Beihai sobemo-like virus 7
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEA3|A0A1L3KEA3_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 7 OX=1922704 PE=4 SV=1
MM1 pKa = 8.07 DD2 pKa = 4.27 LRR4 pKa = 11.84 KK5 pKa = 10.28 VSMASPVGCSLAVAQRR21 pKa = 11.84 ILGLAKK27 pKa = 10.47 SGLGYY32 pKa = 10.79 LWLRR36 pKa = 11.84 AKK38 pKa = 10.64 NEE40 pKa = 4.56 SIVARR45 pKa = 11.84 AVGAVFAKK53 pKa = 10.41 LLSVDD58 pKa = 3.09 KK59 pKa = 10.36 EE60 pKa = 3.96 QRR62 pKa = 11.84 RR63 pKa = 11.84 KK64 pKa = 9.5 LWYY67 pKa = 10.68 SFTSYY72 pKa = 10.92 LKK74 pKa = 9.2 WAATGGSLEE83 pKa = 4.14 IKK85 pKa = 10.25 LVRR88 pKa = 11.84 LILLAVAVRR97 pKa = 11.84 KK98 pKa = 9.11 SARR101 pKa = 11.84 FARR104 pKa = 11.84 DD105 pKa = 2.79 SDD107 pKa = 3.74 RR108 pKa = 11.84 VQYY111 pKa = 10.53 CAGRR115 pKa = 11.84 LWAMCTRR122 pKa = 11.84 VPLNLRR128 pKa = 11.84 PAEE131 pKa = 4.0 EE132 pKa = 4.34 AGVIQNTSLGLIWCDD147 pKa = 3.33 PNDD150 pKa = 3.19 KK151 pKa = 9.43 RR152 pKa = 11.84 TYY154 pKa = 10.36 KK155 pKa = 9.65 LTPSVVTGEE164 pKa = 4.33 LGSIGVPQYY173 pKa = 11.72 SLTPLDD179 pKa = 3.44 ATEE182 pKa = 4.46 NLLAVHH188 pKa = 6.58 GGVDD192 pKa = 4.62 RR193 pKa = 11.84 AFTWISGVFVVCHH206 pKa = 5.73 CTDD209 pKa = 3.49 AGDD212 pKa = 3.51 MPVAHH217 pKa = 7.9 GYY219 pKa = 10.23 RR220 pKa = 11.84 SGNRR224 pKa = 11.84 AYY226 pKa = 10.91 LSRR229 pKa = 11.84 HH230 pKa = 4.49 VLEE233 pKa = 5.01 GPKK236 pKa = 10.23 AIPRR240 pKa = 11.84 TEE242 pKa = 3.78 GSLAILNPKK251 pKa = 8.98 TGDD254 pKa = 3.54 KK255 pKa = 10.53 KK256 pKa = 11.08 ALSPDD261 pKa = 3.43 FAYY264 pKa = 10.47 DD265 pKa = 3.17 LHH267 pKa = 7.68 CGSKK271 pKa = 9.89 HH272 pKa = 4.54 VPYY275 pKa = 10.43 SGSDD279 pKa = 3.28 LASIDD284 pKa = 3.64 LPGFFWAVVGVKK296 pKa = 10.23 AISEE300 pKa = 4.15 NAFRR304 pKa = 11.84 ASTEE308 pKa = 3.88 GFVFMTTFGPDD319 pKa = 2.8 GVMRR323 pKa = 11.84 TSRR326 pKa = 11.84 GSLIPQTRR334 pKa = 11.84 EE335 pKa = 3.41 DD336 pKa = 3.68 QLAGRR341 pKa = 11.84 IGYY344 pKa = 7.19 TISTEE349 pKa = 3.91 PGYY352 pKa = 10.81 SGSPVFQLVNGACKK366 pKa = 10.06 VVAIHH371 pKa = 6.03 MCGQIDD377 pKa = 3.87 EE378 pKa = 4.75 RR379 pKa = 11.84 PANFGVCVPDD389 pKa = 3.87 VIHH392 pKa = 7.22 FRR394 pKa = 11.84 RR395 pKa = 11.84 QVGATLATEE404 pKa = 4.53 LLCEE408 pKa = 4.41 SKK410 pKa = 10.64 EE411 pKa = 4.08 KK412 pKa = 11.13 SPAQYY417 pKa = 10.91 NRR419 pKa = 11.84 WDD421 pKa = 4.05 DD422 pKa = 3.66 PAFLNSIFYY431 pKa = 10.36 GQGSDD436 pKa = 5.48 DD437 pKa = 3.88 EE438 pKa = 6.32 DD439 pKa = 5.57 DD440 pKa = 3.85 YY441 pKa = 12.22 GMDD444 pKa = 3.49 EE445 pKa = 4.28 EE446 pKa = 4.45 KK447 pKa = 11.09 ARR449 pKa = 11.84 LAEE452 pKa = 4.01 QAEE455 pKa = 4.35 RR456 pKa = 11.84 EE457 pKa = 4.0 QDD459 pKa = 3.28 EE460 pKa = 4.37 RR461 pKa = 11.84 DD462 pKa = 3.21 ALLQHH467 pKa = 6.08 EE468 pKa = 4.65 RR469 pKa = 11.84 EE470 pKa = 4.02 IAGRR474 pKa = 11.84 GDD476 pKa = 4.09 DD477 pKa = 3.71 YY478 pKa = 12.03 ADD480 pKa = 3.52 FQGRR484 pKa = 11.84 DD485 pKa = 2.85 AGRR488 pKa = 11.84 RR489 pKa = 11.84 ARR491 pKa = 11.84 RR492 pKa = 11.84 AKK494 pKa = 10.71 YY495 pKa = 10.49 DD496 pKa = 3.83 DD497 pKa = 4.72 DD498 pKa = 5.42 EE499 pKa = 5.76 EE500 pKa = 5.3 DD501 pKa = 3.53 LSEE504 pKa = 4.41 SQVQCVPVHH513 pKa = 5.11 VNKK516 pKa = 9.87 WLPDD520 pKa = 3.33 TADD523 pKa = 3.73 RR524 pKa = 11.84 KK525 pKa = 10.78 SEE527 pKa = 4.02 GASLTEE533 pKa = 3.95 EE534 pKa = 4.15 QIKK537 pKa = 10.19 KK538 pKa = 9.79 QLAANAPDD546 pKa = 4.38 PDD548 pKa = 4.72 VVSTWDD554 pKa = 3.69 PKK556 pKa = 11.06 EE557 pKa = 4.16 LLASAPFARR566 pKa = 11.84 FRR568 pKa = 11.84 EE569 pKa = 4.2 YY570 pKa = 10.79 LAAGAIEE577 pKa = 4.43 WDD579 pKa = 3.64 PKK581 pKa = 8.91 EE582 pKa = 4.23 TVVSHH587 pKa = 7.11 DD588 pKa = 3.96 CNGEE592 pKa = 3.93 PVLMRR597 pKa = 11.84 VGKK600 pKa = 10.39 SEE602 pKa = 3.78 ARR604 pKa = 11.84 SSGKK608 pKa = 10.22 SSPEE612 pKa = 3.39 KK613 pKa = 10.43 KK614 pKa = 9.91 LRR616 pKa = 11.84 QQFKK620 pKa = 10.27 DD621 pKa = 3.06 ACEE624 pKa = 3.93 KK625 pKa = 10.9 FGVKK629 pKa = 8.5 MQDD632 pKa = 3.38 YY633 pKa = 10.46 IIPRR637 pKa = 11.84 DD638 pKa = 3.64 NPEE641 pKa = 4.04 AVLEE645 pKa = 4.15 SLKK648 pKa = 11.11 GQLTTHH654 pKa = 7.21 CARR657 pKa = 11.84 DD658 pKa = 3.73 LKK660 pKa = 10.34 DD661 pKa = 3.35 TPQVRR666 pKa = 11.84 EE667 pKa = 4.09 AFDD670 pKa = 4.74 KK671 pKa = 10.77 IVSSYY676 pKa = 10.22 EE677 pKa = 3.77 PCEE680 pKa = 3.76 QPYY683 pKa = 8.19 ITGGGIHH690 pKa = 6.4 YY691 pKa = 10.36 GVDD694 pKa = 3.79 AILDD698 pKa = 3.74 SLEE701 pKa = 4.16 GDD703 pKa = 4.49 KK704 pKa = 11.29 SAGWADD710 pKa = 3.59 QYY712 pKa = 11.49 RR713 pKa = 11.84 AGKK716 pKa = 9.56 KK717 pKa = 8.48 EE718 pKa = 3.51 VWKK721 pKa = 8.18 THH723 pKa = 5.33 EE724 pKa = 4.12 GRR726 pKa = 11.84 GIASYY731 pKa = 10.47 LVRR734 pKa = 11.84 CRR736 pKa = 11.84 ILLRR740 pKa = 11.84 LAWGSVAMGCMTPKK754 pKa = 10.33 QMVRR758 pKa = 11.84 EE759 pKa = 3.99 GLMDD763 pKa = 4.13 PRR765 pKa = 11.84 APFIKK770 pKa = 10.15 RR771 pKa = 11.84 EE772 pKa = 3.88 PSSVKK777 pKa = 9.88 KK778 pKa = 10.64 QKK780 pKa = 8.13 TKK782 pKa = 9.41 RR783 pKa = 11.84 WRR785 pKa = 11.84 LIWNCSVIDD794 pKa = 3.82 VLVAGITNRR803 pKa = 11.84 KK804 pKa = 7.87 QDD806 pKa = 3.42 KK807 pKa = 10.39 QDD809 pKa = 2.94 IASYY813 pKa = 10.22 QDD815 pKa = 2.98 GRR817 pKa = 11.84 AGFHH821 pKa = 5.97 CVGMGHH827 pKa = 6.89 HH828 pKa = 7.79 DD829 pKa = 4.16 DD830 pKa = 4.79 GIKK833 pKa = 10.62 AVGSVLDD840 pKa = 5.37 RR841 pKa = 11.84 IAAKK845 pKa = 10.17 CGKK848 pKa = 9.65 LSSSDD853 pKa = 3.01 ISGWDD858 pKa = 2.93 IGVQRR863 pKa = 11.84 DD864 pKa = 3.71 WIYY867 pKa = 11.51 LDD869 pKa = 2.96 GLRR872 pKa = 11.84 RR873 pKa = 11.84 IRR875 pKa = 11.84 LGPDD879 pKa = 2.82 SKK881 pKa = 10.74 IFRR884 pKa = 11.84 EE885 pKa = 4.35 LTWCEE890 pKa = 3.6 QAATSAHH897 pKa = 5.21 VLFIGGVLYY906 pKa = 10.64 QILRR910 pKa = 11.84 AGITASGIISTSAQNSFMRR929 pKa = 11.84 SLLEE933 pKa = 4.49 LIAGSEE939 pKa = 4.33 DD940 pKa = 3.78 GVSQGDD946 pKa = 4.07 DD947 pKa = 3.23 NVSSGRR953 pKa = 11.84 NNTFLEE959 pKa = 4.65 DD960 pKa = 3.2 NGVVVRR966 pKa = 11.84 DD967 pKa = 3.88 VVEE970 pKa = 4.66 SGPCGPVPFTSHH982 pKa = 6.2 EE983 pKa = 4.1 FVKK986 pKa = 10.75 RR987 pKa = 11.84 AGHH990 pKa = 5.56 WFARR994 pKa = 11.84 FMNLDD999 pKa = 3.13 KK1000 pKa = 10.77 MLARR1004 pKa = 11.84 LEE1006 pKa = 4.31 LNRR1009 pKa = 11.84 SGTGVPAQEE1018 pKa = 5.14 AIGGCLFALRR1028 pKa = 11.84 HH1029 pKa = 5.64 SAVEE1033 pKa = 4.0 MEE1035 pKa = 4.56 QFLGVCRR1042 pKa = 11.84 EE1043 pKa = 4.24 MGWNTDD1049 pKa = 3.28 VPAMPIEE1056 pKa = 4.25 TLDD1059 pKa = 3.34
Molecular weight: 116.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.108
IPC2_protein 6.122
IPC_protein 6.186
Toseland 6.351
ProMoST 6.517
Dawson 6.517
Bjellqvist 6.532
Wikipedia 6.532
Rodwell 6.504
Grimsley 6.453
Solomon 6.517
Lehninger 6.517
Nozaki 6.81
DTASelect 6.956
Thurlkill 6.971
EMBOSS 6.942
Sillero 6.912
Patrickios 4.355
IPC_peptide 6.532
IPC2_peptide 6.751
IPC2.peptide.svr19 6.741
Protein with the highest isoelectric point:
>tr|A0A1L3KEA3|A0A1L3KEA3_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 7 OX=1922704 PE=4 SV=1
MM1 pKa = 7.42 ARR3 pKa = 11.84 LPVRR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 8.56 IVKK12 pKa = 9.79 RR13 pKa = 11.84 KK14 pKa = 7.23 TNGRR18 pKa = 11.84 RR19 pKa = 11.84 STADD23 pKa = 2.91 KK24 pKa = 10.9 VQAQGTGKK32 pKa = 10.26 AVRR35 pKa = 11.84 VAFGNGNGKK44 pKa = 10.22 RR45 pKa = 11.84 NGKK48 pKa = 9.97 LSIPRR53 pKa = 11.84 LPSGCWDD60 pKa = 4.14 AFCQSHH66 pKa = 6.43 AALPRR71 pKa = 11.84 AVGPYY76 pKa = 9.18 TIVRR80 pKa = 11.84 TTRR83 pKa = 11.84 LIKK86 pKa = 8.35 TTSRR90 pKa = 11.84 WAMVGTFARR99 pKa = 11.84 FGGVAGPADD108 pKa = 4.59 GVPLWTNICCATEE121 pKa = 4.56 DD122 pKa = 3.71 GAGAIGGTAATRR134 pKa = 11.84 FYY136 pKa = 10.96 GIPLPVADD144 pKa = 4.63 TLAGPSGSGTLCPAAISVQVMANQNLQQATGQLAAAVVPVRR185 pKa = 11.84 MDD187 pKa = 2.96 MRR189 pKa = 11.84 GTTRR193 pKa = 11.84 TWSDD197 pKa = 2.86 IEE199 pKa = 4.44 SQFVSYY205 pKa = 9.64 FRR207 pKa = 11.84 PRR209 pKa = 11.84 LMSAGKK215 pKa = 9.8 LVLRR219 pKa = 11.84 GVQMDD224 pKa = 3.65 SHH226 pKa = 7.04 PLSMADD232 pKa = 3.11 VSQFRR237 pKa = 11.84 GAHH240 pKa = 5.21 EE241 pKa = 3.96 VTAALNTDD249 pKa = 3.63 GVTPAAWSLANAPYY263 pKa = 8.56 DD264 pKa = 4.03 TNGWAPMVIYY274 pKa = 10.95 NPDD277 pKa = 3.55 NASLSLLITIEE288 pKa = 3.5 WRR290 pKa = 11.84 VRR292 pKa = 11.84 FDD294 pKa = 3.67 LSNPAVSSHH303 pKa = 4.69 THH305 pKa = 6.08 HH306 pKa = 6.59 GVSSDD311 pKa = 3.74 VSWEE315 pKa = 3.82 KK316 pKa = 10.85 HH317 pKa = 4.05 IAAATRR323 pKa = 11.84 QLPGVLDD330 pKa = 3.5 IVEE333 pKa = 4.69 KK334 pKa = 10.58 VASTGMAVASMVRR347 pKa = 11.84 GG348 pKa = 3.56
Molecular weight: 36.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.414
IPC_protein 9.838
Toseland 10.394
ProMoST 10.028
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.687
Rodwell 10.774
Grimsley 10.57
Solomon 10.584
Lehninger 10.555
Nozaki 10.409
DTASelect 10.189
Thurlkill 10.409
EMBOSS 10.789
Sillero 10.452
Patrickios 10.467
IPC_peptide 10.584
IPC2_peptide 9.253
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1407
348
1059
703.5
76.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.021 ± 1.406
2.132 ± 0.336
6.326 ± 0.974
4.904 ± 1.537
3.127 ± 0.261
9.026 ± 0.221
1.99 ± 0.01
4.549 ± 0.254
4.762 ± 0.774
7.889 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.274 ± 0.428
2.772 ± 0.466
4.762 ± 0.476
3.625 ± 0.085
7.249 ± 0.107
7.392 ± 0.038
4.975 ± 1.345
7.889 ± 0.631
2.061 ± 0.115
2.274 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here