Amino acid dipepetide frequency for Clostridium phage phiCD6356

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.549AlaAla: 3.549 ± 1.65
0.747AlaCys: 0.747 ± 0.233
2.708AlaAsp: 2.708 ± 0.48
4.389AlaGlu: 4.389 ± 0.757
2.055AlaPhe: 2.055 ± 0.43
2.708AlaGly: 2.708 ± 0.814
0.467AlaHis: 0.467 ± 0.184
5.136AlaIle: 5.136 ± 0.886
5.883AlaLys: 5.883 ± 1.069
5.136AlaLeu: 5.136 ± 1.204
1.121AlaMet: 1.121 ± 0.272
3.642AlaAsn: 3.642 ± 0.69
0.934AlaPro: 0.934 ± 0.293
1.494AlaGln: 1.494 ± 0.392
1.307AlaArg: 1.307 ± 0.327
2.802AlaSer: 2.802 ± 0.663
3.642AlaThr: 3.642 ± 0.648
3.362AlaVal: 3.362 ± 0.767
0.747AlaTrp: 0.747 ± 0.322
2.335AlaTyr: 2.335 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.654CysAla: 0.654 ± 0.262
0.28CysCys: 0.28 ± 0.15
0.374CysAsp: 0.374 ± 0.202
0.934CysGlu: 0.934 ± 0.33
0.84CysPhe: 0.84 ± 0.34
1.307CysGly: 1.307 ± 0.514
0.093CysHis: 0.093 ± 0.104
0.84CysIle: 0.84 ± 0.399
1.214CysLys: 1.214 ± 0.394
0.747CysLeu: 0.747 ± 0.31
0.0CysMet: 0.0 ± 0.0
0.747CysAsn: 0.747 ± 0.311
0.187CysPro: 0.187 ± 0.156
0.374CysGln: 0.374 ± 0.193
0.56CysArg: 0.56 ± 0.227
0.84CysSer: 0.84 ± 0.264
0.467CysThr: 0.467 ± 0.297
0.654CysVal: 0.654 ± 0.249
0.0CysTrp: 0.0 ± 0.0
0.374CysTyr: 0.374 ± 0.233
0.0CysXaa: 0.0 ± 0.0
Asp
3.736AspAla: 3.736 ± 0.978
0.467AspCys: 0.467 ± 0.26
3.175AspAsp: 3.175 ± 0.626
4.202AspGlu: 4.202 ± 0.696
2.335AspPhe: 2.335 ± 0.54
3.082AspGly: 3.082 ± 0.62
0.093AspHis: 0.093 ± 0.097
5.603AspIle: 5.603 ± 1.071
5.977AspLys: 5.977 ± 0.684
5.51AspLeu: 5.51 ± 0.587
1.494AspMet: 1.494 ± 0.347
4.389AspAsn: 4.389 ± 0.723
0.56AspPro: 0.56 ± 0.218
0.747AspGln: 0.747 ± 0.265
2.521AspArg: 2.521 ± 0.434
3.455AspSer: 3.455 ± 0.584
2.521AspThr: 2.521 ± 0.513
3.922AspVal: 3.922 ± 0.676
0.84AspTrp: 0.84 ± 0.239
3.175AspTyr: 3.175 ± 0.711
0.0AspXaa: 0.0 ± 0.0
Glu
4.669GluAla: 4.669 ± 1.517
1.214GluCys: 1.214 ± 0.418
4.576GluAsp: 4.576 ± 0.903
10.273GluGlu: 10.273 ± 1.323
3.829GluPhe: 3.829 ± 0.619
3.455GluGly: 3.455 ± 0.466
1.027GluHis: 1.027 ± 0.291
8.872GluIle: 8.872 ± 1.09
11.767GluLys: 11.767 ± 1.043
9.619GluLeu: 9.619 ± 1.034
2.615GluMet: 2.615 ± 0.43
8.031GluAsn: 8.031 ± 0.783
0.747GluPro: 0.747 ± 0.205
3.175GluGln: 3.175 ± 0.652
3.175GluArg: 3.175 ± 0.469
5.79GluSer: 5.79 ± 0.93
3.362GluThr: 3.362 ± 0.611
4.856GluVal: 4.856 ± 0.752
0.654GluTrp: 0.654 ± 0.26
5.51GluTyr: 5.51 ± 0.795
0.0GluXaa: 0.0 ± 0.0
Phe
2.148PheAla: 2.148 ± 0.432
0.467PheCys: 0.467 ± 0.204
3.082PheAsp: 3.082 ± 0.531
4.016PheGlu: 4.016 ± 0.56
1.401PhePhe: 1.401 ± 0.319
1.494PheGly: 1.494 ± 0.308
0.374PheHis: 0.374 ± 0.177
3.455PheIle: 3.455 ± 0.719
4.576PheLys: 4.576 ± 0.63
3.175PheLeu: 3.175 ± 0.711
1.027PheMet: 1.027 ± 0.273
3.175PheAsn: 3.175 ± 0.746
0.374PhePro: 0.374 ± 0.151
1.027PheGln: 1.027 ± 0.271
1.121PheArg: 1.121 ± 0.301
2.895PheSer: 2.895 ± 0.45
1.774PheThr: 1.774 ± 0.3
2.055PheVal: 2.055 ± 0.43
0.56PheTrp: 0.56 ± 0.218
2.708PheTyr: 2.708 ± 0.643
0.0PheXaa: 0.0 ± 0.0
Gly
3.642GlyAla: 3.642 ± 0.932
0.747GlyCys: 0.747 ± 0.303
3.175GlyAsp: 3.175 ± 0.656
4.389GlyGlu: 4.389 ± 0.582
2.148GlyPhe: 2.148 ± 0.451
2.802GlyGly: 2.802 ± 0.442
0.187GlyHis: 0.187 ± 0.118
4.483GlyIle: 4.483 ± 0.579
4.763GlyLys: 4.763 ± 0.626
4.202GlyLeu: 4.202 ± 0.582
0.84GlyMet: 0.84 ± 0.247
3.455GlyAsn: 3.455 ± 0.528
0.093GlyPro: 0.093 ± 0.076
1.214GlyGln: 1.214 ± 0.314
1.494GlyArg: 1.494 ± 0.382
2.241GlySer: 2.241 ± 0.481
2.615GlyThr: 2.615 ± 0.498
3.269GlyVal: 3.269 ± 0.543
0.934GlyTrp: 0.934 ± 0.254
1.774GlyTyr: 1.774 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
0.654HisAla: 0.654 ± 0.344
0.0HisCys: 0.0 ± 0.0
0.84HisAsp: 0.84 ± 0.313
0.654HisGlu: 0.654 ± 0.338
0.374HisPhe: 0.374 ± 0.172
0.187HisGly: 0.187 ± 0.132
0.374HisHis: 0.374 ± 0.204
0.56HisIle: 0.56 ± 0.264
1.588HisLys: 1.588 ± 0.508
0.374HisLeu: 0.374 ± 0.216
0.0HisMet: 0.0 ± 0.0
0.187HisAsn: 0.187 ± 0.144
0.28HisPro: 0.28 ± 0.162
0.187HisGln: 0.187 ± 0.123
0.374HisArg: 0.374 ± 0.161
0.747HisSer: 0.747 ± 0.267
0.84HisThr: 0.84 ± 0.369
0.56HisVal: 0.56 ± 0.189
0.0HisTrp: 0.0 ± 0.0
0.84HisTyr: 0.84 ± 0.309
0.0HisXaa: 0.0 ± 0.0
Ile
4.296IleAla: 4.296 ± 0.62
1.307IleCys: 1.307 ± 0.456
5.977IleAsp: 5.977 ± 0.781
10.086IleGlu: 10.086 ± 1.054
3.082IlePhe: 3.082 ± 0.646
3.082IleGly: 3.082 ± 0.539
0.84IleHis: 0.84 ± 0.254
4.296IleIle: 4.296 ± 0.852
12.794IleLys: 12.794 ± 1.311
7.191IleLeu: 7.191 ± 1.001
1.868IleMet: 1.868 ± 0.38
6.444IleAsn: 6.444 ± 0.787
1.961IlePro: 1.961 ± 0.505
1.401IleGln: 1.401 ± 0.317
2.802IleArg: 2.802 ± 0.445
5.323IleSer: 5.323 ± 0.593
5.883IleThr: 5.883 ± 0.801
5.136IleVal: 5.136 ± 0.521
0.374IleTrp: 0.374 ± 0.239
3.922IleTyr: 3.922 ± 0.808
0.0IleXaa: 0.0 ± 0.0
Lys
5.79LysAla: 5.79 ± 0.697
0.747LysCys: 0.747 ± 0.418
6.537LysAsp: 6.537 ± 0.861
14.195LysGlu: 14.195 ± 1.757
4.389LysPhe: 4.389 ± 0.684
6.257LysGly: 6.257 ± 0.861
1.494LysHis: 1.494 ± 0.524
11.113LysIle: 11.113 ± 0.869
12.701LysLys: 12.701 ± 1.769
9.806LysLeu: 9.806 ± 0.943
3.736LysMet: 3.736 ± 0.528
8.965LysAsn: 8.965 ± 0.85
1.214LysPro: 1.214 ± 0.315
2.988LysGln: 2.988 ± 0.851
4.389LysArg: 4.389 ± 0.57
5.323LysSer: 5.323 ± 0.689
5.697LysThr: 5.697 ± 0.805
5.977LysVal: 5.977 ± 0.704
0.84LysTrp: 0.84 ± 0.289
4.202LysTyr: 4.202 ± 0.906
0.0LysXaa: 0.0 ± 0.0
Leu
3.829LeuAla: 3.829 ± 0.89
1.027LeuCys: 1.027 ± 0.491
5.043LeuAsp: 5.043 ± 0.64
9.712LeuGlu: 9.712 ± 1.093
3.269LeuPhe: 3.269 ± 0.63
5.417LeuGly: 5.417 ± 0.73
0.56LeuHis: 0.56 ± 0.309
5.883LeuIle: 5.883 ± 0.776
10.273LeuLys: 10.273 ± 0.967
6.35LeuLeu: 6.35 ± 0.766
2.708LeuMet: 2.708 ± 0.4
6.35LeuAsn: 6.35 ± 0.737
1.681LeuPro: 1.681 ± 0.457
2.241LeuGln: 2.241 ± 0.383
2.988LeuArg: 2.988 ± 0.434
6.07LeuSer: 6.07 ± 0.878
4.389LeuThr: 4.389 ± 0.796
3.922LeuVal: 3.922 ± 0.459
0.28LeuTrp: 0.28 ± 0.163
2.055LeuTyr: 2.055 ± 0.524
0.0LeuXaa: 0.0 ± 0.0
Met
0.934MetAla: 0.934 ± 0.413
0.28MetCys: 0.28 ± 0.144
1.401MetAsp: 1.401 ± 0.305
2.148MetGlu: 2.148 ± 0.402
0.654MetPhe: 0.654 ± 0.275
1.214MetGly: 1.214 ± 0.341
0.654MetHis: 0.654 ± 0.21
2.708MetIle: 2.708 ± 0.433
3.082MetLys: 3.082 ± 0.505
1.494MetLeu: 1.494 ± 0.387
0.654MetMet: 0.654 ± 0.395
1.401MetAsn: 1.401 ± 0.381
0.654MetPro: 0.654 ± 0.234
1.027MetGln: 1.027 ± 0.285
0.374MetArg: 0.374 ± 0.181
2.055MetSer: 2.055 ± 0.398
0.934MetThr: 0.934 ± 0.341
0.56MetVal: 0.56 ± 0.18
0.467MetTrp: 0.467 ± 0.201
1.307MetTyr: 1.307 ± 0.277
0.0MetXaa: 0.0 ± 0.0
Asn
3.736AsnAla: 3.736 ± 0.608
0.654AsnCys: 0.654 ± 0.254
3.082AsnAsp: 3.082 ± 0.594
5.977AsnGlu: 5.977 ± 0.787
3.736AsnPhe: 3.736 ± 0.707
3.455AsnGly: 3.455 ± 0.68
0.934AsnHis: 0.934 ± 0.275
6.164AsnIle: 6.164 ± 0.612
8.592AsnLys: 8.592 ± 0.771
5.79AsnLeu: 5.79 ± 0.774
1.494AsnMet: 1.494 ± 0.336
5.323AsnAsn: 5.323 ± 0.657
1.681AsnPro: 1.681 ± 0.47
2.521AsnGln: 2.521 ± 0.658
2.148AsnArg: 2.148 ± 0.41
5.417AsnSer: 5.417 ± 0.858
4.296AsnThr: 4.296 ± 0.694
3.922AsnVal: 3.922 ± 0.593
0.84AsnTrp: 0.84 ± 0.235
2.802AsnTyr: 2.802 ± 0.675
0.0AsnXaa: 0.0 ± 0.0
Pro
1.681ProAla: 1.681 ± 0.332
0.0ProCys: 0.0 ± 0.0
0.374ProAsp: 0.374 ± 0.166
0.747ProGlu: 0.747 ± 0.299
1.214ProPhe: 1.214 ± 0.402
0.56ProGly: 0.56 ± 0.212
0.093ProHis: 0.093 ± 0.093
2.335ProIle: 2.335 ± 0.474
1.401ProLys: 1.401 ± 0.461
1.214ProLeu: 1.214 ± 0.331
0.28ProMet: 0.28 ± 0.146
1.121ProAsn: 1.121 ± 0.349
0.374ProPro: 0.374 ± 0.176
0.28ProGln: 0.28 ± 0.161
0.654ProArg: 0.654 ± 0.243
1.307ProSer: 1.307 ± 0.344
0.84ProThr: 0.84 ± 0.311
1.027ProVal: 1.027 ± 0.275
0.093ProTrp: 0.093 ± 0.086
0.187ProTyr: 0.187 ± 0.124
0.0ProXaa: 0.0 ± 0.0
Gln
1.588GlnAla: 1.588 ± 0.682
0.187GlnCys: 0.187 ± 0.141
1.588GlnAsp: 1.588 ± 0.325
3.175GlnGlu: 3.175 ± 0.83
0.654GlnPhe: 0.654 ± 0.255
1.121GlnGly: 1.121 ± 0.374
0.093GlnHis: 0.093 ± 0.095
2.428GlnIle: 2.428 ± 0.493
2.802GlnLys: 2.802 ± 0.559
2.708GlnLeu: 2.708 ± 0.523
0.747GlnMet: 0.747 ± 0.218
1.588GlnAsn: 1.588 ± 0.316
0.187GlnPro: 0.187 ± 0.121
1.401GlnGln: 1.401 ± 0.545
0.56GlnArg: 0.56 ± 0.298
1.494GlnSer: 1.494 ± 0.35
1.494GlnThr: 1.494 ± 0.277
1.401GlnVal: 1.401 ± 0.327
0.093GlnTrp: 0.093 ± 0.098
0.187GlnTyr: 0.187 ± 0.131
0.0GlnXaa: 0.0 ± 0.0
Arg
1.401ArgAla: 1.401 ± 0.381
0.187ArgCys: 0.187 ± 0.139
2.055ArgAsp: 2.055 ± 0.626
3.269ArgGlu: 3.269 ± 0.519
1.027ArgPhe: 1.027 ± 0.284
1.961ArgGly: 1.961 ± 0.478
0.56ArgHis: 0.56 ± 0.252
3.082ArgIle: 3.082 ± 0.51
3.549ArgLys: 3.549 ± 0.592
2.895ArgLeu: 2.895 ± 0.507
0.934ArgMet: 0.934 ± 0.308
2.241ArgAsn: 2.241 ± 0.38
0.467ArgPro: 0.467 ± 0.231
0.654ArgGln: 0.654 ± 0.251
0.747ArgArg: 0.747 ± 0.259
1.868ArgSer: 1.868 ± 0.364
2.428ArgThr: 2.428 ± 0.45
1.961ArgVal: 1.961 ± 0.358
0.56ArgTrp: 0.56 ± 0.184
1.214ArgTyr: 1.214 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
3.362SerAla: 3.362 ± 0.632
0.654SerCys: 0.654 ± 0.399
3.642SerAsp: 3.642 ± 0.541
4.95SerGlu: 4.95 ± 0.599
2.428SerPhe: 2.428 ± 0.489
2.708SerGly: 2.708 ± 0.642
0.187SerHis: 0.187 ± 0.13
7.471SerIle: 7.471 ± 1.1
6.911SerLys: 6.911 ± 0.849
4.202SerLeu: 4.202 ± 0.645
1.494SerMet: 1.494 ± 0.513
4.856SerAsn: 4.856 ± 0.815
1.027SerPro: 1.027 ± 0.297
1.401SerGln: 1.401 ± 0.363
1.868SerArg: 1.868 ± 0.431
3.922SerSer: 3.922 ± 0.699
4.109SerThr: 4.109 ± 0.921
2.615SerVal: 2.615 ± 0.354
0.654SerTrp: 0.654 ± 0.22
2.615SerTyr: 2.615 ± 0.56
0.0SerXaa: 0.0 ± 0.0
Thr
3.922ThrAla: 3.922 ± 1.137
0.654ThrCys: 0.654 ± 0.233
2.708ThrAsp: 2.708 ± 0.435
4.202ThrGlu: 4.202 ± 0.551
2.615ThrPhe: 2.615 ± 0.491
2.708ThrGly: 2.708 ± 0.478
0.56ThrHis: 0.56 ± 0.286
5.23ThrIle: 5.23 ± 0.649
6.257ThrLys: 6.257 ± 0.705
4.296ThrLeu: 4.296 ± 0.534
0.934ThrMet: 0.934 ± 0.32
3.642ThrAsn: 3.642 ± 0.579
1.494ThrPro: 1.494 ± 0.381
1.494ThrGln: 1.494 ± 0.388
1.681ThrArg: 1.681 ± 0.379
3.175ThrSer: 3.175 ± 0.528
4.202ThrThr: 4.202 ± 0.879
3.082ThrVal: 3.082 ± 0.535
0.747ThrTrp: 0.747 ± 0.312
1.401ThrTyr: 1.401 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
2.615ValAla: 2.615 ± 0.596
1.027ValCys: 1.027 ± 0.349
3.829ValAsp: 3.829 ± 0.486
5.23ValGlu: 5.23 ± 0.915
2.055ValPhe: 2.055 ± 0.495
2.988ValGly: 2.988 ± 0.604
0.747ValHis: 0.747 ± 0.265
3.175ValIle: 3.175 ± 0.529
6.164ValLys: 6.164 ± 0.778
5.51ValLeu: 5.51 ± 0.771
0.934ValMet: 0.934 ± 0.297
2.895ValAsn: 2.895 ± 0.533
1.307ValPro: 1.307 ± 0.398
1.121ValGln: 1.121 ± 0.318
1.681ValArg: 1.681 ± 0.367
3.269ValSer: 3.269 ± 0.681
3.175ValThr: 3.175 ± 0.561
3.922ValVal: 3.922 ± 0.536
0.28ValTrp: 0.28 ± 0.159
2.428ValTyr: 2.428 ± 0.591
0.0ValXaa: 0.0 ± 0.0
Trp
0.467TrpAla: 0.467 ± 0.156
0.093TrpCys: 0.093 ± 0.105
0.747TrpAsp: 0.747 ± 0.279
1.401TrpGlu: 1.401 ± 0.789
0.467TrpPhe: 0.467 ± 0.158
0.467TrpGly: 0.467 ± 0.185
0.0TrpHis: 0.0 ± 0.0
0.467TrpIle: 0.467 ± 0.213
0.467TrpLys: 0.467 ± 0.204
0.747TrpLeu: 0.747 ± 0.297
0.093TrpMet: 0.093 ± 0.125
0.747TrpAsn: 0.747 ± 0.283
0.0TrpPro: 0.0 ± 0.0
0.187TrpGln: 0.187 ± 0.114
0.374TrpArg: 0.374 ± 0.184
0.747TrpSer: 0.747 ± 0.234
0.56TrpThr: 0.56 ± 0.198
0.56TrpVal: 0.56 ± 0.186
0.187TrpTrp: 0.187 ± 0.144
0.56TrpTyr: 0.56 ± 0.228
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.494TyrAla: 1.494 ± 0.277
0.747TyrCys: 0.747 ± 0.302
2.895TyrAsp: 2.895 ± 0.456
3.269TyrGlu: 3.269 ± 0.592
2.335TyrPhe: 2.335 ± 0.642
1.494TyrGly: 1.494 ± 0.422
0.28TyrHis: 0.28 ± 0.166
4.763TyrIle: 4.763 ± 0.983
5.603TyrLys: 5.603 ± 0.823
3.082TyrLeu: 3.082 ± 0.59
0.934TyrMet: 0.934 ± 0.286
3.362TyrAsn: 3.362 ± 0.645
0.56TyrPro: 0.56 ± 0.218
0.56TyrGln: 0.56 ± 0.266
2.241TyrArg: 2.241 ± 0.621
2.335TyrSer: 2.335 ± 0.496
1.774TyrThr: 1.774 ± 0.485
1.588TyrVal: 1.588 ± 0.453
0.28TyrTrp: 0.28 ± 0.161
1.588TyrTyr: 1.588 ± 0.355
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (10709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski