Avon-Heathcote Estuary associated kieseladnavirus (AHEaBV) (Avon-Heathcote Estuary associated bacilladnavirus)
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8YT84|A0A1P8YT84_AHEBV p3 OS=Avon-Heathcote Estuary associated kieseladnavirus OX=2169964 PE=4 SV=1
MM1 pKa = 7.41 CRR3 pKa = 11.84 ASSMDD8 pKa = 3.47 SPTLRR13 pKa = 11.84 TSQVNEE19 pKa = 3.62 FLNFGDD25 pKa = 4.37 RR26 pKa = 11.84 NVHH29 pKa = 5.08 VDD31 pKa = 3.63 NIVRR35 pKa = 11.84 LLDD38 pKa = 3.65 FLVDD42 pKa = 4.07 GGSFTPQQADD52 pKa = 4.21 LYY54 pKa = 9.21 WNALYY59 pKa = 10.35 EE60 pKa = 4.51 HH61 pKa = 6.49 YY62 pKa = 10.58 HH63 pKa = 6.53 HH64 pKa = 7.02 GVLPGMEE71 pKa = 4.44 RR72 pKa = 11.84 FPLPGIDD79 pKa = 3.34 RR80 pKa = 11.84 EE81 pKa = 4.67 LYY83 pKa = 9.88 FLPALTVLFDD93 pKa = 4.0 NYY95 pKa = 10.16 IDD97 pKa = 4.89 PPTQLEE103 pKa = 4.31 SIRR106 pKa = 11.84 IEE108 pKa = 3.69 WSEE111 pKa = 4.1 EE112 pKa = 3.84 VLSDD116 pKa = 3.71 TDD118 pKa = 3.83 SLTDD122 pKa = 3.63 SEE124 pKa = 5.55 LSDD127 pKa = 3.97 LVGDD131 pKa = 4.91 IIDD134 pKa = 4.39 PDD136 pKa = 4.16 SVIDD140 pKa = 3.72 EE141 pKa = 4.61 CLLAVINGEE150 pKa = 4.1 LGDD153 pKa = 3.69 VGGDD157 pKa = 3.33 VGQDD161 pKa = 3.67 DD162 pKa = 5.76 LSDD165 pKa = 4.02 ASDD168 pKa = 3.74 GTDD171 pKa = 2.86 TLFF174 pKa = 4.91
Molecular weight: 19.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.912
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.808
Patrickios 2.664
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>sp|A0A1P8YT88|CAPSD_AHEBV Capsid protein OS=Avon-Heathcote Estuary associated kieseladnavirus OX=2169964 PE=4 SV=1
MM1 pKa = 7.63 RR2 pKa = 11.84 RR3 pKa = 11.84 TRR5 pKa = 11.84 SRR7 pKa = 11.84 SWTPGTPPRR16 pKa = 11.84 KK17 pKa = 9.4 KK18 pKa = 10.14 SEE20 pKa = 3.96 RR21 pKa = 11.84 CHH23 pKa = 5.98 VVSRR27 pKa = 11.84 DD28 pKa = 3.36 PARR31 pKa = 11.84 RR32 pKa = 11.84 LPWSRR37 pKa = 11.84 NRR39 pKa = 11.84 TDD41 pKa = 4.0 ARR43 pKa = 11.84 PSQAKK48 pKa = 9.05 PVSKK52 pKa = 10.66 KK53 pKa = 8.84 PTATALMRR61 pKa = 11.84 IPTVQAVKK69 pKa = 10.6 KK70 pKa = 9.09 EE71 pKa = 3.94 YY72 pKa = 8.83 HH73 pKa = 5.54 TALTVKK79 pKa = 10.21 QQDD82 pKa = 3.05 KK83 pKa = 10.57 RR84 pKa = 11.84 RR85 pKa = 11.84 EE86 pKa = 4.31 SKK88 pKa = 10.47 FKK90 pKa = 10.09 QALRR94 pKa = 11.84 EE95 pKa = 4.12 TKK97 pKa = 9.78 RR98 pKa = 11.84 VKK100 pKa = 10.56 DD101 pKa = 3.49 SIMRR105 pKa = 11.84 GIEE108 pKa = 3.86 YY109 pKa = 7.42 TAHH112 pKa = 5.67 VAGEE116 pKa = 4.02 VSKK119 pKa = 11.14 VVEE122 pKa = 4.13 PLALGGSAAQPEE134 pKa = 4.59 MAEE137 pKa = 4.14 FLLPAAGIADD147 pKa = 3.79 VVKK150 pKa = 10.34 RR151 pKa = 11.84 KK152 pKa = 9.49 AAIIEE157 pKa = 4.29 KK158 pKa = 8.44 TAKK161 pKa = 9.99 AAQGDD166 pKa = 3.57 RR167 pKa = 11.84 DD168 pKa = 3.98 AYY170 pKa = 10.13 KK171 pKa = 10.82 SLTDD175 pKa = 3.11 TGKK178 pKa = 8.31 YY179 pKa = 8.31 QKK181 pKa = 10.35 MMLEE185 pKa = 4.18 AKK187 pKa = 9.82 NRR189 pKa = 11.84 PKK191 pKa = 10.91 VNLIEE196 pKa = 4.05 YY197 pKa = 9.52 VPRR200 pKa = 11.84 AEE202 pKa = 4.4 DD203 pKa = 3.46 LQIEE207 pKa = 4.61 MIDD210 pKa = 3.3
Molecular weight: 23.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.206
IPC2_protein 9.531
IPC_protein 9.692
Toseland 10.613
ProMoST 10.131
Dawson 10.687
Bjellqvist 10.292
Wikipedia 10.818
Rodwell 11.169
Grimsley 10.716
Solomon 10.745
Lehninger 10.73
Nozaki 10.57
DTASelect 10.292
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.599
Patrickios 10.891
IPC_peptide 10.76
IPC2_peptide 8.77
IPC2.peptide.svr19 8.707
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1462
174
474
292.4
32.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.524 ± 0.893
1.984 ± 0.569
7.182 ± 1.275
5.267 ± 0.396
4.856 ± 0.839
5.609 ± 0.923
2.804 ± 0.421
4.993 ± 0.265
5.472 ± 1.304
8.96 ± 1.526
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.531 ± 0.615
3.967 ± 1.198
4.925 ± 0.855
3.557 ± 0.764
6.019 ± 0.881
7.25 ± 0.486
7.182 ± 0.573
5.814 ± 0.463
2.052 ± 0.454
2.052 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here