Zymobacter palmae
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2668 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A348HI68|A0A348HI68_9GAMM Lysozyme OS=Zymobacter palmae OX=33074 GN=ZBT109_2590 PE=3 SV=1
MM1 pKa = 6.24 TTVVTSQQGDD11 pKa = 3.87 TVDD14 pKa = 5.45 LICHH18 pKa = 6.9 RR19 pKa = 11.84 YY20 pKa = 10.08 GGTSDD25 pKa = 5.4 DD26 pKa = 4.28 VIAVYY31 pKa = 10.73 ALNSGLCILGPVLPLGTRR49 pKa = 11.84 VTLPDD54 pKa = 4.37 FSTTASEE61 pKa = 4.21 QTNDD65 pKa = 3.7 TMLQLWDD72 pKa = 3.42
Molecular weight: 7.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.834
IPC_protein 3.732
Toseland 3.516
ProMoST 3.834
Dawson 3.77
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.795
Sillero 3.872
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A348HIF4|A0A348HIF4_9GAMM Uncharacterized conserved protein OS=Zymobacter palmae OX=33074 GN=ZBT109_2681 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATMRR26 pKa = 11.84 GRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2668
0
2668
844825
30
3735
316.7
34.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.59 ± 0.059
1.189 ± 0.021
5.906 ± 0.041
5.496 ± 0.048
3.451 ± 0.039
7.38 ± 0.05
2.849 ± 0.03
5.173 ± 0.046
3.102 ± 0.042
10.575 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.704 ± 0.03
2.968 ± 0.042
4.648 ± 0.044
4.606 ± 0.039
6.822 ± 0.05
5.983 ± 0.055
5.614 ± 0.044
7.009 ± 0.041
1.377 ± 0.021
2.559 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here