Escherichia phage vB_EcoP_SP7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5K352|A0A7D5K352_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_SP7 OX=2750854 GN=SP7_0021 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.32AFNEE21 pKa = 4.57SIQHH25 pKa = 6.35DD26 pKa = 4.78EE27 pKa = 3.89IAEE30 pKa = 4.18GDD32 pKa = 4.01DD33 pKa = 3.79YY34 pKa = 11.88SDD36 pKa = 3.92ALHH39 pKa = 6.18EE40 pKa = 4.53VVEE43 pKa = 4.77GMVPHH48 pKa = 6.95YY49 pKa = 9.42YY50 pKa = 10.35HH51 pKa = 7.68EE52 pKa = 4.82IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.3DD68 pKa = 4.36SGLMPEE74 pKa = 4.55TKK76 pKa = 10.2DD77 pKa = 3.18VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 10.8NDD93 pKa = 3.95VSNSSDD99 pKa = 3.67VVWFEE104 pKa = 5.15DD105 pKa = 4.0EE106 pKa = 4.48EE107 pKa = 4.62NDD109 pKa = 5.35EE110 pKa = 4.33EE111 pKa = 4.89DD112 pKa = 3.76CC113 pKa = 5.58

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5JNH9|A0A7D5JNH9_9CAUD DNA primase/helicase OS=Escherichia phage vB_EcoP_SP7 OX=2750854 GN=SP7_0020 PE=4 SV=1
MM1 pKa = 7.31SWILLVIFGYY11 pKa = 10.83GLIAYY16 pKa = 10.04VLMRR20 pKa = 11.84DD21 pKa = 3.04INKK24 pKa = 9.28ARR26 pKa = 11.84KK27 pKa = 8.51VYY29 pKa = 9.93KK30 pKa = 10.34FNYY33 pKa = 8.71VSLGRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FTRR50 pKa = 11.84NLANVWDD57 pKa = 4.02IATLGSKK64 pKa = 10.07LL65 pKa = 3.58

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12045

37

1295

227.3

25.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.535 ± 0.45

0.971 ± 0.149

6.326 ± 0.25

6.758 ± 0.351

3.67 ± 0.147

8.045 ± 0.426

1.694 ± 0.176

5.023 ± 0.205

6.509 ± 0.398

8.037 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.873 ± 0.161

4.508 ± 0.247

3.62 ± 0.223

3.877 ± 0.379

5.471 ± 0.245

6.343 ± 0.37

5.654 ± 0.183

7.165 ± 0.263

1.436 ± 0.147

3.487 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski