Fonsecaea erecta
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12089 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A178ZQF3|A0A178ZQF3_9EURO ULP_PROTEASE domain-containing protein OS=Fonsecaea erecta OX=1367422 GN=AYL99_03894 PE=3 SV=1
MM1 pKa = 7.83 IPILLCSLLLALVQFGVSQTVSSSDD26 pKa = 3.13 GYY28 pKa = 9.14 TGYY31 pKa = 10.9 SLSVTGNDD39 pKa = 3.02 TDD41 pKa = 3.83 SAVYY45 pKa = 8.31 EE46 pKa = 4.29 TEE48 pKa = 4.03 EE49 pKa = 4.35 TDD51 pKa = 3.44 TSDD54 pKa = 4.6 GAVTDD59 pKa = 4.24 ASTPPDD65 pKa = 3.45 VYY67 pKa = 11.34 LNASVSVGEE76 pKa = 4.38 IDD78 pKa = 3.22 ITVKK82 pKa = 10.74 NLSAKK87 pKa = 10.22 INIDD91 pKa = 3.52 AQVLSLLQFNAGVDD105 pKa = 3.6 LSINAVVLNIQNVTAKK121 pKa = 10.59 VEE123 pKa = 4.04 LEE125 pKa = 3.71 ARR127 pKa = 11.84 LEE129 pKa = 3.92 NLVLMINDD137 pKa = 3.94 TLNSIDD143 pKa = 4.87 LNPIIATLGSDD154 pKa = 3.21 VGSLVGTALGGSSTSSSSASSSTSGLSARR183 pKa = 11.84 SVLLDD188 pKa = 3.3 EE189 pKa = 5.7 GILYY193 pKa = 10.36 SVNDD197 pKa = 3.67 YY198 pKa = 11.33 SGNTHH203 pKa = 6.2 TNRR206 pKa = 11.84 ILDD209 pKa = 3.53 QAGNIVDD216 pKa = 3.9 QKK218 pKa = 10.56 LHH220 pKa = 6.63 NDD222 pKa = 3.03 GSVYY226 pKa = 10.36 SSQVVGSYY234 pKa = 6.62 WHH236 pKa = 7.24 DD237 pKa = 3.32 MAFTGHH243 pKa = 5.46 EE244 pKa = 3.91 RR245 pKa = 11.84 AVLFNGEE252 pKa = 3.97 NAKK255 pKa = 10.11 EE256 pKa = 3.99 LEE258 pKa = 4.23 YY259 pKa = 10.8 SYY261 pKa = 11.78 SPFNGFSSIAAIYY274 pKa = 10.28 QDD276 pKa = 3.08 AQGNVLGTRR285 pKa = 11.84 VLSEE289 pKa = 3.94 IEE291 pKa = 4.21 GGGSSTIADD300 pKa = 3.81 DD301 pKa = 3.65
Molecular weight: 31.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.859
IPC_protein 3.872
Toseland 3.656
ProMoST 3.986
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.986
Patrickios 1.163
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A178Z9G9|A0A178Z9G9_9EURO NodB homology domain-containing protein OS=Fonsecaea erecta OX=1367422 GN=AYL99_09608 PE=4 SV=1
MM1 pKa = 7.15 HH2 pKa = 7.78 RR3 pKa = 11.84 EE4 pKa = 3.78 THH6 pKa = 5.49 IHH8 pKa = 6.86 LKK10 pKa = 7.56 TLKK13 pKa = 9.86 PLLRR17 pKa = 11.84 AYY19 pKa = 10.41 ALGYY23 pKa = 8.8 LTVTAPRR30 pKa = 11.84 LFGFLRR36 pKa = 11.84 TLRR39 pKa = 11.84 RR40 pKa = 11.84 SHH42 pKa = 6.12 LASRR46 pKa = 11.84 EE47 pKa = 3.93 VFSILRR53 pKa = 11.84 RR54 pKa = 11.84 ILITSTQLNRR64 pKa = 11.84 FPTAAAVIVGGATALPRR81 pKa = 11.84 LLLATLEE88 pKa = 4.53 GVLSLLRR95 pKa = 11.84 KK96 pKa = 9.88 GPPTRR101 pKa = 11.84 LSRR104 pKa = 11.84 SIVGLIHH111 pKa = 6.34 FLCSFWSAWLAFALLNRR128 pKa = 11.84 DD129 pKa = 3.62 EE130 pKa = 4.21 TWVRR134 pKa = 11.84 KK135 pKa = 9.2 RR136 pKa = 11.84 AISRR140 pKa = 11.84 ASPNTDD146 pKa = 2.51 ASALGASTLHH156 pKa = 5.83 QPSPPSYY163 pKa = 8.57 HH164 pKa = 6.21 PHH166 pKa = 5.92 YY167 pKa = 10.34 AGKK170 pKa = 8.75 TIDD173 pKa = 3.48 FTAFAFCRR181 pKa = 11.84 AVDD184 pKa = 3.71 VMVITLWTRR193 pKa = 11.84 TRR195 pKa = 11.84 TRR197 pKa = 11.84 SWHH200 pKa = 5.7 PEE202 pKa = 3.32 QRR204 pKa = 11.84 NPRR207 pKa = 11.84 LSNLVRR213 pKa = 11.84 KK214 pKa = 9.02 LADD217 pKa = 3.54 PSVFAGSAAIIMWSWFYY234 pKa = 11.36 SPEE237 pKa = 3.93 RR238 pKa = 11.84 LPRR241 pKa = 11.84 SYY243 pKa = 11.04 NQWISKK249 pKa = 10.07 AADD252 pKa = 3.12 IDD254 pKa = 3.64 PRR256 pKa = 11.84 LIQALRR262 pKa = 11.84 LARR265 pKa = 11.84 QGNFVYY271 pKa = 11.02 GEE273 pKa = 4.2 DD274 pKa = 3.47 TGQAPLLAGLCRR286 pKa = 11.84 EE287 pKa = 4.19 LDD289 pKa = 3.8 LPEE292 pKa = 4.69 EE293 pKa = 4.33 FADD296 pKa = 4.0 PSKK299 pKa = 9.97 TIPIPCEE306 pKa = 3.86 LYY308 pKa = 9.99 HH309 pKa = 6.54 CGSGKK314 pKa = 9.94 SCEE317 pKa = 3.69 IHH319 pKa = 6.09 ALSRR323 pKa = 11.84 FWRR326 pKa = 11.84 SWKK329 pKa = 9.96 FAMEE333 pKa = 4.26 IYY335 pKa = 10.71 LPLQLLTILRR345 pKa = 11.84 SPRR348 pKa = 11.84 PKK350 pKa = 10.44 SVLKK354 pKa = 10.54 VLQAASRR361 pKa = 11.84 SSSFLSGFVASFYY374 pKa = 10.97 YY375 pKa = 10.16 AVCLARR381 pKa = 11.84 TRR383 pKa = 11.84 LGPKK387 pKa = 9.43 IFSQRR392 pKa = 11.84 TVTPQMWDD400 pKa = 2.58 SGLCVLTGCLACGWSVLLEE419 pKa = 4.3 KK420 pKa = 10.34 PSRR423 pKa = 11.84 RR424 pKa = 11.84 QEE426 pKa = 3.51 IAFFVAPRR434 pKa = 11.84 ALATILPRR442 pKa = 11.84 VYY444 pKa = 10.06 DD445 pKa = 3.07 QQYY448 pKa = 9.05 RR449 pKa = 11.84 HH450 pKa = 6.41 RR451 pKa = 11.84 EE452 pKa = 3.65 QAIFATSIAVVLTALKK468 pKa = 10.53 SGNEE472 pKa = 3.73 RR473 pKa = 11.84 NIRR476 pKa = 11.84 GVLGRR481 pKa = 11.84 ILGGILKK488 pKa = 10.22 DD489 pKa = 3.5
Molecular weight: 54.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.706
IPC_protein 10.613
Toseland 10.599
ProMoST 10.379
Dawson 10.73
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.862
Grimsley 10.789
Solomon 10.847
Lehninger 10.804
Nozaki 10.613
DTASelect 10.482
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.672
Patrickios 10.511
IPC_peptide 10.847
IPC2_peptide 9.721
IPC2.peptide.svr19 8.46
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12089
0
12089
5948075
39
6060
492.0
54.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.745 ± 0.019
1.224 ± 0.008
5.71 ± 0.015
6.091 ± 0.023
3.727 ± 0.014
6.879 ± 0.02
2.47 ± 0.011
4.823 ± 0.013
4.653 ± 0.019
8.972 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.17 ± 0.007
3.528 ± 0.01
6.107 ± 0.022
4.121 ± 0.015
6.195 ± 0.018
8.065 ± 0.023
6.039 ± 0.017
6.24 ± 0.014
1.485 ± 0.009
2.756 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here