Luteococcus japonicus LSP_Lj1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R4J012|A0A1R4J012_9ACTN SSS sodium solute transporter superfamily OS=Luteococcus japonicus LSP_Lj1 OX=1255658 GN=FM114_04595 PE=3 SV=1
MM1 pKa = 7.14 VCHH4 pKa = 7.06 GDD6 pKa = 3.13 ACNPNFLIGDD16 pKa = 4.13 DD17 pKa = 4.43 GEE19 pKa = 4.42 VSGFVDD25 pKa = 4.3 LGRR28 pKa = 11.84 TGVADD33 pKa = 4.06 RR34 pKa = 11.84 HH35 pKa = 6.45 ADD37 pKa = 3.57 LAPAVLSLGWNFGDD51 pKa = 3.36 GWTDD55 pKa = 3.25 TFLTAYY61 pKa = 9.85 CADD64 pKa = 3.8 GLTVDD69 pKa = 4.41 RR70 pKa = 11.84 DD71 pKa = 3.64 LLEE74 pKa = 4.3 LYY76 pKa = 9.94 IRR78 pKa = 11.84 LWNAEE83 pKa = 3.78
Molecular weight: 9.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.872
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 1.939
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|A0A1R4J3F5|A0A1R4J3F5_9ACTN Alkyl hydroperoxide reductase protein F OS=Luteococcus japonicus LSP_Lj1 OX=1255658 GN=FM114_05330 PE=3 SV=1
MM1 pKa = 7.1 HH2 pKa = 7.01 TRR4 pKa = 11.84 HH5 pKa = 6.38 HH6 pKa = 6.35 GASFTRR12 pKa = 11.84 RR13 pKa = 11.84 QHH15 pKa = 7.23 SIGKK19 pKa = 8.42 PNPLVRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 VGPVTLGWRR36 pKa = 11.84 AA37 pKa = 2.98
Molecular weight: 4.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3244
0
3244
992894
37
2336
306.1
33.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.24 ± 0.051
0.889 ± 0.015
5.91 ± 0.037
5.745 ± 0.045
2.87 ± 0.028
8.922 ± 0.037
2.377 ± 0.022
3.941 ± 0.03
2.699 ± 0.044
10.292 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.393 ± 0.018
2.183 ± 0.025
5.355 ± 0.033
3.513 ± 0.026
7.106 ± 0.044
5.443 ± 0.032
6.011 ± 0.036
8.696 ± 0.039
1.604 ± 0.023
1.809 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here