Ileibacterium valens
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2365 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U7NFL9|A0A1U7NFL9_9FIRM Uncharacterized protein OS=Ileibacterium valens OX=1862668 GN=BO222_07005 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 9.15 FTKK5 pKa = 10.5 LFTAFSLACLLAACSSNTPASDD27 pKa = 3.34 SNTNHH32 pKa = 6.59 AQNQTSVNDD41 pKa = 3.8 EE42 pKa = 4.16 KK43 pKa = 10.75 TSDD46 pKa = 3.87 DD47 pKa = 3.78 TNTNNNSSNSSNSSNTNSNQNSGNAYY73 pKa = 9.05 AAGSEE78 pKa = 4.32 FEE80 pKa = 4.31 NAFANAGYY88 pKa = 10.34 DD89 pKa = 3.47 IIHH92 pKa = 6.74 TEE94 pKa = 4.01 QEE96 pKa = 3.56 HH97 pKa = 7.18 DD98 pKa = 4.1 EE99 pKa = 4.3 YY100 pKa = 11.89 SFDD103 pKa = 3.8 AKK105 pKa = 11.09 NNAGRR110 pKa = 11.84 VDD112 pKa = 4.57 FDD114 pKa = 4.26 LSLYY118 pKa = 10.89 SNADD122 pKa = 3.65 RR123 pKa = 11.84 ARR125 pKa = 11.84 NEE127 pKa = 3.96 YY128 pKa = 10.9 SDD130 pKa = 5.41 DD131 pKa = 3.65 LWDD134 pKa = 4.37 EE135 pKa = 4.01 TDD137 pKa = 4.35 DD138 pKa = 4.94 DD139 pKa = 4.85 YY140 pKa = 12.26 NSLFEE145 pKa = 4.99 LEE147 pKa = 4.34 QNGKK151 pKa = 9.1 LLSGLQEE158 pKa = 4.7 AGTSRR163 pKa = 11.84 YY164 pKa = 9.97 KK165 pKa = 11.04 LIGCNPDD172 pKa = 2.8 GSFTFEE178 pKa = 4.48 MDD180 pKa = 3.67 DD181 pKa = 5.14 LSNEE185 pKa = 3.92 QYY187 pKa = 11.79 NNVLQILDD195 pKa = 3.64 TLGYY199 pKa = 9.29 PVQFF203 pKa = 4.32
Molecular weight: 22.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.138
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A1U7NFM2|A0A1U7NFM2_9FIRM Uracil phosphoribosyltransferase OS=Ileibacterium valens OX=1862668 GN=upp PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.95 QPSKK9 pKa = 9.25 IKK11 pKa = 10.39 HH12 pKa = 5.45 KK13 pKa = 9.07 RR14 pKa = 11.84 THH16 pKa = 5.83 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2365
0
2365
701050
35
3587
296.4
33.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.095 ± 0.055
1.205 ± 0.02
6.129 ± 0.045
7.248 ± 0.057
4.342 ± 0.041
6.187 ± 0.049
1.89 ± 0.022
7.421 ± 0.044
6.87 ± 0.049
9.577 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.981 ± 0.03
4.911 ± 0.039
3.726 ± 0.032
3.822 ± 0.039
4.236 ± 0.047
6.75 ± 0.05
4.927 ± 0.05
6.078 ± 0.045
0.977 ± 0.016
3.627 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here