Streptococcus phage IPP16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S8T5|A0A1S5S8T5_9CAUD Transcriptional repressor OS=Streptococcus phage IPP16 OX=1916156 GN=IPP16_00004 PE=4 SV=1
MM1 pKa = 6.9NTLNEE6 pKa = 3.81KK7 pKa = 10.4AINIFKK13 pKa = 10.8AVVAEE18 pKa = 4.07TLLQNTYY25 pKa = 7.99EE26 pKa = 4.02EE27 pKa = 4.41RR28 pKa = 11.84FLYY31 pKa = 10.0GQLEE35 pKa = 4.7SFWNNCRR42 pKa = 11.84QFAFGWTEE50 pKa = 3.47LAEE53 pKa = 4.49EE54 pKa = 4.51IEE56 pKa = 4.21RR57 pKa = 11.84QEE59 pKa = 5.23RR60 pKa = 11.84YY61 pKa = 10.28LLDD64 pKa = 4.16AGFTQDD70 pKa = 4.24EE71 pKa = 4.19IDD73 pKa = 5.57DD74 pKa = 3.87IRR76 pKa = 11.84FDD78 pKa = 3.45AAFAGMQDD86 pKa = 3.38KK87 pKa = 10.88MNVAA91 pKa = 4.28

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S8T7|A0A1S5S8T7_9CAUD Uncharacterized protein OS=Streptococcus phage IPP16 OX=1916156 GN=IPP16_00007 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.08IGMRR6 pKa = 11.84TPSLKK11 pKa = 10.35KK12 pKa = 9.79SLKK15 pKa = 10.57ARR17 pKa = 11.84TTSKK21 pKa = 9.92WKK23 pKa = 10.01RR24 pKa = 11.84QAKK27 pKa = 9.31RR28 pKa = 11.84AIIPGYY34 pKa = 9.01GKK36 pKa = 10.69KK37 pKa = 10.71GMGWIKK43 pKa = 10.52NPKK46 pKa = 8.06KK47 pKa = 10.32ALYY50 pKa = 9.9NKK52 pKa = 9.23VYY54 pKa = 10.62HH55 pKa = 6.0KK56 pKa = 8.26TTFGLSDD63 pKa = 3.87LLKK66 pKa = 10.22PSKK69 pKa = 10.0KK70 pKa = 9.05RR71 pKa = 11.84KK72 pKa = 9.52KK73 pKa = 10.41RR74 pKa = 11.84ITKK77 pKa = 9.12KK78 pKa = 10.22QSSILATRR86 pKa = 11.84SKK88 pKa = 10.98KK89 pKa = 9.42EE90 pKa = 4.06YY91 pKa = 9.87KK92 pKa = 10.14PKK94 pKa = 10.25DD95 pKa = 3.53YY96 pKa = 11.17KK97 pKa = 10.85EE98 pKa = 4.08AGIVLMVIGVVLLFLLPPIGLFLFMSGLISLIIAHH133 pKa = 6.8FSMKK137 pKa = 10.71YY138 pKa = 9.6EE139 pKa = 3.92MKK141 pKa = 10.34KK142 pKa = 8.05QQRR145 pKa = 11.84QNDD148 pKa = 3.25II149 pKa = 3.56

Molecular weight: 17.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10523

43

873

191.3

21.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.987 ± 0.378

0.618 ± 0.095

6.415 ± 0.276

7.964 ± 0.342

4.105 ± 0.199

5.844 ± 0.378

1.625 ± 0.14

7.507 ± 0.233

8.914 ± 0.387

9.237 ± 0.361

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.155

5.854 ± 0.225

2.623 ± 0.166

3.611 ± 0.166

4.181 ± 0.365

6.224 ± 0.272

5.892 ± 0.283

5.825 ± 0.233

1.245 ± 0.175

3.963 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski