Bathycoccus prasinos
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7886 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K8EBG6|K8EBG6_9CHLO Uncharacterized protein OS=Bathycoccus prasinos OX=41875 GN=Bathy02g00280 PE=4 SV=1
MM1 pKa = 7.44 NEE3 pKa = 3.27 VALWVGAACLFGAGIGFVQGVDD25 pKa = 3.0 KK26 pKa = 11.03 ASEE29 pKa = 4.17 YY30 pKa = 10.29 FAGYY34 pKa = 10.27 LLEE37 pKa = 4.3 QSLSVDD43 pKa = 3.17 NLFVFILVFDD53 pKa = 3.94 YY54 pKa = 10.82 FKK56 pKa = 11.11 VPEE59 pKa = 4.19 STQPKK64 pKa = 7.79 VLSYY68 pKa = 10.85 GIVGAMIMRR77 pKa = 11.84 AAMILAGATAIEE89 pKa = 4.54 DD90 pKa = 4.02 FEE92 pKa = 4.52 PVLLVFAGILIFSSYY107 pKa = 10.92 KK108 pKa = 10.35 LLANNEE114 pKa = 4.07 EE115 pKa = 4.33 EE116 pKa = 4.36 EE117 pKa = 4.39 EE118 pKa = 4.13 EE119 pKa = 4.16 DD120 pKa = 4.23 LKK122 pKa = 11.58 DD123 pKa = 3.41 SAIVKK128 pKa = 8.96 FCSSMIQVSDD138 pKa = 4.37 EE139 pKa = 4.02 YY140 pKa = 11.42 DD141 pKa = 3.37 GDD143 pKa = 4.25 NFWTTAKK150 pKa = 10.84 DD151 pKa = 3.84 GVTKK155 pKa = 10.23 MATPLLLVVAVIEE168 pKa = 4.19 LSDD171 pKa = 3.51 VVFAVDD177 pKa = 4.69 SIPAVFGVTKK187 pKa = 10.57 DD188 pKa = 3.31 PFIVYY193 pKa = 8.66 TSNIFAICGLRR204 pKa = 11.84 SLFGFVSAVVSEE216 pKa = 4.27 LEE218 pKa = 3.9 YY219 pKa = 11.25 LEE221 pKa = 4.42 TSVAVVLGFIGVKK234 pKa = 9.27 MVADD238 pKa = 3.63 YY239 pKa = 11.28 AGYY242 pKa = 9.91 PMSTEE247 pKa = 3.51 ASLAVVATLLSGGVAASYY265 pKa = 9.33 LFPSAPAEE273 pKa = 4.25 VTSSVDD279 pKa = 3.23 EE280 pKa = 4.27
Molecular weight: 29.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 3.986
Dawson 3.846
Bjellqvist 4.024
Wikipedia 3.732
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.126
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.986
Patrickios 1.1
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|K8EWZ9|K8EWZ9_9CHLO Uncharacterized protein OS=Bathycoccus prasinos OX=41875 GN=Bathy06g04680 PE=4 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.24 WKK6 pKa = 10.27 KK7 pKa = 9.75 KK8 pKa = 6.4 RR9 pKa = 11.84 TRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 9.73 RR15 pKa = 11.84 KK16 pKa = 7.81 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.8 MRR21 pKa = 11.84 MRR23 pKa = 11.84 SKK25 pKa = 11.07
Molecular weight: 3.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7886
0
7886
4117150
25
16310
522.1
58.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.217 ± 0.031
1.59 ± 0.013
6.052 ± 0.024
8.439 ± 0.042
4.024 ± 0.02
6.459 ± 0.026
1.871 ± 0.013
4.217 ± 0.018
7.329 ± 0.039
7.839 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.015
4.661 ± 0.019
3.806 ± 0.019
3.167 ± 0.019
6.133 ± 0.033
8.206 ± 0.045
5.718 ± 0.03
6.359 ± 0.023
1.068 ± 0.013
2.346 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here