Micromonospora sp. MH33

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8AQA1|A0A2P8AQA1_9ACTN NTP_transf_2 domain-containing protein OS=Micromonospora sp. MH33 OX=1945509 GN=B0E53_05434 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 3.7SHH4 pKa = 5.86QTLHH8 pKa = 7.34DD9 pKa = 3.84FVLNLLTDD17 pKa = 4.23PDD19 pKa = 3.68ARR21 pKa = 11.84SAFDD25 pKa = 4.84LDD27 pKa = 4.23PEE29 pKa = 4.73GALQAAGLTDD39 pKa = 3.29VTAADD44 pKa = 3.9VQDD47 pKa = 3.98VVPLVVDD54 pKa = 3.87YY55 pKa = 11.4APGAAGLAPVAPVAGQLGLDD75 pKa = 4.35PLTSDD80 pKa = 3.06ATDD83 pKa = 3.21VVGQLQAVPHH93 pKa = 5.8QISVNSSHH101 pKa = 7.0SGVDD105 pKa = 3.21MSAGALGAIAVDD117 pKa = 3.7PAGLYY122 pKa = 10.49AGAALVPGIGLGIGPGGLGTDD143 pKa = 3.46LTGVHH148 pKa = 6.93DD149 pKa = 4.3VANTLDD155 pKa = 4.05ADD157 pKa = 3.99VVGAVDD163 pKa = 3.55NAADD167 pKa = 3.87PVVGDD172 pKa = 4.0LAGTAGDD179 pKa = 4.27PLGTTAGVDD188 pKa = 3.69LLHH191 pKa = 7.45DD192 pKa = 4.44GGVLGGDD199 pKa = 3.69LGVLSGTQAQVDD211 pKa = 4.36GVVGSLGVHH220 pKa = 4.33QTLGGFGLGDD230 pKa = 3.52GSGVVPPVHH239 pKa = 6.99APATVDD245 pKa = 3.59GVTHH249 pKa = 5.83QVDD252 pKa = 3.56STLAGVTGTVSGVTGTVGGLTGGVTDD278 pKa = 5.38GVTGDD283 pKa = 4.12AGVHH287 pKa = 5.3GAGVHH292 pKa = 5.99GDD294 pKa = 3.5VHH296 pKa = 6.91GSTDD300 pKa = 3.23GGLLGGLLL308 pKa = 3.81

Molecular weight: 29.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8ALI0|A0A2P8ALI0_9ACTN Uncharacterized protein OS=Micromonospora sp. MH33 OX=1945509 GN=B0E53_06804 PE=4 SV=1
MM1 pKa = 7.33GGVRR5 pKa = 11.84TGRR8 pKa = 11.84HH9 pKa = 5.02RR10 pKa = 11.84PAHH13 pKa = 6.83RR14 pKa = 11.84PRR16 pKa = 11.84QQHH19 pKa = 4.82RR20 pKa = 11.84RR21 pKa = 11.84LRR23 pKa = 11.84RR24 pKa = 11.84PDD26 pKa = 3.35LPRR29 pKa = 11.84LRR31 pKa = 11.84GQGARR36 pKa = 11.84HH37 pKa = 5.84LRR39 pKa = 11.84RR40 pKa = 11.84ARR42 pKa = 11.84RR43 pKa = 11.84VPGQRR48 pKa = 11.84QRR50 pKa = 11.84RR51 pKa = 11.84QRR53 pKa = 11.84RR54 pKa = 11.84LRR56 pKa = 11.84PHH58 pKa = 7.38LLPVRR63 pKa = 11.84PRR65 pKa = 11.84RR66 pKa = 11.84PGRR69 pKa = 11.84HH70 pKa = 4.38RR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 5.85RR74 pKa = 11.84VLLVAGGPAPGRR86 pKa = 11.84PGVAPGGVRR95 pKa = 11.84ARR97 pKa = 11.84PRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84QRR104 pKa = 11.84HH105 pKa = 3.56VHH107 pKa = 5.63PRR109 pKa = 11.84HH110 pKa = 5.67ARR112 pKa = 11.84RR113 pKa = 11.84VRR115 pKa = 11.84PPARR119 pKa = 11.84PVTRR123 pKa = 11.84RR124 pKa = 11.84PVQGVRR130 pKa = 11.84RR131 pKa = 11.84GRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84HH136 pKa = 5.01RR137 pKa = 11.84LRR139 pKa = 11.84RR140 pKa = 11.84GRR142 pKa = 11.84RR143 pKa = 11.84HH144 pKa = 5.77AAAGTPLRR152 pKa = 11.84RR153 pKa = 11.84PPQRR157 pKa = 11.84PPDD160 pKa = 3.62PRR162 pKa = 11.84RR163 pKa = 11.84AARR166 pKa = 11.84QRR168 pKa = 11.84GQPGRR173 pKa = 11.84RR174 pKa = 11.84QQRR177 pKa = 11.84PHH179 pKa = 6.81RR180 pKa = 11.84PQRR183 pKa = 11.84PRR185 pKa = 11.84PAAGHH190 pKa = 6.14PPGPRR195 pKa = 11.84ARR197 pKa = 11.84AAVPVRR203 pKa = 11.84RR204 pKa = 11.84GRR206 pKa = 11.84GRR208 pKa = 11.84GPRR211 pKa = 11.84HH212 pKa = 4.88RR213 pKa = 11.84HH214 pKa = 4.57RR215 pKa = 11.84PRR217 pKa = 11.84RR218 pKa = 11.84PHH220 pKa = 6.58RR221 pKa = 11.84GAGAAGHH228 pKa = 6.69LRR230 pKa = 11.84AGPPRR235 pKa = 11.84RR236 pKa = 11.84PAAAARR242 pKa = 11.84LDD244 pKa = 4.03QVEE247 pKa = 4.16HH248 pKa = 6.86RR249 pKa = 11.84PRR251 pKa = 11.84PGRR254 pKa = 11.84RR255 pKa = 11.84RR256 pKa = 11.84RR257 pKa = 11.84RR258 pKa = 11.84RR259 pKa = 11.84GDD261 pKa = 3.01QDD263 pKa = 3.59GPRR266 pKa = 11.84HH267 pKa = 5.85AARR270 pKa = 11.84PAPGHH275 pKa = 5.49PARR278 pKa = 11.84RR279 pKa = 11.84PAVPAHH285 pKa = 6.11RR286 pKa = 11.84LDD288 pKa = 3.49RR289 pKa = 11.84RR290 pKa = 11.84RR291 pKa = 11.84GRR293 pKa = 11.84PARR296 pKa = 11.84RR297 pKa = 11.84RR298 pKa = 11.84PALARR303 pKa = 11.84RR304 pKa = 11.84GPAPPRR310 pKa = 11.84RR311 pKa = 11.84RR312 pKa = 11.84LLVRR316 pKa = 11.84HH317 pKa = 5.56QRR319 pKa = 11.84HH320 pKa = 4.0QRR322 pKa = 11.84ARR324 pKa = 11.84HH325 pKa = 5.64PGAGPRR331 pKa = 11.84AGRR334 pKa = 11.84RR335 pKa = 11.84AGRR338 pKa = 11.84AGPGPRR344 pKa = 3.94

Molecular weight: 39.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7025

0

7025

2123840

29

3432

302.3

32.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.954 ± 0.049

0.785 ± 0.009

5.983 ± 0.022

5.016 ± 0.03

2.63 ± 0.018

9.474 ± 0.035

2.243 ± 0.019

3.054 ± 0.021

1.714 ± 0.025

10.416 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.012

1.78 ± 0.019

6.485 ± 0.031

2.749 ± 0.023

8.708 ± 0.05

4.705 ± 0.027

6.042 ± 0.029

8.989 ± 0.033

1.614 ± 0.014

2.047 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski