Sugarcane yellow leaf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9WI47|Q9WI47_9LUTE Movement protein OS=Sugarcane yellow leaf virus OX=94290 GN=ORF4 PE=2 SV=1
MM1 pKa = 7.91SEE3 pKa = 4.0DD4 pKa = 3.35ALTVVDD10 pKa = 6.27RR11 pKa = 11.84LGQWSWSGLPQDD23 pKa = 4.29LDD25 pKa = 3.7EE26 pKa = 5.86YY27 pKa = 11.52DD28 pKa = 4.31DD29 pKa = 4.13VEE31 pKa = 4.47HH32 pKa = 7.27VLEE35 pKa = 4.33EE36 pKa = 4.28TLCEE40 pKa = 4.08DD41 pKa = 4.33RR42 pKa = 11.84EE43 pKa = 4.2EE44 pKa = 4.33EE45 pKa = 4.08ATGMFSLSRR54 pKa = 11.84LTISKK59 pKa = 7.72PTQPGSSNSDD69 pKa = 2.74RR70 pKa = 11.84TYY72 pKa = 11.49LSTQRR77 pKa = 11.84STMAYY82 pKa = 9.43SKK84 pKa = 9.19PTMSIKK90 pKa = 10.6SQVSLFSITHH100 pKa = 6.69APPTQLQVQSHH111 pKa = 6.52LKK113 pKa = 9.14WIHH116 pKa = 6.27PAPKK120 pKa = 9.34QQQAPRR126 pKa = 11.84LLASPSRR133 pKa = 11.84GTPRR137 pKa = 11.84KK138 pKa = 9.38SSRR141 pKa = 11.84PPSSGGKK148 pKa = 9.51ISS150 pKa = 3.16

Molecular weight: 16.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9QQN8|Q9QQN8_9LUTE RNA-directed RNA polymerase (Fragment) OS=Sugarcane yellow leaf virus OX=94290 PE=4 SV=1
MM1 pKa = 6.87NTGANRR7 pKa = 11.84SRR9 pKa = 11.84RR10 pKa = 11.84NVRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ANRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84QTRR23 pKa = 11.84PVVVVRR29 pKa = 11.84APPGPRR35 pKa = 11.84RR36 pKa = 11.84VRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84ARR43 pKa = 11.84VGGNAVRR50 pKa = 11.84GPGGRR55 pKa = 11.84SNRR58 pKa = 11.84DD59 pKa = 3.02VLTFTVDD66 pKa = 3.38DD67 pKa = 4.74LKK69 pKa = 11.69ANSTGILKK77 pKa = 10.11FGPNLSQYY85 pKa = 11.29AAFNNGLLKK94 pKa = 10.37AYY96 pKa = 9.79HH97 pKa = 6.59EE98 pKa = 4.35YY99 pKa = 10.72KK100 pKa = 9.38ITSLTIQYY108 pKa = 9.67NSCSSDD114 pKa = 3.1ATPGAIALEE123 pKa = 4.33VDD125 pKa = 4.38TSCSQTTTGSKK136 pKa = 8.38ITSFPVKK143 pKa = 10.24RR144 pKa = 11.84NAKK147 pKa = 9.58KK148 pKa = 10.49VFPAPFIRR156 pKa = 11.84GKK158 pKa = 10.79DD159 pKa = 3.78FMTTSADD166 pKa = 4.07QFWLLYY172 pKa = 9.95KK173 pKa = 11.05GNGDD177 pKa = 3.49SSLAGQFVCRR187 pKa = 11.84FEE189 pKa = 6.15CLFQNPKK196 pKa = 10.36

Molecular weight: 21.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2303

150

650

383.8

42.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.121 ± 0.799

1.433 ± 0.282

4.733 ± 0.247

5.688 ± 0.669

3.821 ± 0.407

6.166 ± 0.504

2.301 ± 0.429

3.865 ± 0.485

5.732 ± 0.572

8.684 ± 0.677

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.911 ± 0.358

4.472 ± 0.418

7.338 ± 1.088

4.038 ± 0.364

6.34 ± 1.004

8.988 ± 0.869

6.036 ± 0.576

6.426 ± 0.29

1.824 ± 0.304

3.04 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski