Clostridium sp. CAG:264

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5KGT4|R5KGT4_9CLOT MATE domain protein OS=Clostridium sp. CAG:264 OX=1262786 GN=BN572_01375 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.18RR3 pKa = 11.84GHH5 pKa = 6.98CILAAGLCILAGLVSGCGDD24 pKa = 3.27KK25 pKa = 10.74KK26 pKa = 10.52QQTSEE31 pKa = 4.38EE32 pKa = 4.11ITTAEE37 pKa = 4.54DD38 pKa = 3.33GTVAVEE44 pKa = 4.74VLDD47 pKa = 3.94NDD49 pKa = 4.65QIALVYY55 pKa = 10.43NGYY58 pKa = 10.44DD59 pKa = 3.67PEE61 pKa = 4.34VHH63 pKa = 6.78GMMFTITNKK72 pKa = 10.07SDD74 pKa = 3.27DD75 pKa = 3.6QMVNVYY81 pKa = 10.16FSDD84 pKa = 3.82VVVDD88 pKa = 5.18GEE90 pKa = 4.54TLQPMFGLDD99 pKa = 3.36VEE101 pKa = 4.69AQTSEE106 pKa = 4.2DD107 pKa = 3.83QICLVDD113 pKa = 4.85CDD115 pKa = 4.31AAPAALTGIVCVDD128 pKa = 3.46DD129 pKa = 3.71MEE131 pKa = 6.43GYY133 pKa = 9.08TIGTYY138 pKa = 10.24EE139 pKa = 4.28IDD141 pKa = 5.2DD142 pKa = 3.75VALSNEE148 pKa = 3.82KK149 pKa = 10.61DD150 pKa = 3.35DD151 pKa = 3.94TKK153 pKa = 11.13EE154 pKa = 3.86YY155 pKa = 9.31TVKK158 pKa = 10.58EE159 pKa = 4.09GAVTILDD166 pKa = 3.66NDD168 pKa = 3.93KK169 pKa = 10.0VTVSYY174 pKa = 10.7IGIEE178 pKa = 3.99NDD180 pKa = 3.11SDD182 pKa = 4.22GDD184 pKa = 3.83DD185 pKa = 4.05AEE187 pKa = 4.23QVLAFEE193 pKa = 5.44VYY195 pKa = 10.58NKK197 pKa = 9.02TDD199 pKa = 3.2KK200 pKa = 10.9KK201 pKa = 10.47IQATFDD207 pKa = 3.5TVLVNGKK214 pKa = 7.98EE215 pKa = 4.0EE216 pKa = 4.41EE217 pKa = 3.87ISYY220 pKa = 10.7VADD223 pKa = 3.86AMGHH227 pKa = 5.56TYY229 pKa = 9.2ATLFCDD235 pKa = 3.74VSDD238 pKa = 4.12VKK240 pKa = 11.57AMTTLTGEE248 pKa = 4.01FTVYY252 pKa = 10.88DD253 pKa = 4.47EE254 pKa = 5.35DD255 pKa = 5.53DD256 pKa = 3.9EE257 pKa = 6.27LIGSYY262 pKa = 10.28SIEE265 pKa = 4.1KK266 pKa = 10.29AALKK270 pKa = 10.48

Molecular weight: 29.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5K4X7|R5K4X7_9CLOT DNA helicase OS=Clostridium sp. CAG:264 OX=1262786 GN=BN572_00987 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.89VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2136

0

2136

706887

30

2768

330.9

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.411 ± 0.053

1.472 ± 0.022

6.273 ± 0.049

7.237 ± 0.063

3.931 ± 0.046

6.942 ± 0.058

1.676 ± 0.024

7.832 ± 0.05

7.265 ± 0.053

8.341 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.132 ± 0.033

4.627 ± 0.037

3.027 ± 0.029

2.926 ± 0.023

4.02 ± 0.037

5.788 ± 0.048

5.765 ± 0.061

7.046 ± 0.039

0.783 ± 0.017

4.507 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski