Penicillium flavigenum
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10875 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6TPE9|A0A1V6TPE9_9EURO Uncharacterized protein OS=Penicillium flavigenum OX=254877 GN=PENFLA_c005G04698 PE=4 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 TQQLLPICGLLLASVAIASKK22 pKa = 10.51 LDD24 pKa = 3.58 HH25 pKa = 7.67 DD26 pKa = 5.13 DD27 pKa = 3.58 VPSRR31 pKa = 11.84 CWPACGPVVGIANSCDD47 pKa = 3.12 ARR49 pKa = 11.84 HH50 pKa = 5.93 EE51 pKa = 4.22 RR52 pKa = 11.84 DD53 pKa = 3.38 SAEE56 pKa = 4.0 IQCICDD62 pKa = 2.94 WDD64 pKa = 3.85 AAKK67 pKa = 8.95 TQIPLCAACITQYY80 pKa = 9.22 QTDD83 pKa = 3.77 RR84 pKa = 11.84 RR85 pKa = 11.84 NHH87 pKa = 5.34 NTTHH91 pKa = 7.06 HH92 pKa = 6.84 DD93 pKa = 3.6 HH94 pKa = 7.89 DD95 pKa = 6.29 DD96 pKa = 3.95 NDD98 pKa = 5.61 DD99 pKa = 4.47 DD100 pKa = 6.95 DD101 pKa = 6.58 DD102 pKa = 4.95 DD103 pKa = 6.13 HH104 pKa = 9.59 DD105 pKa = 5.0 YY106 pKa = 11.92 DD107 pKa = 4.69 NDD109 pKa = 3.57 NDD111 pKa = 4.15 NDD113 pKa = 3.96 IDD115 pKa = 5.47 DD116 pKa = 5.56 DD117 pKa = 4.89 DD118 pKa = 6.11 NEE120 pKa = 4.39 ALDD123 pKa = 4.42 LVRR126 pKa = 11.84 SCKK129 pKa = 9.64 LTTTTYY135 pKa = 10.94 NSAAATTVSTSTSTAGANTATTTDD159 pKa = 3.19 SSNAATGTDD168 pKa = 3.29 SAGAGGTNSQDD179 pKa = 3.2 STGAAGASAGSASSPTPTPDD199 pKa = 2.9 AAAGILAPGAASMAGVMGLVALAWLL224 pKa = 4.2
Molecular weight: 23.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.859
IPC_protein 3.91
Toseland 3.668
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.935
Rodwell 3.732
Grimsley 3.567
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.406
Thurlkill 3.745
EMBOSS 3.935
Sillero 4.05
Patrickios 1.24
IPC_peptide 3.923
IPC2_peptide 4.012
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A1V6S7P2|A0A1V6S7P2_9EURO Uncharacterized protein (Fragment) OS=Penicillium flavigenum OX=254877 GN=PENFLA_c097G10561 PE=4 SV=1
MM1 pKa = 7.5 SLRR4 pKa = 11.84 RR5 pKa = 11.84 LLVSQMTLRR14 pKa = 11.84 RR15 pKa = 11.84 LLVSQLSLRR24 pKa = 11.84 RR25 pKa = 11.84 LLVSQMSLRR34 pKa = 11.84 RR35 pKa = 11.84 LLLSQMSLRR44 pKa = 11.84 RR45 pKa = 11.84 LLLSQMSLRR54 pKa = 11.84 RR55 pKa = 11.84 LLLSQMSLRR64 pKa = 11.84 RR65 pKa = 11.84 LLVSQMSLRR74 pKa = 11.84 RR75 pKa = 11.84 LLLSQMSLRR84 pKa = 11.84 RR85 pKa = 11.84 LLVSQLSLRR94 pKa = 11.84 RR95 pKa = 11.84 LLLTQRR101 pKa = 11.84 SPAGLTCHH109 pKa = 6.58 HH110 pKa = 6.59 SVPLRR115 pKa = 11.84 SSLGG119 pKa = 3.46
Molecular weight: 13.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.223
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.22
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10875
0
10875
5176197
10
6336
476.0
52.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.423 ± 0.019
1.269 ± 0.009
5.669 ± 0.017
6.12 ± 0.024
3.801 ± 0.015
6.779 ± 0.022
2.441 ± 0.01
5.074 ± 0.017
4.62 ± 0.019
8.971 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.008
3.738 ± 0.012
6.048 ± 0.027
4.072 ± 0.017
5.966 ± 0.022
8.332 ± 0.026
5.969 ± 0.017
6.157 ± 0.017
1.495 ± 0.01
2.798 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here