Streptococcus satellite phage Javan500

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZUN4|A0A4D5ZUN4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan500 OX=2558726 GN=JavanS500_0021 PE=4 SV=1
MM1 pKa = 6.67MQEE4 pKa = 4.55LNLTPTQTLILFFVLGLLGLLLSLSKK30 pKa = 10.98SLIDD34 pKa = 3.72IDD36 pKa = 5.16LPEE39 pKa = 4.68DD40 pKa = 3.71TQVPKK45 pKa = 10.41PRR47 pKa = 11.84QNANYY52 pKa = 9.31GAYY55 pKa = 9.23IQSQNHH61 pKa = 5.87YY62 pKa = 9.82YY63 pKa = 10.4NN64 pKa = 3.73

Molecular weight:
7.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZQ87|A0A4D5ZQ87_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan500 OX=2558726 GN=JavanS500_0012 PE=4 SV=1
MM1 pKa = 7.57SGGGAYY7 pKa = 9.43VCKK10 pKa = 10.2SQKK13 pKa = 9.01PKK15 pKa = 9.91EE16 pKa = 4.06RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84QGLSVYY25 pKa = 7.65EE26 pKa = 4.27TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89NKK47 pKa = 10.56

Molecular weight:
5.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

3546

40

562

154.2

17.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.753 ± 0.311

0.733 ± 0.217

5.64 ± 0.497

7.445 ± 0.543

4.005 ± 0.294

4.71 ± 0.421

2.087 ± 0.27

7.135 ± 0.347

9.334 ± 0.508

10.039 ± 0.551

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.212

5.048 ± 0.235

3.299 ± 0.462

4.315 ± 0.328

5.048 ± 0.448

5.386 ± 0.29

6.486 ± 0.384

5.386 ± 0.4

0.902 ± 0.122

5.02 ± 0.444

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski