Hydrogenovibrio marinus
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2330 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A067A105|A0A067A105_HYDMR Pribosyltran domain-containing protein OS=Hydrogenovibrio marinus OX=28885 GN=EI16_08415 PE=4 SV=1
MM1 pKa = 7.04 ITNITDD7 pKa = 3.49 TNGDD11 pKa = 3.6 YY12 pKa = 11.43 SNVILHH18 pKa = 5.88 GTGEE22 pKa = 4.17 PGATVTLLSRR32 pKa = 11.84 EE33 pKa = 3.99 GSTTGGNDD41 pKa = 3.12 TGSWTYY47 pKa = 9.79 TAVEE51 pKa = 4.19 TSTPIVVDD59 pKa = 4.36 ANGNWTVDD67 pKa = 2.99 ISNLPNTPIDD77 pKa = 3.84 DD78 pKa = 4.09 NEE80 pKa = 4.23 FFKK83 pKa = 10.62 ATQIDD88 pKa = 3.5 ASGNVSADD96 pKa = 3.42 SDD98 pKa = 4.35 SVHH101 pKa = 5.94 YY102 pKa = 9.07 WHH104 pKa = 7.55 GDD106 pKa = 2.85 WSSVATEE113 pKa = 4.26 ANDD116 pKa = 3.82 DD117 pKa = 3.92 YY118 pKa = 12.08 VMMGSGKK125 pKa = 9.79 DD126 pKa = 3.33 QIIISSNDD134 pKa = 3.44 TNDD137 pKa = 3.11 HH138 pKa = 5.69 FVVDD142 pKa = 4.32 GGDD145 pKa = 3.89 NIDD148 pKa = 3.51 TAVFSGKK155 pKa = 10.18 FSDD158 pKa = 4.35 YY159 pKa = 11.43 ALSTSSTGSLIVTEE173 pKa = 4.9 GVSTDD178 pKa = 3.3 SNGNGVGDD186 pKa = 3.62 VDD188 pKa = 3.69 EE189 pKa = 5.02 LRR191 pKa = 11.84 NIEE194 pKa = 4.27 KK195 pKa = 10.83 VKK197 pKa = 9.85 FTDD200 pKa = 3.36 GTYY203 pKa = 10.64 DD204 pKa = 3.18 VPTGVFKK211 pKa = 11.43 LNGISVDD218 pKa = 3.52 IEE220 pKa = 4.43 HH221 pKa = 7.33 DD222 pKa = 3.55 TGTSSTDD229 pKa = 3.23 RR230 pKa = 11.84 ITNDD234 pKa = 2.56 STLKK238 pKa = 9.37 VTGLVSGATIEE249 pKa = 4.14 YY250 pKa = 10.35 SSDD253 pKa = 3.25 GGQTWTTQYY262 pKa = 11.28 NPQEE266 pKa = 4.2 GVNNISVRR274 pKa = 11.84 QVDD277 pKa = 4.24 AEE279 pKa = 4.25 GHH281 pKa = 5.86 TSDD284 pKa = 4.25 SSNVSFTLDD293 pKa = 3.24 TQAGANIDD301 pKa = 3.65 VDD303 pKa = 3.6 ILTGDD308 pKa = 4.2 GYY310 pKa = 10.06 LTAAEE315 pKa = 4.58 AFPGVKK321 pKa = 10.07 VPVTGYY327 pKa = 10.11 VQGDD331 pKa = 4.0 AQPGDD336 pKa = 4.12 TISIILDD343 pKa = 3.38 GNVIGTGTVSSDD355 pKa = 3.46 VNSSGAYY362 pKa = 7.39 TFSVDD367 pKa = 4.35 VLGSDD372 pKa = 3.78 LADD375 pKa = 3.78 GSGMIPEE382 pKa = 5.04 LKK384 pKa = 10.02 ATVTGSDD391 pKa = 3.29 VAGNTFSAEE400 pKa = 3.98 STEE403 pKa = 4.79 VYY405 pKa = 9.95 MKK407 pKa = 10.94 DD408 pKa = 4.12 FIATVSINVTDD419 pKa = 4.52 GNTSDD424 pKa = 3.6 SDD426 pKa = 3.95 NVISASEE433 pKa = 3.87 ASSTVVSGNVEE444 pKa = 3.6 VGGQVSSITISDD456 pKa = 3.69 GTNTLTIPANQITVYY471 pKa = 10.9 SNGYY475 pKa = 7.37 YY476 pKa = 9.38 TVNTDD481 pKa = 3.29 VSSLNDD487 pKa = 3.5 GVLTVSVEE495 pKa = 4.21 ASDD498 pKa = 3.83 KK499 pKa = 11.02 LGNVGTNSTTIEE511 pKa = 4.71 KK512 pKa = 8.78 DD513 pKa = 3.18 TQAQAGTVTVDD524 pKa = 4.87 SITDD528 pKa = 3.64 DD529 pKa = 4.31 NIVNTMEE536 pKa = 4.25 SGQTIAVTGTATGGDD551 pKa = 3.54 ISSGDD556 pKa = 3.94 TLTMTINGKK565 pKa = 10.19 DD566 pKa = 3.36 YY567 pKa = 10.35 STTVNSDD574 pKa = 3.51 GNWSVDD580 pKa = 3.45 VAGSDD585 pKa = 3.97 LAADD589 pKa = 3.81 ADD591 pKa = 3.97 KK592 pKa = 11.56 GFTASVASSDD602 pKa = 3.53 AAGNTVTSSAAHH614 pKa = 6.71 SYY616 pKa = 9.41 TVDD619 pKa = 3.58 SQPEE623 pKa = 4.24 GHH625 pKa = 7.44 DD626 pKa = 3.34 EE627 pKa = 4.11 TQTMDD632 pKa = 3.64 FEE634 pKa = 4.84 SATVSQKK641 pKa = 6.25 TTNVVITLDD650 pKa = 3.3 ISGSMDD656 pKa = 3.29 SDD658 pKa = 3.7 SRR660 pKa = 11.84 LALAKK665 pKa = 10.37 SALEE669 pKa = 3.96 KK670 pKa = 10.34 MINAYY675 pKa = 10.28 DD676 pKa = 3.54 NQGNVNVKK684 pKa = 9.92 LVAFNGGATLEE695 pKa = 4.11 QHH697 pKa = 7.15 DD698 pKa = 4.14 GSVWLKK704 pKa = 10.29 ADD706 pKa = 3.62 EE707 pKa = 5.27 AISIIKK713 pKa = 9.71 DD714 pKa = 3.49 LQAGGSTNYY723 pKa = 10.09 EE724 pKa = 3.9 DD725 pKa = 5.71 AVLKK729 pKa = 10.06 TYY731 pKa = 11.2 SNYY734 pKa = 10.11 SEE736 pKa = 4.02 PTADD740 pKa = 2.96 KK741 pKa = 9.75 TVAYY745 pKa = 8.72 FISDD749 pKa = 4.15 GEE751 pKa = 4.39 PTTEE755 pKa = 3.8 NYY757 pKa = 10.22 GGNGSGTAGGFLDD770 pKa = 4.36 NDD772 pKa = 4.32 YY773 pKa = 11.58 EE774 pKa = 4.48 HH775 pKa = 6.99 GWDD778 pKa = 3.62 NFVSQYY784 pKa = 11.01 VDD786 pKa = 3.54 DD787 pKa = 4.41 LHH789 pKa = 8.49 VVALGDD795 pKa = 3.58 DD796 pKa = 4.0 ALNSKK801 pKa = 10.15 YY802 pKa = 10.86 LDD804 pKa = 3.59 VLASAGNNVSTVTEE818 pKa = 4.09 VSDD821 pKa = 4.5 ASQLSDD827 pKa = 5.12 AIVPTNTEE835 pKa = 3.84 LSVTGTVSDD844 pKa = 4.09 NVTGGDD850 pKa = 3.72 GAITIQSITVDD861 pKa = 3.34 GTAYY865 pKa = 9.67 TSSTFPSEE873 pKa = 3.72 GVAINGKK880 pKa = 8.33 GTLAFDD886 pKa = 4.76 FSTGNYY892 pKa = 7.53 TYY894 pKa = 10.93 SAGSNEE900 pKa = 3.81 FTKK903 pKa = 10.74 DD904 pKa = 3.19 VTQMFTVTAADD915 pKa = 3.83 ADD917 pKa = 4.15 GDD919 pKa = 4.1 TADD922 pKa = 3.74 VKK924 pKa = 11.29 VSINVNVDD932 pKa = 3.24 DD933 pKa = 4.25 TASNATLSMSIGDD946 pKa = 4.06 AEE948 pKa = 4.49 VTTTHH953 pKa = 6.74 NYY955 pKa = 9.96 TYY957 pKa = 11.02 DD958 pKa = 3.42 SDD960 pKa = 3.97 DD961 pKa = 3.63 ARR963 pKa = 11.84 NANHH967 pKa = 7.26 DD968 pKa = 4.21 NYY970 pKa = 10.99 HH971 pKa = 6.11 SLHH974 pKa = 7.03 DD975 pKa = 4.43 ALEE978 pKa = 4.48 GQNGCNHH985 pKa = 6.29 SNGTLVYY992 pKa = 10.42 KK993 pKa = 10.57 NVNEE997 pKa = 4.33 TSWNLSDD1004 pKa = 5.79 SSDD1007 pKa = 3.36 KK1008 pKa = 11.24 LFAITGNSHH1017 pKa = 6.06 NANINLSGGNNTLIFEE1033 pKa = 5.01 KK1034 pKa = 10.89 DD1035 pKa = 3.23 PGSNIAVNFGSGKK1048 pKa = 10.25 DD1049 pKa = 3.63 VLILPGSEE1057 pKa = 3.58 SDD1059 pKa = 3.86 YY1060 pKa = 11.18 NLSAFHH1066 pKa = 6.79 NNGGVLSGQITGHH1079 pKa = 6.94 DD1080 pKa = 3.63 NMNLTVNNLDD1090 pKa = 3.84 CLVFSDD1096 pKa = 4.64 QVMGDD1101 pKa = 3.06 SSLYY1105 pKa = 10.38 KK1106 pKa = 10.82 GEE1108 pKa = 4.17 TSNHH1112 pKa = 3.83 YY1113 pKa = 9.61 TYY1115 pKa = 11.16 DD1116 pKa = 3.73 LSASASLTDD1125 pKa = 3.53 TDD1127 pKa = 4.29 GSEE1130 pKa = 4.18 SLSDD1134 pKa = 3.28 ITISGLPDD1142 pKa = 4.5 DD1143 pKa = 5.35 GSVTVTGTDD1152 pKa = 3.04 VSKK1155 pKa = 11.49 NDD1157 pKa = 3.51 DD1158 pKa = 2.97 GTYY1161 pKa = 10.3 NVKK1164 pKa = 10.46 LQDD1167 pKa = 4.01 DD1168 pKa = 4.44 GKK1170 pKa = 10.71 IADD1173 pKa = 4.23 DD1174 pKa = 3.97 VKK1176 pKa = 10.72 ISSSKK1181 pKa = 10.54 EE1182 pKa = 3.82 LTSSEE1187 pKa = 4.36 LNDD1190 pKa = 3.01 VHH1192 pKa = 8.98 ASVTSTEE1199 pKa = 4.41 SNGNEE1204 pKa = 3.96 TATTEE1209 pKa = 4.21 VNEE1212 pKa = 4.4 SGDD1215 pKa = 3.49 NFLYY1219 pKa = 10.86 ADD1221 pKa = 3.84 TGDD1224 pKa = 4.08 DD1225 pKa = 4.12 LFVGTNQSDD1234 pKa = 4.12 TIKK1237 pKa = 10.66 VDD1239 pKa = 3.33 GDD1241 pKa = 3.62 GSTTVQNFDD1250 pKa = 3.37 VQNDD1254 pKa = 3.64 VLDD1257 pKa = 4.52 LSDD1260 pKa = 5.48 VIADD1264 pKa = 4.01 DD1265 pKa = 4.21 QVVTQDD1271 pKa = 3.62 TLSQYY1276 pKa = 9.97 LTVTQTDD1283 pKa = 3.14 KK1284 pKa = 10.71 GTEE1287 pKa = 3.96 VTVDD1291 pKa = 3.47 SNGKK1295 pKa = 9.26 DD1296 pKa = 3.38 VAGGEE1301 pKa = 4.37 VANIMLEE1308 pKa = 3.91 GLNDD1312 pKa = 3.98 SNNLQIQIDD1321 pKa = 4.07 DD1322 pKa = 3.99 NKK1324 pKa = 10.44 VDD1326 pKa = 3.52 YY1327 pKa = 11.12 HH1328 pKa = 6.78 DD1329 pKa = 3.94
Molecular weight: 138.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.855
IPC2_protein 3.617
IPC_protein 3.694
Toseland 3.439
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.101
Thurlkill 3.503
EMBOSS 3.656
Sillero 3.821
Patrickios 2.524
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A066ZQP2|A0A066ZQP2_HYDMR Rhodanese domain-containing protein OS=Hydrogenovibrio marinus OX=28885 GN=EI16_05825 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.98 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2330
0
2330
736326
41
2103
316.0
35.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.278 ± 0.051
0.834 ± 0.018
5.602 ± 0.043
6.541 ± 0.052
4.254 ± 0.037
6.563 ± 0.047
2.302 ± 0.024
6.383 ± 0.04
6.133 ± 0.045
10.199 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.853 ± 0.026
4.237 ± 0.032
3.974 ± 0.029
4.681 ± 0.045
4.237 ± 0.037
6.416 ± 0.041
5.246 ± 0.039
6.994 ± 0.048
1.229 ± 0.023
3.044 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here