Colletotrichum shisoi

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellace

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q4BFZ3|A0A5Q4BFZ3_9PEZI Putative beta-galactosidase B (Fragment) OS=Colletotrichum shisoi OX=2078593 GN=LacB-2 PE=3 SV=1
MM1 pKa = 7.35HH2 pKa = 7.53FNRR5 pKa = 11.84VALSLAAAAAFVRR18 pKa = 11.84ADD20 pKa = 3.74LQLDD24 pKa = 3.48NDD26 pKa = 5.83DD27 pKa = 6.11IPTQCNAVCRR37 pKa = 11.84PIFSLGQACEE47 pKa = 4.21VNDD50 pKa = 4.54DD51 pKa = 4.92LVDD54 pKa = 3.79NDD56 pKa = 3.71LTEE59 pKa = 4.93DD60 pKa = 3.56RR61 pKa = 11.84LEE63 pKa = 4.32AQCVCTNNSIDD74 pKa = 3.69VAQYY78 pKa = 10.71AALCASCMDD87 pKa = 3.92QNVQDD92 pKa = 5.4RR93 pKa = 11.84DD94 pKa = 3.98DD95 pKa = 5.52LDD97 pKa = 5.29DD98 pKa = 4.65INDD101 pKa = 3.63ILRR104 pKa = 11.84TCGFASTSYY113 pKa = 11.0VATASYY119 pKa = 11.36ASTTPSVSAVRR130 pKa = 11.84PTNSAQLTTTITPGSGAGSGASPTQSSGSGSGNNNNGGGSSATPTNNGNNNQATQTPNAAGVVSPQRR197 pKa = 11.84LGLLGAAAAVGALFLL212 pKa = 4.97

Molecular weight: 21.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q4BZT5|A0A5Q4BZT5_9PEZI Benzoate 4-monooxygenase OS=Colletotrichum shisoi OX=2078593 GN=BphA PE=4 SV=1
MM1 pKa = 7.71PRR3 pKa = 11.84RR4 pKa = 11.84SSSTTPPFPTRR15 pKa = 11.84KK16 pKa = 9.72APRR19 pKa = 11.84GPSLSVRR26 pKa = 11.84HH27 pKa = 5.41STFSPASSSSPFATATAAPPLSMSSPASSASPSSSRR63 pKa = 11.84TRR65 pKa = 11.84TVTPGAPGLTAPSSTRR81 pKa = 11.84LTSPAHH87 pKa = 5.49

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11847

0

11847

5593092

9

12744

472.1

51.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.422 ± 0.025

1.18 ± 0.01

5.855 ± 0.019

6.011 ± 0.025

3.657 ± 0.013

7.267 ± 0.024

2.342 ± 0.01

4.427 ± 0.015

4.614 ± 0.026

8.648 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.162 ± 0.009

3.496 ± 0.012

6.286 ± 0.025

3.883 ± 0.02

6.312 ± 0.019

7.939 ± 0.026

5.96 ± 0.028

6.445 ± 0.021

1.47 ± 0.009

2.621 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski