Streptococcus satellite phage Javan500
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUN4|A0A4D5ZUN4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan500 OX=2558726 GN=JavanS500_0021 PE=4 SV=1
MM1 pKa = 6.67 MQEE4 pKa = 4.55 LNLTPTQTLILFFVLGLLGLLLSLSKK30 pKa = 10.98 SLIDD34 pKa = 3.72 IDD36 pKa = 5.16 LPEE39 pKa = 4.68 DD40 pKa = 3.71 TQVPKK45 pKa = 10.41 PRR47 pKa = 11.84 QNANYY52 pKa = 9.31 GAYY55 pKa = 9.23 IQSQNHH61 pKa = 5.87 YY62 pKa = 9.82 YY63 pKa = 10.4 NN64 pKa = 3.73
Molecular weight: 7.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.644
IPC2_protein 5.092
IPC_protein 4.685
Toseland 4.571
ProMoST 4.851
Dawson 4.685
Bjellqvist 4.825
Wikipedia 4.609
Rodwell 4.558
Grimsley 4.495
Solomon 4.673
Lehninger 4.622
Nozaki 4.825
DTASelect 4.991
Thurlkill 4.609
EMBOSS 4.635
Sillero 4.838
Patrickios 2.066
IPC_peptide 4.673
IPC2_peptide 4.825
IPC2.peptide.svr19 4.831
Protein with the highest isoelectric point:
>tr|A0A4D5ZQ87|A0A4D5ZQ87_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan500 OX=2558726 GN=JavanS500_0012 PE=4 SV=1
MM1 pKa = 7.57 SGGGAYY7 pKa = 9.43 VCKK10 pKa = 10.2 SQKK13 pKa = 9.01 PKK15 pKa = 9.91 EE16 pKa = 4.06 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 QGLSVYY25 pKa = 7.65 EE26 pKa = 4.27 TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89 NKK47 pKa = 10.56
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.589
IPC_protein 9.692
Toseland 10.365
ProMoST 10.058
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 11.082
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.35
DTASelect 10.116
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.409
Patrickios 10.906
IPC_peptide 10.54
IPC2_peptide 8.873
IPC2.peptide.svr19 8.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
3546
40
562
154.2
17.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.753 ± 0.311
0.733 ± 0.217
5.64 ± 0.497
7.445 ± 0.543
4.005 ± 0.294
4.71 ± 0.421
2.087 ± 0.27
7.135 ± 0.347
9.334 ± 0.508
10.039 ± 0.551
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.228 ± 0.212
5.048 ± 0.235
3.299 ± 0.462
4.315 ± 0.328
5.048 ± 0.448
5.386 ± 0.29
6.486 ± 0.384
5.386 ± 0.4
0.902 ± 0.122
5.02 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here