Wuhan Insect virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Wuhan 4 insect cytorhabdovirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KRN7|A0A0B5KRN7_9RHAB 4b protein OS=Wuhan Insect virus 4 OX=1608109 GN=4b PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 9.97SQGFDD8 pKa = 3.26DD9 pKa = 4.89LAFGMSPHH17 pKa = 6.84HH18 pKa = 6.91NPFNDD23 pKa = 3.76DD24 pKa = 3.69ADD26 pKa = 3.92SDD28 pKa = 4.21EE29 pKa = 4.88YY30 pKa = 11.36NDD32 pKa = 4.54NVTQVPSPTVEE43 pKa = 4.62DD44 pKa = 3.84SDD46 pKa = 4.67AADD49 pKa = 3.88PSDD52 pKa = 3.75YY53 pKa = 10.43QSKK56 pKa = 10.95KK57 pKa = 10.9DD58 pKa = 3.25IDD60 pKa = 4.25QIILDD65 pKa = 4.34LNEE68 pKa = 4.05SCAEE72 pKa = 4.3LGVQTDD78 pKa = 3.47SEE80 pKa = 4.66MINAVLAMSEE90 pKa = 4.65SYY92 pKa = 11.17TMDD95 pKa = 3.26SFSLRR100 pKa = 11.84WFAFGMTYY108 pKa = 11.2ANNQRR113 pKa = 11.84IIPQLSGVLGDD124 pKa = 4.47LRR126 pKa = 11.84AEE128 pKa = 4.2IKK130 pKa = 10.58ALQQTNSSISKK141 pKa = 8.51TSSDD145 pKa = 2.89ITKK148 pKa = 10.7KK149 pKa = 9.72MVSVKK154 pKa = 10.73NDD156 pKa = 3.18VLDD159 pKa = 4.1GFEE162 pKa = 4.18KK163 pKa = 10.76LRR165 pKa = 11.84ANVLDD170 pKa = 3.95KK171 pKa = 11.29VVEE174 pKa = 4.4TSAHH178 pKa = 4.82IMARR182 pKa = 11.84DD183 pKa = 3.7EE184 pKa = 4.33NKK186 pKa = 10.29EE187 pKa = 3.84ATTQNSPSIYY197 pKa = 9.22PKK199 pKa = 9.97KK200 pKa = 10.71DD201 pKa = 3.23KK202 pKa = 10.94IEE204 pKa = 4.09LAGPTTIADD213 pKa = 3.79SQSVVMSSSPIFNDD227 pKa = 3.24PVEE230 pKa = 4.36PKK232 pKa = 9.52MVNPLLKK239 pKa = 10.21EE240 pKa = 3.81KK241 pKa = 10.72RR242 pKa = 11.84NLLIEE247 pKa = 4.4SGADD251 pKa = 3.1KK252 pKa = 10.96KK253 pKa = 10.91KK254 pKa = 10.67LRR256 pKa = 11.84EE257 pKa = 4.08LTDD260 pKa = 4.14DD261 pKa = 4.4EE262 pKa = 5.67IEE264 pKa = 4.2LLVIDD269 pKa = 4.98EE270 pKa = 4.54EE271 pKa = 5.18LEE273 pKa = 4.15LSQDD277 pKa = 3.39PTKK280 pKa = 10.75QEE282 pKa = 4.05SADD285 pKa = 3.42QLMAVLMSRR294 pKa = 11.84LLDD297 pKa = 3.53MDD299 pKa = 4.32LMM301 pKa = 5.99

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KTL5|A0A0B5KTL5_9RHAB Putative matrix protein OS=Wuhan Insect virus 4 OX=1608109 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84SGLISVNDD11 pKa = 3.82AKK13 pKa = 11.11AVVAINGSLTSFIDD27 pKa = 3.25SSKK30 pKa = 9.92VYY32 pKa = 9.99EE33 pKa = 4.23GRR35 pKa = 11.84ARR37 pKa = 11.84HH38 pKa = 5.65IARR41 pKa = 11.84KK42 pKa = 9.84RR43 pKa = 11.84EE44 pKa = 3.94VNVKK48 pKa = 9.79ILATKK53 pKa = 9.73DD54 pKa = 3.07NKK56 pKa = 10.94YY57 pKa = 11.17LMRR60 pKa = 11.84CFPLLDD66 pKa = 4.09NNDD69 pKa = 3.59VSEE72 pKa = 4.08MRR74 pKa = 11.84ADD76 pKa = 3.52KK77 pKa = 11.08KK78 pKa = 9.98NNKK81 pKa = 8.61YY82 pKa = 10.09IHH84 pKa = 6.81IGCISVSIEE93 pKa = 3.77PLIHH97 pKa = 6.72ARR99 pKa = 11.84YY100 pKa = 8.2LKK102 pKa = 10.57KK103 pKa = 10.52YY104 pKa = 7.94GKK106 pKa = 10.07EE107 pKa = 3.79MEE109 pKa = 4.86GICAIMDD116 pKa = 3.9TTFHH120 pKa = 7.58DD121 pKa = 3.68IDD123 pKa = 3.52QSIISLHH130 pKa = 6.49RR131 pKa = 11.84FDD133 pKa = 5.94LSHH136 pKa = 6.73KK137 pKa = 10.0RR138 pKa = 11.84ADD140 pKa = 5.27FICQPNHH147 pKa = 6.35SLSITDD153 pKa = 3.85DD154 pKa = 3.23NLMRR158 pKa = 11.84RR159 pKa = 11.84ISVLVIFDD167 pKa = 3.95CPKK170 pKa = 9.56VDD172 pKa = 4.24EE173 pKa = 4.74GNEE176 pKa = 3.97LFNICIGHH184 pKa = 6.43ITTCTNTLNPTSLEE198 pKa = 4.22GEE200 pKa = 4.24TSFGVIGSTPVEE212 pKa = 4.14FAAVKK217 pKa = 10.33DD218 pKa = 4.18HH219 pKa = 7.32IEE221 pKa = 3.99NALNPMGGTPCIEE234 pKa = 4.15LSEE237 pKa = 5.55DD238 pKa = 3.65GADD241 pKa = 4.61DD242 pKa = 4.95IIMKK246 pKa = 10.3KK247 pKa = 10.29KK248 pKa = 10.63SLLSKK253 pKa = 10.54VGLIKK258 pKa = 10.59APKK261 pKa = 8.83VVIRR265 pKa = 11.84KK266 pKa = 8.99NYY268 pKa = 8.83AMRR271 pKa = 11.84PRR273 pKa = 11.84EE274 pKa = 3.74HH275 pKa = 7.24DD276 pKa = 4.24RR277 pKa = 11.84IEE279 pKa = 4.22SSSDD283 pKa = 3.12LKK285 pKa = 10.93RR286 pKa = 11.84CSSLPRR292 pKa = 11.84NSLDD296 pKa = 3.46LAVARR301 pKa = 11.84VAKK304 pKa = 10.47EE305 pKa = 4.06GMFSAAFKK313 pKa = 9.33SHH315 pKa = 7.0RR316 pKa = 11.84LGGDD320 pKa = 2.66IDD322 pKa = 3.71KK323 pKa = 11.35QMILEE328 pKa = 4.21KK329 pKa = 10.49KK330 pKa = 10.26KK331 pKa = 10.64SIVRR335 pKa = 11.84NRR337 pKa = 11.84QNN339 pKa = 2.72

Molecular weight: 37.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3973

172

2105

662.2

75.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.757 ± 1.186

1.988 ± 0.417

6.343 ± 0.555

5.915 ± 0.408

3.398 ± 0.571

4.782 ± 0.367

2.391 ± 0.367

7.501 ± 0.489

7.375 ± 0.628

9.086 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.876 ± 0.333

5.311 ± 0.259

3.574 ± 0.2

2.693 ± 0.468

5.135 ± 0.649

9.665 ± 0.62

5.059 ± 0.491

5.915 ± 0.128

1.309 ± 0.284

3.927 ± 0.432
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski