Wild vitis virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Grablovirus; Wild Vitis latent virus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223FPN7|A0A223FPN7_9GEMI V3 OS=Wild vitis virus 1 OX=2025352 PE=4 SV=1
MM1 pKa = 7.33SQTLSNAQIAQNSGIDD17 pKa = 3.77DD18 pKa = 3.83SVTIPKK24 pKa = 9.69IRR26 pKa = 11.84FDD28 pKa = 4.8PYY30 pKa = 10.92DD31 pKa = 3.35EE32 pKa = 5.2SYY34 pKa = 11.32SEE36 pKa = 4.13TSSYY40 pKa = 11.34SSCCDD45 pKa = 2.95SSRR48 pKa = 11.84GSSQSSGGGLLKK60 pKa = 10.43HH61 pKa = 6.46HH62 pKa = 6.58NSDD65 pKa = 3.29RR66 pKa = 11.84VSNSVFNEE74 pKa = 3.59LDD76 pKa = 3.67DD77 pKa = 4.45YY78 pKa = 11.94NPTLLDD84 pKa = 3.75LSLPAALWFMCEE96 pKa = 4.19RR97 pKa = 11.84YY98 pKa = 9.8LSSVVKK104 pKa = 10.59GDD106 pKa = 4.02LLVLPGISSAQCPAVRR122 pKa = 11.84RR123 pKa = 11.84LLRR126 pKa = 11.84RR127 pKa = 11.84VSRR130 pKa = 11.84RR131 pKa = 11.84NCSFHH136 pKa = 7.03SKK138 pKa = 10.05CEE140 pKa = 3.62EE141 pKa = 3.63WAYY144 pKa = 11.03GCLEE148 pKa = 4.54LKK150 pKa = 10.16ACKK153 pKa = 10.13DD154 pKa = 3.8GIEE157 pKa = 4.16SSEE160 pKa = 4.06EE161 pKa = 3.95SSEE164 pKa = 4.08KK165 pKa = 10.93SPVPAKK171 pKa = 10.23EE172 pKa = 4.11GSFEE176 pKa = 3.99AFARR180 pKa = 11.84GAACSTSS187 pKa = 2.99

Molecular weight: 20.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223FPN2|A0A223FPN2_9GEMI Replication-associated protein OS=Wild vitis virus 1 OX=2025352 PE=3 SV=1
MM1 pKa = 7.22VLRR4 pKa = 11.84ARR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84VRR10 pKa = 11.84RR11 pKa = 11.84SRR13 pKa = 11.84RR14 pKa = 11.84YY15 pKa = 7.67PRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84VLSKK23 pKa = 9.85RR24 pKa = 11.84SRR26 pKa = 11.84VARR29 pKa = 11.84RR30 pKa = 11.84ARR32 pKa = 11.84PRR34 pKa = 11.84SRR36 pKa = 11.84PCQFSFHH43 pKa = 6.54GNSFAAQPTLFFLTPIALGNGAEE66 pKa = 4.31DD67 pKa = 3.21RR68 pKa = 11.84TGPVLTVSSMYY79 pKa = 10.73LKK81 pKa = 10.8GVVIPTDD88 pKa = 3.41TVTDD92 pKa = 4.18GLHH95 pKa = 7.25DD96 pKa = 3.91VYY98 pKa = 11.03FWIILDD104 pKa = 4.36RR105 pKa = 11.84FPQGSDD111 pKa = 3.01PSYY114 pKa = 11.38GDD116 pKa = 3.23IFTGSDD122 pKa = 2.95ASGSMVEE129 pKa = 3.95TLTRR133 pKa = 11.84NKK135 pKa = 10.02QNRR138 pKa = 11.84KK139 pKa = 7.81RR140 pKa = 11.84FRR142 pKa = 11.84ILGSKK147 pKa = 9.98KK148 pKa = 10.32LVVGVNRR155 pKa = 11.84KK156 pKa = 7.89PQEE159 pKa = 4.22SLPHH163 pKa = 5.46SRR165 pKa = 11.84APFSIFQRR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84LVVSFKK181 pKa = 11.18NDD183 pKa = 3.06VTGGGRR189 pKa = 11.84NDD191 pKa = 3.22VEE193 pKa = 4.43RR194 pKa = 11.84NRR196 pKa = 11.84LYY198 pKa = 10.61LACASSTGHH207 pKa = 6.02TFRR210 pKa = 11.84LYY212 pKa = 11.05CNGIINFFNGSVFF225 pKa = 3.32

Molecular weight: 25.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1105

124

261

184.2

21.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.525 ± 0.618

2.172 ± 0.47

5.701 ± 0.952

6.244 ± 1.127

4.344 ± 0.901

5.158 ± 0.791

2.805 ± 0.699

5.882 ± 0.858

4.525 ± 0.518

7.783 ± 0.424

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.44

5.52 ± 0.575

5.792 ± 0.833

3.077 ± 0.583

8.326 ± 1.633

9.955 ± 2.008

6.154 ± 1.364

5.339 ± 0.88

0.995 ± 0.281

3.71 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski