White spot syndrome virus 
Average proteome isoelectric point is 6.55 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 164 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A0S2E5Q9|A0A0S2E5Q9_9VIRU Envelope protein OS=White spot syndrome virus OX=342409 PE=4 SV=1MM1 pKa = 7.84  AIKK4 pKa = 10.14  DD5 pKa = 3.77  YY6 pKa = 10.22  EE7 pKa = 4.32  IPDD10 pKa = 3.82  SYY12 pKa = 11.75  SKK14 pKa = 11.27  VEE16 pKa = 4.04  AEE18 pKa = 4.08  HH19 pKa = 5.64  TVEE22 pKa = 3.91  GRR24 pKa = 11.84  KK25 pKa = 6.87  MTFNIKK31 pKa = 8.82  WRR33 pKa = 11.84  GDD35 pKa = 3.65  TINNLITIIPSVTGYY50 pKa = 10.01  LASISEE56 pKa = 4.52  DD57 pKa = 3.32  ADD59 pKa = 3.55  VQAPLLLNCNNCFIEE74 pKa = 6.15  ADD76 pKa = 3.64  MSSLYY81 pKa = 9.71  MDD83 pKa = 3.94  EE84 pKa = 5.07  KK85 pKa = 10.22  KK86 pKa = 9.25  TEE88 pKa = 3.87  ASFTLNLPEE97 pKa = 5.7  IEE99 pKa = 4.4  GADD102 pKa = 3.32  ANAVYY107 pKa = 8.87  EE108 pKa = 4.0  ICIVVVV114 pKa = 3.69  
 12.68 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.114 
IPC2_protein 4.24 
IPC_protein 4.139 
Toseland    3.961 
ProMoST     4.266 
Dawson      4.101 
Bjellqvist  4.253 
Wikipedia   3.986 
Rodwell     3.973 
Grimsley    3.884 
Solomon     4.088 
Lehninger   4.037 
Nozaki      4.215 
DTASelect   4.355 
Thurlkill   3.986 
EMBOSS      3.999 
Sillero     4.253 
Patrickios  2.931 
IPC_peptide 4.088 
IPC2_peptide  4.24 
IPC2.peptide.svr19  4.159 
 Protein with the highest isoelectric point: 
>tr|Q910R0|Q910R0_9VIRU Ribonucleoside-diphosphate reductase OS=White spot syndrome virus OX=342409 GN=RR2 PE=3 SV=1MM1 pKa = 7.18  VARR4 pKa = 11.84  SSKK7 pKa = 8.23  TKK9 pKa = 10.2  SRR11 pKa = 11.84  RR12 pKa = 11.84  GSKK15 pKa = 9.86  KK16 pKa = 9.73  RR17 pKa = 11.84  STTAGRR23 pKa = 11.84  ISKK26 pKa = 9.98  RR27 pKa = 11.84  RR28 pKa = 11.84  SPSMKK33 pKa = 9.48  KK34 pKa = 9.83  RR35 pKa = 11.84  AGKK38 pKa = 10.17  KK39 pKa = 8.75  SSTVRR44 pKa = 11.84  RR45 pKa = 11.84  RR46 pKa = 11.84  SSKK49 pKa = 10.18  SGKK52 pKa = 9.73  KK53 pKa = 9.68  SGARR57 pKa = 11.84  KK58 pKa = 9.36  SRR60 pKa = 11.84  RR61 pKa = 3.37  
 6.82 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.517 
IPC2_protein 11.389 
IPC_protein 12.954 
Toseland    13.13 
ProMoST     13.612 
Dawson      13.13 
Bjellqvist  13.115 
Wikipedia   13.598 
Rodwell     12.91 
Grimsley    13.173 
Solomon     13.612 
Lehninger   13.525 
Nozaki      13.13 
DTASelect   13.115 
Thurlkill   13.13 
EMBOSS      13.627 
Sillero     13.13 
Patrickios  12.632 
IPC_peptide 13.627 
IPC2_peptide  12.603 
IPC2.peptide.svr19  9.24 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        164 
0
164 
89590
61
6077
546.3
61.24
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        6.109 ± 0.202
1.786 ± 0.095
5.962 ± 0.125
7.274 ± 0.204
4.272 ± 0.093
5.633 ± 0.297
1.818 ± 0.073
5.835 ± 0.113
7.255 ± 0.146
7.779 ± 0.126
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.647 ± 0.07
6.152 ± 0.144
4.379 ± 0.184
3.023 ± 0.187
5.128 ± 0.132
8.944 ± 0.264
6.023 ± 0.121
6.308 ± 0.107
0.775 ± 0.047
2.899 ± 0.081
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here