Pararobbsia silviterrae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Pararobbsia

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494Y1J8|A0A494Y1J8_9BURK Uncharacterized protein OS=Pararobbsia silviterrae OX=1792498 GN=D7S86_09420 PE=4 SV=1
MM1 pKa = 7.01NHH3 pKa = 6.57HH4 pKa = 7.06ASGLVDD10 pKa = 4.86RR11 pKa = 11.84DD12 pKa = 3.61PQPFAFAACSSFASKK27 pKa = 10.57RR28 pKa = 11.84FACGSPLAASLAVCAGVSVASASAARR54 pKa = 11.84AALLIDD60 pKa = 3.92TFVEE64 pKa = 4.14AAGALADD71 pKa = 5.68ADD73 pKa = 5.4DD74 pKa = 5.0EE75 pKa = 5.86DD76 pKa = 6.95DD77 pKa = 5.89DD78 pKa = 5.52VLDD81 pKa = 4.29CFGAFALALDD91 pKa = 4.72CVCDD95 pKa = 3.81ADD97 pKa = 3.8AAFAARR103 pKa = 11.84PAFADD108 pKa = 3.3

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494XGH4|A0A494XGH4_9BURK Enoyl-[acyl-carrier-protein] reductase [NADH] OS=Pararobbsia silviterrae OX=1792498 GN=fabV PE=3 SV=1
RR1 pKa = 7.46GSGFGVRR8 pKa = 11.84GSGFGVRR15 pKa = 11.84GSGFGVRR22 pKa = 11.84GSGFGVRR29 pKa = 11.84GSGLGARR36 pKa = 11.84GSGLGARR43 pKa = 11.84GSGLGARR50 pKa = 11.84GSGFRR55 pKa = 11.84VQGSGLGARR64 pKa = 11.84GRR66 pKa = 11.84GLGARR71 pKa = 11.84ALRR74 pKa = 4.07

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5622

0

5622

1887409

31

4281

335.7

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.977 ± 0.045

0.876 ± 0.011

5.859 ± 0.028

4.886 ± 0.034

3.588 ± 0.02

8.154 ± 0.036

2.43 ± 0.016

4.958 ± 0.023

2.767 ± 0.03

9.952 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.014

2.712 ± 0.024

5.021 ± 0.024

3.347 ± 0.023

7.15 ± 0.045

5.868 ± 0.031

5.694 ± 0.04

7.774 ± 0.027

1.338 ± 0.013

2.397 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski