Acinetobacter sp. CAG:196

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; environmental samples

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5SHP1|R5SHP1_9GAMM Uncharacterized protein OS=Acinetobacter sp. CAG:196 OX=1262690 GN=BN527_00523 PE=4 SV=1
MM1 pKa = 7.39GNDD4 pKa = 3.27IEE6 pKa = 4.36SLQNILKK13 pKa = 9.99NDD15 pKa = 3.59PSNFQARR22 pKa = 11.84RR23 pKa = 11.84EE24 pKa = 3.94LSILLVNNGFNEE36 pKa = 4.11EE37 pKa = 4.16AEE39 pKa = 4.5GNLKK43 pKa = 10.46YY44 pKa = 10.56LLKK47 pKa = 10.72YY48 pKa = 10.24FPQDD52 pKa = 2.92AEE54 pKa = 3.81IYY56 pKa = 8.27YY57 pKa = 10.59NLGIVYY63 pKa = 9.19EE64 pKa = 4.1KK65 pKa = 10.73LKK67 pKa = 10.96KK68 pKa = 8.91FTEE71 pKa = 4.34AKK73 pKa = 9.56EE74 pKa = 4.42CYY76 pKa = 9.02QKK78 pKa = 11.04AVEE81 pKa = 4.71ISPQEE86 pKa = 3.73DD87 pKa = 3.92FYY89 pKa = 12.01YY90 pKa = 11.12NLGDD94 pKa = 3.55VLVDD98 pKa = 3.9LKK100 pKa = 10.99EE101 pKa = 3.75WDD103 pKa = 3.58EE104 pKa = 4.76AISAFRR110 pKa = 11.84KK111 pKa = 10.0VLEE114 pKa = 4.41TDD116 pKa = 3.71PEE118 pKa = 4.68DD119 pKa = 3.91GNCYY123 pKa = 10.14FNLGVCYY130 pKa = 10.28FNKK133 pKa = 10.23DD134 pKa = 3.68EE135 pKa = 5.64KK136 pKa = 11.35NLALDD141 pKa = 4.04NFQKK145 pKa = 10.87AAAINPKK152 pKa = 10.41DD153 pKa = 3.58VFAYY157 pKa = 9.46FYY159 pKa = 11.05LGFIYY164 pKa = 10.74QNDD167 pKa = 4.02GLTNFAIDD175 pKa = 3.48SYY177 pKa = 11.46KK178 pKa = 10.47KK179 pKa = 10.4VLEE182 pKa = 4.04ISPDD186 pKa = 3.52YY187 pKa = 10.87SWAYY191 pKa = 10.5FNLGSIAYY199 pKa = 8.93KK200 pKa = 10.26SGNIEE205 pKa = 3.7EE206 pKa = 4.23AKK208 pKa = 10.44EE209 pKa = 3.89YY210 pKa = 10.78LLKK213 pKa = 9.98TLEE216 pKa = 4.26YY217 pKa = 10.16NSSDD221 pKa = 2.93IEE223 pKa = 4.22AYY225 pKa = 9.75KK226 pKa = 10.89LLTKK230 pKa = 10.13ICLKK234 pKa = 10.04TGEE237 pKa = 4.4TEE239 pKa = 4.28EE240 pKa = 4.16ILEE243 pKa = 4.15ILNTRR248 pKa = 11.84LDD250 pKa = 3.69KK251 pKa = 11.13EE252 pKa = 4.72DD253 pKa = 4.48NGDD256 pKa = 4.01LYY258 pKa = 11.63YY259 pKa = 11.12CLARR263 pKa = 11.84VYY265 pKa = 10.55KK266 pKa = 10.55YY267 pKa = 10.49IGDD270 pKa = 3.83SEE272 pKa = 4.68QYY274 pKa = 10.48CKK276 pKa = 10.71NLEE279 pKa = 4.03QALDD283 pKa = 3.62NPYY286 pKa = 9.25TLTFSKK292 pKa = 10.52KK293 pKa = 9.01IVKK296 pKa = 9.85QEE298 pKa = 3.69LEE300 pKa = 4.42YY301 pKa = 10.57IKK303 pKa = 11.04EE304 pKa = 4.0MLGRR308 pKa = 11.84NAVIEE313 pKa = 4.14PEE315 pKa = 4.0AGIEE319 pKa = 4.24EE320 pKa = 4.47YY321 pKa = 10.7SEE323 pKa = 4.19EE324 pKa = 4.13EE325 pKa = 4.15PEE327 pKa = 3.85EE328 pKa = 4.29DD329 pKa = 3.98EE330 pKa = 4.9NYY332 pKa = 10.5EE333 pKa = 4.99DD334 pKa = 3.77EE335 pKa = 5.18PEE337 pKa = 4.3YY338 pKa = 11.13DD339 pKa = 4.52DD340 pKa = 5.4EE341 pKa = 6.76ADD343 pKa = 3.67EE344 pKa = 4.45YY345 pKa = 11.44EE346 pKa = 4.18EE347 pKa = 6.29DD348 pKa = 4.53EE349 pKa = 6.15DD350 pKa = 5.06YY351 pKa = 11.66DD352 pKa = 5.09DD353 pKa = 6.49DD354 pKa = 5.76GEE356 pKa = 4.17EE357 pKa = 4.37DD358 pKa = 3.53SS359 pKa = 5.06

Molecular weight: 41.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5SSV7|R5SSV7_9GAMM Pyruvate formate-lyase-activating enzyme OS=Acinetobacter sp. CAG:196 OX=1262690 GN=BN527_02002 PE=3 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84LVGVDD8 pKa = 3.47LPRR11 pKa = 11.84NKK13 pKa = 10.1RR14 pKa = 11.84LEE16 pKa = 3.77IALTYY21 pKa = 10.09IYY23 pKa = 10.47GIGPARR29 pKa = 11.84SKK31 pKa = 10.88KK32 pKa = 9.0ILEE35 pKa = 4.24VTGISPDD42 pKa = 3.68LRR44 pKa = 11.84TDD46 pKa = 3.84DD47 pKa = 5.1LTDD50 pKa = 4.72DD51 pKa = 5.67DD52 pKa = 5.33IKK54 pKa = 10.99QLRR57 pKa = 11.84NALSEE62 pKa = 4.15YY63 pKa = 10.54NIEE66 pKa = 3.93GDD68 pKa = 3.53LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.58VTMNIKK78 pKa = 10.21RR79 pKa = 11.84LQEE82 pKa = 3.81INSYY86 pKa = 10.63RR87 pKa = 11.84GMRR90 pKa = 11.84HH91 pKa = 6.02KK92 pKa = 10.66RR93 pKa = 11.84GLPCRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.84TNARR107 pKa = 11.84TRR109 pKa = 11.84RR110 pKa = 11.84GKK112 pKa = 8.91KK113 pKa = 7.98TGPIAGKK120 pKa = 10.3KK121 pKa = 8.86KK122 pKa = 10.5

Molecular weight: 13.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2085

0

2085

636768

31

2806

305.4

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.013 ± 0.049

1.244 ± 0.026

5.631 ± 0.041

7.053 ± 0.072

4.438 ± 0.048

6.107 ± 0.069

1.413 ± 0.019

8.366 ± 0.05

8.539 ± 0.068

9.047 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.029

6.215 ± 0.079

3.22 ± 0.035

3.132 ± 0.035

3.536 ± 0.043

6.302 ± 0.063

5.406 ± 0.062

6.128 ± 0.041

0.671 ± 0.014

4.022 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski