Planctomycetes bacterium Pla163

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3741 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518D480|A0A518D480_9BACT Phosphatidylglycerophosphatase A OS=Planctomycetes bacterium Pla163 OX=2528008 GN=pgpA PE=4 SV=1
MM1 pKa = 7.43NPLLSGALFTALSPLALAGGTEE23 pKa = 4.61ASSDD27 pKa = 3.12WLGLDD32 pKa = 3.47EE33 pKa = 5.07EE34 pKa = 4.98LNAVASNVSLQGGGVEE50 pKa = 4.71LGALFRR56 pKa = 11.84GAYY59 pKa = 9.63VMADD63 pKa = 2.95GTDD66 pKa = 3.84FNGFADD72 pKa = 4.35GNGDD76 pKa = 4.49GNPDD80 pKa = 3.63DD81 pKa = 3.9QLGMVVDD88 pKa = 5.22DD89 pKa = 5.03AEE91 pKa = 4.51LWSQGTVGDD100 pKa = 4.12YY101 pKa = 9.84FWRR104 pKa = 11.84ISVDD108 pKa = 3.61FGTQFSPYY116 pKa = 9.83FIGGLIGTGAGTSAFANTARR136 pKa = 11.84TDD138 pKa = 3.77TLEE141 pKa = 4.35DD142 pKa = 4.05AYY144 pKa = 10.18IDD146 pKa = 3.58WRR148 pKa = 11.84FSDD151 pKa = 3.81QFSLRR156 pKa = 11.84LGHH159 pKa = 7.04FILPTTFSAASNPDD173 pKa = 3.35SLLMLGRR180 pKa = 11.84TTSGEE185 pKa = 3.96YY186 pKa = 9.42FHH188 pKa = 7.64NYY190 pKa = 9.82DD191 pKa = 4.25LGAMLSGDD199 pKa = 3.46YY200 pKa = 10.78EE201 pKa = 4.09QFQWHH206 pKa = 6.39LAATNGFDD214 pKa = 4.03GTGDD218 pKa = 3.53EE219 pKa = 3.97QRR221 pKa = 11.84LFGRR225 pKa = 11.84AIYY228 pKa = 10.56NLGGGQNATEE238 pKa = 4.34GALGADD244 pKa = 4.0EE245 pKa = 4.95NLNAAIGATFWDD257 pKa = 5.2DD258 pKa = 3.56GTVDD262 pKa = 4.06DD263 pKa = 4.84SDD265 pKa = 4.21VVAFDD270 pKa = 3.35VSATVGQISFSGEE283 pKa = 3.51FVTYY287 pKa = 10.72GDD289 pKa = 3.61AHH291 pKa = 5.9ATAAAGTFAGATNLGYY307 pKa = 8.44TAGSGPSVWGLTVSYY322 pKa = 10.45LLNEE326 pKa = 4.19EE327 pKa = 4.1WEE329 pKa = 4.15IAARR333 pKa = 11.84MEE335 pKa = 5.58DD336 pKa = 3.78MDD338 pKa = 5.49DD339 pKa = 3.7LANTEE344 pKa = 3.97IMSFGLNYY352 pKa = 10.13YY353 pKa = 9.97QLGHH357 pKa = 5.44NAKK360 pKa = 8.22WQLMLMSVSSDD371 pKa = 3.34VAVNEE376 pKa = 4.19GSVIALGVTVGSNGG390 pKa = 3.16

Molecular weight: 41.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518D0A3|A0A518D0A3_9BACT Uncharacterized protein OS=Planctomycetes bacterium Pla163 OX=2528008 GN=Pla163_19930 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.6SKK4 pKa = 10.95GSVKK8 pKa = 10.19KK9 pKa = 10.61RR10 pKa = 11.84FRR12 pKa = 11.84TTKK15 pKa = 10.0NGKK18 pKa = 9.75LIANKK23 pKa = 7.23TARR26 pKa = 11.84GHH28 pKa = 4.21MHH30 pKa = 6.63APKK33 pKa = 10.15NGKK36 pKa = 8.69ARR38 pKa = 11.84RR39 pKa = 11.84GKK41 pKa = 10.22RR42 pKa = 11.84KK43 pKa = 8.98PLVISGTWAVLMKK56 pKa = 10.94RR57 pKa = 11.84MMM59 pKa = 4.28

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3741

0

3741

1465947

32

2399

391.9

42.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.806 ± 0.055

0.883 ± 0.012

6.653 ± 0.036

6.665 ± 0.039

3.437 ± 0.024

9.256 ± 0.043

2.028 ± 0.019

3.368 ± 0.026

1.831 ± 0.027

10.738 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.593 ± 0.016

1.921 ± 0.024

5.502 ± 0.026

2.477 ± 0.022

8.393 ± 0.05

5.552 ± 0.027

5.326 ± 0.024

8.292 ± 0.038

1.489 ± 0.015

1.791 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski