Apis mellifera associated microvirus 30

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTW0|A0A3S8UTW0_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 30 OX=2494759 PE=4 SV=1
MM1 pKa = 7.13KK2 pKa = 10.54HH3 pKa = 6.55EE4 pKa = 4.32ILLTNGEE11 pKa = 4.21IKK13 pKa = 10.76LPALQKK19 pKa = 10.21RR20 pKa = 11.84ARR22 pKa = 11.84HH23 pKa = 5.29YY24 pKa = 11.79ADD26 pKa = 5.03LDD28 pKa = 3.79YY29 pKa = 11.45TNTEE33 pKa = 4.15VQQHH37 pKa = 6.03FKK39 pKa = 11.16DD40 pKa = 3.38EE41 pKa = 4.18CDD43 pKa = 3.0INNIVKK49 pKa = 10.58RR50 pKa = 11.84LGPNGLAEE58 pKa = 5.37LIKK61 pKa = 10.04IDD63 pKa = 3.67PKK65 pKa = 10.87QYY67 pKa = 11.08GDD69 pKa = 3.61FSEE72 pKa = 4.68VPTYY76 pKa = 10.44QEE78 pKa = 3.6SLLIVSKK85 pKa = 10.92AQAQFDD91 pKa = 4.4ALPADD96 pKa = 3.52LRR98 pKa = 11.84DD99 pKa = 3.74RR100 pKa = 11.84FSNDD104 pKa = 2.75PAKK107 pKa = 10.5FLEE110 pKa = 4.49FTSNKK115 pKa = 9.55EE116 pKa = 3.98NLPEE120 pKa = 4.16MIKK123 pKa = 10.71LGLATEE129 pKa = 5.61RR130 pKa = 11.84IPNPPSDD137 pKa = 5.39LEE139 pKa = 4.01ILTNEE144 pKa = 4.47LKK146 pKa = 10.53RR147 pKa = 11.84RR148 pKa = 11.84NDD150 pKa = 2.99IDD152 pKa = 3.75SKK154 pKa = 11.34KK155 pKa = 9.83EE156 pKa = 4.0SKK158 pKa = 10.27PSSSTT163 pKa = 3.2

Molecular weight: 18.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTW0|A0A3S8UTW0_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 30 OX=2494759 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 9.94CLHH5 pKa = 6.34PVRR8 pKa = 11.84YY9 pKa = 9.21YY10 pKa = 10.81RR11 pKa = 11.84SRR13 pKa = 11.84DD14 pKa = 3.32SKK16 pKa = 11.48GNLTIHH22 pKa = 6.88HH23 pKa = 6.79SPNGVNVWSNGLRR36 pKa = 11.84PCGYY40 pKa = 9.53CAHH43 pKa = 6.66CRR45 pKa = 11.84LTKK48 pKa = 9.69AQSWAIRR55 pKa = 11.84MVHH58 pKa = 6.3HH59 pKa = 6.69AQMHH63 pKa = 5.07EE64 pKa = 3.91QSSFITLTYY73 pKa = 10.54DD74 pKa = 5.29DD75 pKa = 5.59KK76 pKa = 11.64NLPQNGSLNYY86 pKa = 9.69DD87 pKa = 3.32HH88 pKa = 7.0VVNFIKK94 pKa = 10.72RR95 pKa = 11.84LRR97 pKa = 11.84KK98 pKa = 9.4RR99 pKa = 11.84LHH101 pKa = 6.47SDD103 pKa = 2.84STNISFYY110 pKa = 10.82RR111 pKa = 11.84VGEE114 pKa = 4.1YY115 pKa = 10.43GGEE118 pKa = 4.01SHH120 pKa = 6.96RR121 pKa = 11.84PHH123 pKa = 5.92YY124 pKa = 10.5HH125 pKa = 7.22LILFGYY131 pKa = 10.27NFTEE135 pKa = 4.59RR136 pKa = 11.84IKK138 pKa = 11.26YY139 pKa = 10.08KK140 pKa = 10.5GVTNEE145 pKa = 4.8RR146 pKa = 11.84IVSSKK151 pKa = 10.82KK152 pKa = 10.15DD153 pKa = 3.01DD154 pKa = 3.27RR155 pKa = 11.84TYY157 pKa = 11.2YY158 pKa = 10.47KK159 pKa = 10.7SSFLTDD165 pKa = 3.04CWGHH169 pKa = 5.79GFADD173 pKa = 4.12LGDD176 pKa = 3.6VDD178 pKa = 6.56FATCQYY184 pKa = 7.17TAKK187 pKa = 10.68YY188 pKa = 9.99VMKK191 pKa = 10.56KK192 pKa = 10.18LYY194 pKa = 10.69APTAEE199 pKa = 3.96HH200 pKa = 5.94STWLEE205 pKa = 4.09KK206 pKa = 10.57EE207 pKa = 3.86RR208 pKa = 11.84SSCSKK213 pKa = 10.25RR214 pKa = 11.84IPIGTTWIQKK224 pKa = 8.34YY225 pKa = 7.3WQDD228 pKa = 3.99VYY230 pKa = 10.79PHH232 pKa = 7.64DD233 pKa = 4.34YY234 pKa = 10.71VLHH237 pKa = 6.95DD238 pKa = 3.94GKK240 pKa = 10.77KK241 pKa = 8.19YY242 pKa = 10.29KK243 pKa = 10.19PPRR246 pKa = 11.84FYY248 pKa = 11.27DD249 pKa = 2.94EE250 pKa = 4.29WLEE253 pKa = 4.05KK254 pKa = 10.47NQPLTYY260 pKa = 10.7AKK262 pKa = 10.5VKK264 pKa = 9.98LQRR267 pKa = 11.84EE268 pKa = 4.06EE269 pKa = 4.53SEE271 pKa = 4.15NDD273 pKa = 3.36VIDD276 pKa = 4.9IRR278 pKa = 11.84DD279 pKa = 3.78LHH281 pKa = 6.39AKK283 pKa = 9.93HH284 pKa = 6.52YY285 pKa = 10.68IRR287 pKa = 11.84IQKK290 pKa = 9.3QEE292 pKa = 3.56QFKK295 pKa = 10.89RR296 pKa = 11.84DD297 pKa = 4.4GIPHH301 pKa = 7.76DD302 pKa = 3.91LTLDD306 pKa = 3.95KK307 pKa = 10.89ILLEE311 pKa = 4.55RR312 pKa = 11.84KK313 pKa = 9.68LSTLHH318 pKa = 7.46DD319 pKa = 3.49IAKK322 pKa = 10.69GNLL325 pKa = 3.23

Molecular weight: 38.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1377

101

522

275.4

30.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.424 ± 2.232

0.944 ± 0.358

5.882 ± 0.483

4.938 ± 0.705

3.631 ± 0.527

6.245 ± 0.794

2.832 ± 0.905

5.374 ± 0.395

6.245 ± 1.501

7.553 ± 0.772

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.033 ± 0.305

5.737 ± 0.557

5.374 ± 1.003

4.575 ± 0.494

5.664 ± 0.341

7.262 ± 0.31

6.609 ± 0.655

5.011 ± 1.022

1.38 ± 0.268

4.285 ± 0.689
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski