candidate division MSBL1 archaeon SCGC-AAA259M10

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UZA2|A0A133UZA2_9EURY Uncharacterized protein (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA259M10 OX=1698270 GN=AKJ40_02970 PE=4 SV=1
MM1 pKa = 7.12NRR3 pKa = 11.84SGTNKK8 pKa = 9.07TLLFLIITVTCFMVAASLPVVMGDD32 pKa = 3.99TIDD35 pKa = 4.59GEE37 pKa = 4.42ASVSNTSCEE46 pKa = 4.56FIDD49 pKa = 4.03KK50 pKa = 9.78TVDD53 pKa = 3.12TNTVDD58 pKa = 3.28PEE60 pKa = 4.5SYY62 pKa = 10.35FWIKK66 pKa = 8.37TQVRR70 pKa = 11.84DD71 pKa = 3.58SDD73 pKa = 3.95TLSDD77 pKa = 3.63IEE79 pKa = 4.23NVVVKK84 pKa = 10.81IFDD87 pKa = 3.43NQTAVGAQDD96 pKa = 3.65TKK98 pKa = 10.53RR99 pKa = 11.84SHH101 pKa = 4.15YY102 pKa = 8.61TFWFDD107 pKa = 5.43ANDD110 pKa = 3.62NSWNSNLNNTLGNPFIDD127 pKa = 4.08AAASEE132 pKa = 4.68YY133 pKa = 10.66PNDD136 pKa = 3.7ATVTEE141 pKa = 4.58DD142 pKa = 3.14NYY144 pKa = 11.42NFKK147 pKa = 10.54ILLNGTANPNQDD159 pKa = 2.4WNVYY163 pKa = 10.07VEE165 pKa = 4.53VNDD168 pKa = 3.92EE169 pKa = 4.22ASSEE173 pKa = 4.16NNGTHH178 pKa = 5.84TNALAVNTYY187 pKa = 10.3VNYY190 pKa = 10.07DD191 pKa = 3.38VKK193 pKa = 11.35ADD195 pKa = 4.44LIEE198 pKa = 4.15WTNLQPDD205 pKa = 4.22TNDD208 pKa = 3.51NPSDD212 pKa = 3.98SNPVIIEE219 pKa = 4.26NIEE222 pKa = 4.1TNVNYY227 pKa = 10.32DD228 pKa = 3.34VQTKK232 pKa = 10.75LSGDD236 pKa = 3.49WLNTNGDD243 pKa = 4.31TITVTEE249 pKa = 4.26TSFDD253 pKa = 3.73STDD256 pKa = 2.79SSGYY260 pKa = 9.18HH261 pKa = 5.47GQVSSSIFKK270 pKa = 10.43DD271 pKa = 3.44VYY273 pKa = 9.46TYY275 pKa = 10.99EE276 pKa = 4.44SYY278 pKa = 11.57GEE280 pKa = 4.45DD281 pKa = 3.06LTEE284 pKa = 4.64GINYY288 pKa = 9.45FLDD291 pKa = 3.96IPDD294 pKa = 4.12GTSSGTFTNTFEE306 pKa = 4.49IEE308 pKa = 4.56VVAHH312 pKa = 6.77

Molecular weight: 34.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133UYJ2|A0A133UYJ2_9EURY 4Fe-4S ferredoxin (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA259M10 OX=1698270 GN=AKJ40_03640 PE=4 SV=1
MM1 pKa = 7.39RR2 pKa = 11.84RR3 pKa = 11.84EE4 pKa = 4.24GSWAWADD11 pKa = 3.45MLLDD15 pKa = 5.29FIEE18 pKa = 5.44NPQKK22 pKa = 10.3WLRR25 pKa = 11.84EE26 pKa = 3.79CHH28 pKa = 5.59TRR30 pKa = 11.84SNVEE34 pKa = 4.05SGFSTFKK41 pKa = 10.72RR42 pKa = 11.84HH43 pKa = 5.87FLSPLRR49 pKa = 11.84KK50 pKa = 9.5CIQRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84KK57 pKa = 7.84TEE59 pKa = 3.53AFARR63 pKa = 11.84ACDD66 pKa = 3.98YY67 pKa = 10.69NLKK70 pKa = 9.63RR71 pKa = 11.84ASYY74 pKa = 10.09VRR76 pKa = 11.84RR77 pKa = 11.84QEE79 pKa = 3.98GLTAA83 pKa = 4.79

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

893

0

893

200716

33

1234

224.8

25.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.858 ± 0.082

1.003 ± 0.034

5.787 ± 0.064

10.172 ± 0.123

4.036 ± 0.072

7.458 ± 0.085

1.693 ± 0.039

7.013 ± 0.08

7.561 ± 0.098

9.254 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.036

3.747 ± 0.052

4.05 ± 0.053

2.206 ± 0.042

5.755 ± 0.081

6.382 ± 0.065

4.823 ± 0.068

6.878 ± 0.061

1.098 ± 0.034

3.029 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski