Pseudomonas phage PollyC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pollyceevirus; Pseudomonas virus PollyC

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VI14|A0A2K9VI14_9CAUD Uncharacterized protein OS=Pseudomonas phage PollyC OX=2079290 GN=PsPhPollyC_gp11 PE=4 SV=1
MM1 pKa = 7.49TEE3 pKa = 3.96QEE5 pKa = 4.43EE6 pKa = 4.24EE7 pKa = 4.34LQFLTLSQDD16 pKa = 2.85WDD18 pKa = 4.08NPCPLMWDD26 pKa = 3.62DD27 pKa = 5.0AFLTVEE33 pKa = 4.1HH34 pKa = 6.92CGLMRR39 pKa = 11.84DD40 pKa = 3.93TEE42 pKa = 4.13QDD44 pKa = 3.31GFSVMSDD51 pKa = 2.7AGFDD55 pKa = 3.38EE56 pKa = 4.62STGVDD61 pKa = 3.11TALSVQHH68 pKa = 6.67EE69 pKa = 4.69GTMLVAGYY77 pKa = 11.22LMLEE81 pKa = 4.58GNN83 pKa = 4.21

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VHY1|A0A2K9VHY1_9CAUD Uncharacterized protein OS=Pseudomonas phage PollyC OX=2079290 GN=PsPhPollyC_gp06 PE=4 SV=1
MM1 pKa = 7.47IRR3 pKa = 11.84TNNYY7 pKa = 9.22GFVAMGGGLSITNGVNRR24 pKa = 11.84EE25 pKa = 3.7VRR27 pKa = 11.84AMILEE32 pKa = 4.32RR33 pKa = 11.84EE34 pKa = 4.38AKK36 pKa = 10.15DD37 pKa = 3.24RR38 pKa = 11.84EE39 pKa = 3.95RR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 10.47GYY44 pKa = 9.92RR45 pKa = 11.84KK46 pKa = 9.97SRR48 pKa = 11.84TIRR51 pKa = 11.84PGAKK55 pKa = 8.91GDD57 pKa = 3.54TFKK60 pKa = 11.01EE61 pKa = 4.1YY62 pKa = 10.78RR63 pKa = 11.84PP64 pKa = 3.76

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12870

40

1277

262.7

28.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.756 ± 0.61

0.971 ± 0.166

6.076 ± 0.205

5.455 ± 0.269

3.364 ± 0.181

7.972 ± 0.254

2.005 ± 0.205

4.359 ± 0.23

5.058 ± 0.359

8.43 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.991 ± 0.203

4.033 ± 0.291

4.18 ± 0.188

4.779 ± 0.363

5.789 ± 0.339

5.563 ± 0.25

5.789 ± 0.365

7.195 ± 0.362

1.375 ± 0.137

2.859 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski