White spot syndrome virus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 164 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2E5Q9|A0A0S2E5Q9_9VIRU Envelope protein OS=White spot syndrome virus OX=342409 PE=4 SV=1
MM1 pKa = 7.84 AIKK4 pKa = 10.14 DD5 pKa = 3.77 YY6 pKa = 10.22 EE7 pKa = 4.32 IPDD10 pKa = 3.82 SYY12 pKa = 11.75 SKK14 pKa = 11.27 VEE16 pKa = 4.04 AEE18 pKa = 4.08 HH19 pKa = 5.64 TVEE22 pKa = 3.91 GRR24 pKa = 11.84 KK25 pKa = 6.87 MTFNIKK31 pKa = 8.82 WRR33 pKa = 11.84 GDD35 pKa = 3.65 TINNLITIIPSVTGYY50 pKa = 10.01 LASISEE56 pKa = 4.52 DD57 pKa = 3.32 ADD59 pKa = 3.55 VQAPLLLNCNNCFIEE74 pKa = 6.15 ADD76 pKa = 3.64 MSSLYY81 pKa = 9.71 MDD83 pKa = 3.94 EE84 pKa = 5.07 KK85 pKa = 10.22 KK86 pKa = 9.25 TEE88 pKa = 3.87 ASFTLNLPEE97 pKa = 5.7 IEE99 pKa = 4.4 GADD102 pKa = 3.32 ANAVYY107 pKa = 8.87 EE108 pKa = 4.0 ICIVVVV114 pKa = 3.69
Molecular weight: 12.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.114
IPC2_protein 4.24
IPC_protein 4.139
Toseland 3.961
ProMoST 4.266
Dawson 4.101
Bjellqvist 4.253
Wikipedia 3.986
Rodwell 3.973
Grimsley 3.884
Solomon 4.088
Lehninger 4.037
Nozaki 4.215
DTASelect 4.355
Thurlkill 3.986
EMBOSS 3.999
Sillero 4.253
Patrickios 2.931
IPC_peptide 4.088
IPC2_peptide 4.24
IPC2.peptide.svr19 4.159
Protein with the highest isoelectric point:
>tr|Q910R0|Q910R0_9VIRU Ribonucleoside-diphosphate reductase OS=White spot syndrome virus OX=342409 GN=RR2 PE=3 SV=1
MM1 pKa = 7.18 VARR4 pKa = 11.84 SSKK7 pKa = 8.23 TKK9 pKa = 10.2 SRR11 pKa = 11.84 RR12 pKa = 11.84 GSKK15 pKa = 9.86 KK16 pKa = 9.73 RR17 pKa = 11.84 STTAGRR23 pKa = 11.84 ISKK26 pKa = 9.98 RR27 pKa = 11.84 RR28 pKa = 11.84 SPSMKK33 pKa = 9.48 KK34 pKa = 9.83 RR35 pKa = 11.84 AGKK38 pKa = 10.17 KK39 pKa = 8.75 SSTVRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 SSKK49 pKa = 10.18 SGKK52 pKa = 9.73 KK53 pKa = 9.68 SGARR57 pKa = 11.84 KK58 pKa = 9.36 SRR60 pKa = 11.84 RR61 pKa = 3.37
Molecular weight: 6.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.389
IPC_protein 12.954
Toseland 13.13
ProMoST 13.612
Dawson 13.13
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.91
Grimsley 13.173
Solomon 13.612
Lehninger 13.525
Nozaki 13.13
DTASelect 13.115
Thurlkill 13.13
EMBOSS 13.627
Sillero 13.13
Patrickios 12.632
IPC_peptide 13.627
IPC2_peptide 12.603
IPC2.peptide.svr19 9.24
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
164
0
164
89590
61
6077
546.3
61.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.109 ± 0.202
1.786 ± 0.095
5.962 ± 0.125
7.274 ± 0.204
4.272 ± 0.093
5.633 ± 0.297
1.818 ± 0.073
5.835 ± 0.113
7.255 ± 0.146
7.779 ± 0.126
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.647 ± 0.07
6.152 ± 0.144
4.379 ± 0.184
3.023 ± 0.187
5.128 ± 0.132
8.944 ± 0.264
6.023 ± 0.121
6.308 ± 0.107
0.775 ± 0.047
2.899 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here