa = 3.81WDD748 pKa = 4.31RR749 pKa = 11.84AWKK752 pKa = 10.45LSALFSGLDD761 pKa = 3.41PFNNPEE767 pKa = 3.83MGDD770 pKa = 3.22RR771 pKa = 11.84FQIFMRR777 pKa = 11.84SCPDD781 pKa = 2.95DD782 pKa = 3.56TSPYY786 pKa = 7.58TCAVVGPLARR796 pKa = 11.84FAALAIGDD804 pKa = 4.16FATASSRR811 pKa = 11.84YY812 pKa = 8.77SWLKK816 pKa = 7.94RR817 pKa = 11.84TWWLTIDD824 pKa = 4.42RR825 pKa = 11.84PPPAIDD831 pKa = 2.93WNLAFGLGIRR841 pKa = 11.84CTDD844 pKa = 2.98RR845 pKa = 11.84TLALSSRR852 pKa = 11.84AFEE855 pKa = 4.14FKK857 pKa = 10.32RR858 pKa = 11.84WDD860 pKa = 3.7FGEE863 pKa = 4.6GAVSAPDD870 pKa = 3.63TNVVGDD876 pKa = 4.04ALGAMRR882 pKa = 11.84NASANPFF889 pKa = 3.53

Molecular weight: 95.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF43|A0A1L3KF43_9VIRU Uncharacterized protein OS=Hubei toti-like virus 17 OX=1923305 PE=4 SV=1
MM1 pKa = 7.45GRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84WRR7 pKa = 11.84VPNRR11 pKa = 11.84YY12 pKa = 8.61PSLGGRR18 pKa = 11.84WGTAFTAYY26 pKa = 10.0FAALPPAAKK35 pKa = 8.67KK36 pKa = 8.02TAEE39 pKa = 4.16DD40 pKa = 3.44WFQGLRR46 pKa = 11.84IQEE49 pKa = 4.11FRR51 pKa = 11.84DD52 pKa = 3.61DD53 pKa = 4.16RR54 pKa = 11.84SLCEE58 pKa = 3.83VLKK61 pKa = 10.76EE62 pKa = 4.02LGGFAKK68 pKa = 10.42KK69 pKa = 10.57YY70 pKa = 11.08GEE72 pKa = 5.07FVTNWKK78 pKa = 10.36VLVNWEE84 pKa = 4.18LFGGYY89 pKa = 7.67KK90 pKa = 8.43TQADD94 pKa = 3.77DD95 pKa = 3.71EE96 pKa = 4.48QFQEE100 pKa = 5.95DD101 pKa = 3.75VRR103 pKa = 11.84DD104 pKa = 3.76WLNGRR109 pKa = 11.84LPPHH113 pKa = 6.41EE114 pKa = 4.13WRR116 pKa = 11.84DD117 pKa = 3.29GSFYY121 pKa = 11.3DD122 pKa = 5.7LFNQGCLDD130 pKa = 4.88FLQFWKK136 pKa = 10.68APRR139 pKa = 11.84AEE141 pKa = 4.23PLPLPEE147 pKa = 4.23YY148 pKa = 10.34CGSLMWARR156 pKa = 11.84GGASSEE162 pKa = 4.06AGVEE166 pKa = 3.96VLEE169 pKa = 4.3EE170 pKa = 4.28GKK172 pKa = 10.5RR173 pKa = 11.84LAVRR177 pKa = 11.84GSKK180 pKa = 10.32VSSSLAIPPSKK191 pKa = 10.65VEE193 pKa = 3.91EE194 pKa = 4.93RR195 pKa = 11.84ILSYY199 pKa = 10.5TPQVSKK205 pKa = 10.92AIQKK209 pKa = 10.66LEE211 pKa = 4.02GGKK214 pKa = 9.73VRR216 pKa = 11.84AVVNSDD222 pKa = 2.98LDD224 pKa = 4.33LYY226 pKa = 11.62LKK228 pKa = 9.82MDD230 pKa = 3.93WLSTWVEE237 pKa = 3.95AGFKK241 pKa = 10.29GSPISTLFMTGPAVADD257 pKa = 4.16FWEE260 pKa = 4.9RR261 pKa = 11.84FASGTLDD268 pKa = 3.7EE269 pKa = 4.94RR270 pKa = 11.84WVKK273 pKa = 10.93APLDD277 pKa = 3.5QSRR280 pKa = 11.84FDD282 pKa = 3.67HH283 pKa = 6.65QPDD286 pKa = 3.55FRR288 pKa = 11.84MLGHH292 pKa = 6.31VLDD295 pKa = 3.98AVEE298 pKa = 4.67RR299 pKa = 11.84LLVTDD304 pKa = 3.16QQRR307 pKa = 11.84LVLSHH312 pKa = 6.06VRR314 pKa = 11.84RR315 pKa = 11.84AMLGGGTVDD324 pKa = 3.2VEE326 pKa = 4.54GARR329 pKa = 11.84FPITKK334 pKa = 9.96GIVSGWRR341 pKa = 11.84WTALIDD347 pKa = 3.13TWINHH352 pKa = 5.88AEE354 pKa = 4.21MYY356 pKa = 10.56AATHH360 pKa = 6.69YY361 pKa = 9.97IRR363 pKa = 11.84QVIPRR368 pKa = 11.84AHH370 pKa = 6.59FEE372 pKa = 4.21WVVQGDD378 pKa = 4.19DD379 pKa = 3.61VRR381 pKa = 11.84EE382 pKa = 4.02LALSEE387 pKa = 4.24EE388 pKa = 4.45DD389 pKa = 3.8VVCQVEE395 pKa = 4.29ALVQAGFVINPGKK408 pKa = 10.51FFISRR413 pKa = 11.84KK414 pKa = 8.38RR415 pKa = 11.84DD416 pKa = 3.07EE417 pKa = 4.13FLRR420 pKa = 11.84RR421 pKa = 11.84VSEE424 pKa = 4.04HH425 pKa = 5.85GRR427 pKa = 11.84VSGYY431 pKa = 8.27PARR434 pKa = 11.84AMLGILWRR442 pKa = 11.84NPIKK446 pKa = 10.72EE447 pKa = 4.12EE448 pKa = 4.06APAGDD453 pKa = 3.51VRR455 pKa = 11.84IRR457 pKa = 11.84EE458 pKa = 4.3CLSRR462 pKa = 11.84WTGLANRR469 pKa = 11.84GADD472 pKa = 3.32PGACFRR478 pKa = 11.84MFVADD483 pKa = 4.06AARR486 pKa = 11.84GSALPGTVVRR496 pKa = 11.84RR497 pKa = 11.84WAHH500 pKa = 4.21TPEE503 pKa = 3.86WFGGGGVVPYY513 pKa = 9.58TDD515 pKa = 3.35QPIQIKK521 pKa = 9.32VQGGRR526 pKa = 11.84ASFSLPSAPGAAVGIRR542 pKa = 11.84SLGALGVLVPEE553 pKa = 4.95QVVQDD558 pKa = 3.93YY559 pKa = 11.04ARR561 pKa = 11.84EE562 pKa = 4.0ALKK565 pKa = 10.22PAGKK569 pKa = 9.65VRR571 pKa = 11.84RR572 pKa = 11.84EE573 pKa = 3.74ARR575 pKa = 11.84TVEE578 pKa = 4.11LVEE581 pKa = 4.39PQLQPRR587 pKa = 11.84RR588 pKa = 11.84VPKK591 pKa = 10.56FGDD594 pKa = 3.25VGVPTSAEE602 pKa = 4.18PLRR605 pKa = 11.84GPWPAWLIGGTIAYY619 pKa = 7.56HH620 pKa = 6.93ASRR623 pKa = 11.84GDD625 pKa = 3.17RR626 pKa = 11.84DD627 pKa = 3.47AVRR630 pKa = 11.84RR631 pKa = 11.84CLDD634 pKa = 2.99TRR636 pKa = 11.84TGMWSDD642 pKa = 3.77WIRR645 pKa = 11.84RR646 pKa = 11.84NHH648 pKa = 6.65GVWTWRR654 pKa = 11.84EE655 pKa = 3.73WLRR658 pKa = 11.84GGLTPPGVTVPSWDD672 pKa = 3.59SAFLAARR679 pKa = 11.84YY680 pKa = 9.04QGWFRR685 pKa = 11.84TRR687 pKa = 11.84LRR689 pKa = 11.84AALACAGFRR698 pKa = 11.84RR699 pKa = 11.84DD700 pKa = 4.43SFINLCRR707 pKa = 11.84RR708 pKa = 11.84YY709 pKa = 9.49VLYY712 pKa = 10.99VKK714 pKa = 11.04AEE716 pKa = 4.05VTGLAWSVASS726 pKa = 4.05

Molecular weight: 81.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1884

269

889

628.0

68.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.465 ± 0.803

1.486 ± 0.731

4.989 ± 0.327

4.989 ± 0.625

3.769 ± 0.468

10.722 ± 0.677

1.433 ± 0.328

3.769 ± 0.267

2.123 ± 0.697

8.227 ± 0.531

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.911 ± 0.579

2.389 ± 0.324

6.423 ± 0.721

3.185 ± 0.114

8.439 ± 1.005

6.476 ± 0.521

4.724 ± 0.928

7.378 ± 0.631

3.45 ± 0.362

2.601 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski