Amino acid dipepetide frequency for Mycobacterium phage Kalpine

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.219AlaAla: 11.219 ± 1.148
0.552AlaCys: 0.552 ± 0.172
4.966AlaAsp: 4.966 ± 0.555
7.357AlaGlu: 7.357 ± 0.627
3.556AlaPhe: 3.556 ± 0.467
9.012AlaGly: 9.012 ± 0.954
1.594AlaHis: 1.594 ± 0.304
3.985AlaIle: 3.985 ± 0.517
5.027AlaLys: 5.027 ± 0.503
8.705AlaLeu: 8.705 ± 0.96
2.33AlaMet: 2.33 ± 0.353
3.556AlaAsn: 3.556 ± 0.444
4.843AlaPro: 4.843 ± 0.641
4.107AlaGln: 4.107 ± 0.605
6.56AlaArg: 6.56 ± 0.635
4.169AlaSer: 4.169 ± 0.473
5.579AlaThr: 5.579 ± 0.652
7.05AlaVal: 7.05 ± 0.609
1.778AlaTrp: 1.778 ± 0.346
2.943AlaTyr: 2.943 ± 0.496
0.0AlaXaa: 0.0 ± 0.0
Cys
0.429CysAla: 0.429 ± 0.133
0.061CysCys: 0.061 ± 0.066
0.613CysAsp: 0.613 ± 0.205
0.736CysGlu: 0.736 ± 0.198
0.368CysPhe: 0.368 ± 0.171
0.92CysGly: 0.92 ± 0.286
0.184CysHis: 0.184 ± 0.115
0.245CysIle: 0.245 ± 0.141
0.368CysLys: 0.368 ± 0.135
0.613CysLeu: 0.613 ± 0.224
0.184CysMet: 0.184 ± 0.116
0.429CysAsn: 0.429 ± 0.137
0.429CysPro: 0.429 ± 0.188
0.123CysGln: 0.123 ± 0.079
0.552CysArg: 0.552 ± 0.197
0.429CysSer: 0.429 ± 0.161
0.736CysThr: 0.736 ± 0.196
0.674CysVal: 0.674 ± 0.223
0.368CysTrp: 0.368 ± 0.176
0.429CysTyr: 0.429 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
6.314AspAla: 6.314 ± 0.598
0.736AspCys: 0.736 ± 0.225
4.23AspAsp: 4.23 ± 0.56
4.659AspGlu: 4.659 ± 0.589
2.759AspPhe: 2.759 ± 0.509
5.579AspGly: 5.579 ± 0.612
1.471AspHis: 1.471 ± 0.311
3.433AspIle: 3.433 ± 0.414
2.207AspLys: 2.207 ± 0.357
5.272AspLeu: 5.272 ± 0.659
1.717AspMet: 1.717 ± 0.341
1.287AspAsn: 1.287 ± 0.298
4.475AspPro: 4.475 ± 0.672
1.717AspGln: 1.717 ± 0.296
3.065AspArg: 3.065 ± 0.497
2.82AspSer: 2.82 ± 0.435
3.372AspThr: 3.372 ± 0.534
4.046AspVal: 4.046 ± 0.46
1.349AspTrp: 1.349 ± 0.304
2.391AspTyr: 2.391 ± 0.317
0.0AspXaa: 0.0 ± 0.0
Glu
7.663GluAla: 7.663 ± 0.87
0.0GluCys: 0.0 ± 0.0
4.843GluAsp: 4.843 ± 0.646
5.272GluGlu: 5.272 ± 0.66
3.249GluPhe: 3.249 ± 0.395
5.211GluGly: 5.211 ± 0.636
1.471GluHis: 1.471 ± 0.298
3.31GluIle: 3.31 ± 0.444
2.513GluLys: 2.513 ± 0.34
7.847GluLeu: 7.847 ± 0.736
2.33GluMet: 2.33 ± 0.352
2.146GluAsn: 2.146 ± 0.363
2.82GluPro: 2.82 ± 0.482
2.084GluGln: 2.084 ± 0.233
4.659GluArg: 4.659 ± 0.565
2.759GluSer: 2.759 ± 0.451
3.862GluThr: 3.862 ± 0.548
4.659GluVal: 4.659 ± 0.663
1.471GluTrp: 1.471 ± 0.305
1.471GluTyr: 1.471 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
2.943PheAla: 2.943 ± 0.409
0.184PheCys: 0.184 ± 0.098
2.575PheAsp: 2.575 ± 0.517
2.82PheGlu: 2.82 ± 0.403
0.92PhePhe: 0.92 ± 0.268
3.556PheGly: 3.556 ± 0.482
0.797PheHis: 0.797 ± 0.223
1.349PheIle: 1.349 ± 0.234
1.349PheLys: 1.349 ± 0.344
2.759PheLeu: 2.759 ± 0.452
0.429PheMet: 0.429 ± 0.167
1.533PheAsn: 1.533 ± 0.291
1.839PhePro: 1.839 ± 0.349
1.41PheGln: 1.41 ± 0.35
1.9PheArg: 1.9 ± 0.319
2.268PheSer: 2.268 ± 0.335
2.146PheThr: 2.146 ± 0.287
2.452PheVal: 2.452 ± 0.394
0.552PheTrp: 0.552 ± 0.157
0.858PheTyr: 0.858 ± 0.211
0.0PheXaa: 0.0 ± 0.0
Gly
7.418GlyAla: 7.418 ± 0.921
0.858GlyCys: 0.858 ± 0.229
6.437GlyAsp: 6.437 ± 0.747
5.701GlyGlu: 5.701 ± 0.509
3.372GlyPhe: 3.372 ± 0.43
7.786GlyGly: 7.786 ± 1.624
1.778GlyHis: 1.778 ± 0.312
3.923GlyIle: 3.923 ± 0.601
3.678GlyLys: 3.678 ± 0.521
7.295GlyLeu: 7.295 ± 0.812
1.9GlyMet: 1.9 ± 0.299
3.127GlyAsn: 3.127 ± 0.47
5.027GlyPro: 5.027 ± 1.241
3.31GlyGln: 3.31 ± 0.487
3.923GlyArg: 3.923 ± 0.435
3.862GlySer: 3.862 ± 0.503
5.701GlyThr: 5.701 ± 0.771
6.744GlyVal: 6.744 ± 0.725
1.165GlyTrp: 1.165 ± 0.266
2.636GlyTyr: 2.636 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
1.655HisAla: 1.655 ± 0.26
0.307HisCys: 0.307 ± 0.132
1.103HisAsp: 1.103 ± 0.25
1.287HisGlu: 1.287 ± 0.298
0.552HisPhe: 0.552 ± 0.193
1.9HisGly: 1.9 ± 0.381
0.49HisHis: 0.49 ± 0.154
0.797HisIle: 0.797 ± 0.278
0.981HisLys: 0.981 ± 0.266
1.594HisLeu: 1.594 ± 0.317
0.245HisMet: 0.245 ± 0.126
0.613HisAsn: 0.613 ± 0.197
1.226HisPro: 1.226 ± 0.247
0.797HisGln: 0.797 ± 0.198
1.349HisArg: 1.349 ± 0.345
0.736HisSer: 0.736 ± 0.228
1.103HisThr: 1.103 ± 0.224
1.349HisVal: 1.349 ± 0.283
0.49HisTrp: 0.49 ± 0.195
0.552HisTyr: 0.552 ± 0.263
0.0HisXaa: 0.0 ± 0.0
Ile
6.008IleAla: 6.008 ± 0.606
0.736IleCys: 0.736 ± 0.202
3.678IleAsp: 3.678 ± 0.456
4.291IleGlu: 4.291 ± 0.496
1.226IlePhe: 1.226 ± 0.295
4.475IleGly: 4.475 ± 0.588
0.92IleHis: 0.92 ± 0.203
1.839IleIle: 1.839 ± 0.308
2.023IleLys: 2.023 ± 0.435
3.617IleLeu: 3.617 ± 0.403
0.613IleMet: 0.613 ± 0.19
1.962IleAsn: 1.962 ± 0.396
3.127IlePro: 3.127 ± 0.455
1.287IleGln: 1.287 ± 0.321
3.004IleArg: 3.004 ± 0.394
2.636IleSer: 2.636 ± 0.397
3.127IleThr: 3.127 ± 0.372
2.759IleVal: 2.759 ± 0.395
0.613IleTrp: 0.613 ± 0.177
0.797IleTyr: 0.797 ± 0.224
0.0IleXaa: 0.0 ± 0.0
Lys
5.088LysAla: 5.088 ± 0.638
0.368LysCys: 0.368 ± 0.182
2.023LysAsp: 2.023 ± 0.346
2.207LysGlu: 2.207 ± 0.362
1.042LysPhe: 1.042 ± 0.242
3.985LysGly: 3.985 ± 0.685
0.552LysHis: 0.552 ± 0.198
2.023LysIle: 2.023 ± 0.332
2.943LysLys: 2.943 ± 0.582
4.107LysLeu: 4.107 ± 0.511
1.103LysMet: 1.103 ± 0.3
1.471LysAsn: 1.471 ± 0.277
2.759LysPro: 2.759 ± 0.493
1.471LysGln: 1.471 ± 0.293
3.065LysArg: 3.065 ± 0.523
1.962LysSer: 1.962 ± 0.264
3.127LysThr: 3.127 ± 0.42
3.801LysVal: 3.801 ± 0.423
0.674LysTrp: 0.674 ± 0.211
1.287LysTyr: 1.287 ± 0.25
0.0LysXaa: 0.0 ± 0.0
Leu
9.196LeuAla: 9.196 ± 0.889
0.736LeuCys: 0.736 ± 0.213
5.272LeuAsp: 5.272 ± 0.605
4.782LeuGlu: 4.782 ± 0.661
2.82LeuPhe: 2.82 ± 0.34
6.069LeuGly: 6.069 ± 0.822
1.655LeuHis: 1.655 ± 0.354
4.537LeuIle: 4.537 ± 0.47
3.74LeuLys: 3.74 ± 0.48
5.701LeuLeu: 5.701 ± 0.663
2.575LeuMet: 2.575 ± 0.54
2.207LeuAsn: 2.207 ± 0.489
4.904LeuPro: 4.904 ± 0.433
2.636LeuGln: 2.636 ± 0.488
6.437LeuArg: 6.437 ± 0.713
5.272LeuSer: 5.272 ± 0.499
5.456LeuThr: 5.456 ± 0.625
4.353LeuVal: 4.353 ± 0.58
1.839LeuTrp: 1.839 ± 0.294
2.452LeuTyr: 2.452 ± 0.433
0.0LeuXaa: 0.0 ± 0.0
Met
3.556MetAla: 3.556 ± 0.464
0.061MetCys: 0.061 ± 0.053
0.92MetAsp: 0.92 ± 0.24
1.103MetGlu: 1.103 ± 0.227
0.736MetPhe: 0.736 ± 0.203
1.778MetGly: 1.778 ± 0.329
0.49MetHis: 0.49 ± 0.173
1.41MetIle: 1.41 ± 0.303
1.41MetLys: 1.41 ± 0.295
1.349MetLeu: 1.349 ± 0.27
0.429MetMet: 0.429 ± 0.172
0.552MetAsn: 0.552 ± 0.191
1.349MetPro: 1.349 ± 0.3
1.042MetGln: 1.042 ± 0.228
1.778MetArg: 1.778 ± 0.32
2.33MetSer: 2.33 ± 0.409
2.33MetThr: 2.33 ± 0.351
1.165MetVal: 1.165 ± 0.21
0.245MetTrp: 0.245 ± 0.119
0.736MetTyr: 0.736 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
2.82AsnAla: 2.82 ± 0.518
0.613AsnCys: 0.613 ± 0.195
1.533AsnAsp: 1.533 ± 0.276
2.513AsnGlu: 2.513 ± 0.451
0.613AsnPhe: 0.613 ± 0.239
4.291AsnGly: 4.291 ± 0.595
0.981AsnHis: 0.981 ± 0.247
1.042AsnIle: 1.042 ± 0.258
1.287AsnLys: 1.287 ± 0.274
3.065AsnLeu: 3.065 ± 0.408
0.736AsnMet: 0.736 ± 0.2
0.858AsnAsn: 0.858 ± 0.211
2.268AsnPro: 2.268 ± 0.368
0.981AsnGln: 0.981 ± 0.296
2.023AsnArg: 2.023 ± 0.42
1.287AsnSer: 1.287 ± 0.255
1.471AsnThr: 1.471 ± 0.327
2.391AsnVal: 2.391 ± 0.392
0.858AsnTrp: 0.858 ± 0.212
0.981AsnTyr: 0.981 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
5.333ProAla: 5.333 ± 0.557
0.368ProCys: 0.368 ± 0.171
3.74ProAsp: 3.74 ± 0.507
4.904ProGlu: 4.904 ± 0.495
1.655ProPhe: 1.655 ± 0.391
4.598ProGly: 4.598 ± 0.463
1.042ProHis: 1.042 ± 0.237
3.065ProIle: 3.065 ± 0.374
2.391ProLys: 2.391 ± 0.528
3.127ProLeu: 3.127 ± 0.509
1.349ProMet: 1.349 ± 0.306
2.268ProAsn: 2.268 ± 0.402
2.759ProPro: 2.759 ± 0.414
2.146ProGln: 2.146 ± 0.507
3.923ProArg: 3.923 ± 0.586
3.31ProSer: 3.31 ± 0.464
3.494ProThr: 3.494 ± 0.434
4.169ProVal: 4.169 ± 0.46
0.92ProTrp: 0.92 ± 0.299
1.41ProTyr: 1.41 ± 0.291
0.0ProXaa: 0.0 ± 0.0
Gln
4.046GlnAla: 4.046 ± 0.521
0.368GlnCys: 0.368 ± 0.159
1.165GlnAsp: 1.165 ± 0.244
1.778GlnGlu: 1.778 ± 0.335
1.533GlnPhe: 1.533 ± 0.359
3.372GlnGly: 3.372 ± 0.628
0.674GlnHis: 0.674 ± 0.215
2.391GlnIle: 2.391 ± 0.306
1.533GlnLys: 1.533 ± 0.308
3.065GlnLeu: 3.065 ± 0.602
1.042GlnMet: 1.042 ± 0.251
0.92GlnAsn: 0.92 ± 0.25
1.41GlnPro: 1.41 ± 0.29
1.9GlnGln: 1.9 ± 0.406
2.575GlnArg: 2.575 ± 0.387
1.839GlnSer: 1.839 ± 0.351
1.778GlnThr: 1.778 ± 0.331
2.207GlnVal: 2.207 ± 0.378
0.858GlnTrp: 0.858 ± 0.235
1.103GlnTyr: 1.103 ± 0.214
0.0GlnXaa: 0.0 ± 0.0
Arg
4.782ArgAla: 4.782 ± 0.658
0.92ArgCys: 0.92 ± 0.238
4.107ArgAsp: 4.107 ± 0.572
5.456ArgGlu: 5.456 ± 0.596
2.759ArgPhe: 2.759 ± 0.447
4.291ArgGly: 4.291 ± 0.608
1.41ArgHis: 1.41 ± 0.335
3.74ArgIle: 3.74 ± 0.554
3.678ArgLys: 3.678 ± 0.531
5.333ArgLeu: 5.333 ± 0.538
2.146ArgMet: 2.146 ± 0.424
1.9ArgAsn: 1.9 ± 0.365
2.759ArgPro: 2.759 ± 0.347
2.084ArgGln: 2.084 ± 0.336
5.456ArgArg: 5.456 ± 0.613
2.697ArgSer: 2.697 ± 0.387
2.759ArgThr: 2.759 ± 0.393
4.966ArgVal: 4.966 ± 0.59
1.103ArgTrp: 1.103 ± 0.278
2.268ArgTyr: 2.268 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
3.862SerAla: 3.862 ± 0.538
0.49SerCys: 0.49 ± 0.162
3.494SerAsp: 3.494 ± 0.468
3.74SerGlu: 3.74 ± 0.431
1.839SerPhe: 1.839 ± 0.327
4.843SerGly: 4.843 ± 0.497
0.613SerHis: 0.613 ± 0.211
2.575SerIle: 2.575 ± 0.327
2.391SerLys: 2.391 ± 0.524
4.291SerLeu: 4.291 ± 0.526
1.41SerMet: 1.41 ± 0.278
1.226SerAsn: 1.226 ± 0.24
3.188SerPro: 3.188 ± 0.438
1.655SerGln: 1.655 ± 0.334
3.494SerArg: 3.494 ± 0.469
2.881SerSer: 2.881 ± 0.578
3.004SerThr: 3.004 ± 0.396
3.862SerVal: 3.862 ± 0.476
1.103SerTrp: 1.103 ± 0.224
1.165SerTyr: 1.165 ± 0.218
0.0SerXaa: 0.0 ± 0.0
Thr
5.824ThrAla: 5.824 ± 0.603
0.368ThrCys: 0.368 ± 0.136
3.372ThrAsp: 3.372 ± 0.514
3.249ThrGlu: 3.249 ± 0.398
2.268ThrPhe: 2.268 ± 0.387
6.069ThrGly: 6.069 ± 1.04
0.858ThrHis: 0.858 ± 0.256
2.82ThrIle: 2.82 ± 0.399
2.943ThrLys: 2.943 ± 0.443
5.272ThrLeu: 5.272 ± 0.609
1.226ThrMet: 1.226 ± 0.27
1.778ThrAsn: 1.778 ± 0.318
4.966ThrPro: 4.966 ± 0.64
2.759ThrGln: 2.759 ± 0.392
2.697ThrArg: 2.697 ± 0.424
2.881ThrSer: 2.881 ± 0.416
3.556ThrThr: 3.556 ± 0.559
4.966ThrVal: 4.966 ± 0.618
1.226ThrTrp: 1.226 ± 0.241
1.717ThrTyr: 1.717 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
6.253ValAla: 6.253 ± 0.743
0.552ValCys: 0.552 ± 0.216
5.517ValAsp: 5.517 ± 0.519
4.23ValGlu: 4.23 ± 0.494
2.146ValPhe: 2.146 ± 0.483
4.659ValGly: 4.659 ± 0.528
1.165ValHis: 1.165 ± 0.242
3.678ValIle: 3.678 ± 0.442
3.494ValLys: 3.494 ± 0.331
5.64ValLeu: 5.64 ± 0.621
1.717ValMet: 1.717 ± 0.352
2.881ValAsn: 2.881 ± 0.44
3.617ValPro: 3.617 ± 0.608
2.268ValGln: 2.268 ± 0.424
4.475ValArg: 4.475 ± 0.61
4.353ValSer: 4.353 ± 0.61
5.272ValThr: 5.272 ± 0.638
5.211ValVal: 5.211 ± 0.505
1.287ValTrp: 1.287 ± 0.306
2.207ValTyr: 2.207 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
1.778TrpAla: 1.778 ± 0.297
0.245TrpCys: 0.245 ± 0.163
1.226TrpAsp: 1.226 ± 0.241
1.287TrpGlu: 1.287 ± 0.259
0.429TrpPhe: 0.429 ± 0.178
1.165TrpGly: 1.165 ± 0.222
0.552TrpHis: 0.552 ± 0.203
1.042TrpIle: 1.042 ± 0.286
0.368TrpLys: 0.368 ± 0.148
1.471TrpLeu: 1.471 ± 0.334
0.49TrpMet: 0.49 ± 0.173
0.981TrpAsn: 0.981 ± 0.226
0.858TrpPro: 0.858 ± 0.246
0.858TrpGln: 0.858 ± 0.222
1.226TrpArg: 1.226 ± 0.23
1.042TrpSer: 1.042 ± 0.24
1.287TrpThr: 1.287 ± 0.237
1.41TrpVal: 1.41 ± 0.288
0.49TrpTrp: 0.49 ± 0.178
0.736TrpTyr: 0.736 ± 0.259
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.697TyrAla: 2.697 ± 0.322
0.368TyrCys: 0.368 ± 0.156
2.084TyrAsp: 2.084 ± 0.318
2.268TyrGlu: 2.268 ± 0.318
0.797TyrPhe: 0.797 ± 0.215
2.084TyrGly: 2.084 ± 0.353
0.429TyrHis: 0.429 ± 0.158
1.533TyrIle: 1.533 ± 0.267
0.736TyrLys: 0.736 ± 0.269
2.513TyrLeu: 2.513 ± 0.416
0.736TyrMet: 0.736 ± 0.197
0.92TyrAsn: 0.92 ± 0.233
1.349TyrPro: 1.349 ± 0.248
0.92TyrGln: 0.92 ± 0.226
2.513TyrArg: 2.513 ± 0.426
1.594TyrSer: 1.594 ± 0.293
1.655TyrThr: 1.655 ± 0.301
2.391TyrVal: 2.391 ± 0.479
0.552TyrTrp: 0.552 ± 0.202
0.674TyrTyr: 0.674 ± 0.223
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (16313 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski