Streptococcus phage CHPC1036
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FC15|A0A3G8FC15_9CAUD Tail fibers protein OS=Streptococcus phage CHPC1036 OX=2365012 GN=CHPC1036_0015 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.69 IEE28 pKa = 4.01 SAKK31 pKa = 10.51 QYY33 pKa = 10.21 IINAVGSDD41 pKa = 3.28 SKK43 pKa = 11.27 FYY45 pKa = 10.83 DD46 pKa = 3.78 LEE48 pKa = 4.23 NVQPLFDD55 pKa = 3.7 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.49 TVTYY78 pKa = 9.35 PINLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.4 ATYY98 pKa = 10.89 SEE100 pKa = 4.8 VVANGG105 pKa = 3.05
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.745
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.884
Rodwell 3.783
Grimsley 3.668
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.266
Thurlkill 3.808
EMBOSS 3.884
Sillero 4.062
Patrickios 0.477
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.979
Protein with the highest isoelectric point:
>tr|A0A3G8F9C3|A0A3G8F9C3_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1036 OX=2365012 GN=CHPC1036_0032 PE=4 SV=1
MM1 pKa = 7.52 TDD3 pKa = 2.86 AHH5 pKa = 6.53 KK6 pKa = 10.83 KK7 pKa = 9.38 AVKK10 pKa = 9.54 KK11 pKa = 8.98 WNKK14 pKa = 9.09 NNRR17 pKa = 11.84 EE18 pKa = 3.77 HH19 pKa = 7.64 RR20 pKa = 11.84 NYY22 pKa = 10.81 LNKK25 pKa = 10.1 RR26 pKa = 11.84 SSARR30 pKa = 11.84 GFIRR34 pKa = 11.84 NNATAEE40 pKa = 4.03 DD41 pKa = 4.03 LSEE44 pKa = 4.41 LEE46 pKa = 3.88 EE47 pKa = 5.03 LIAEE51 pKa = 4.02 RR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 10.78 KK55 pKa = 10.51 NFRR58 pKa = 3.53
Molecular weight: 6.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.225
IPC2_protein 9.589
IPC_protein 9.882
Toseland 10.818
ProMoST 10.613
Dawson 10.862
Bjellqvist 10.482
Wikipedia 11.008
Rodwell 11.286
Grimsley 10.891
Solomon 10.965
Lehninger 10.95
Nozaki 10.774
DTASelect 10.482
Thurlkill 10.774
EMBOSS 11.199
Sillero 10.789
Patrickios 11.052
IPC_peptide 10.965
IPC2_peptide 8.887
IPC2.peptide.svr19 8.79
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
10920
40
1559
227.5
25.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.584 ± 0.645
0.44 ± 0.119
6.639 ± 0.269
7.06 ± 0.545
4.203 ± 0.196
6.548 ± 0.597
1.474 ± 0.117
6.438 ± 0.235
8.819 ± 0.435
8.233 ± 0.402
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.173
6.236 ± 0.281
3.022 ± 0.203
4.46 ± 0.238
3.837 ± 0.309
6.3 ± 0.3
6.099 ± 0.408
5.595 ± 0.239
1.41 ± 0.157
4.103 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here