Pacific flying fox faeces associated gemycircularvirus-7
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTN4|A0A140CTN4_9VIRU RepA OS=Pacific flying fox faeces associated gemycircularvirus-7 OX=1795999 PE=4 SV=1
MM1 pKa = 6.83 PAKK4 pKa = 10.46 YY5 pKa = 10.31 KK6 pKa = 10.68 LDD8 pKa = 3.58 NEE10 pKa = 4.36 QFFMVTFVRR19 pKa = 11.84 TPDD22 pKa = 3.35 DD23 pKa = 3.64 WPVCDD28 pKa = 6.22 LITILDD34 pKa = 4.05 RR35 pKa = 11.84 LGCDD39 pKa = 3.17 YY40 pKa = 11.01 RR41 pKa = 11.84 VGRR44 pKa = 11.84 EE45 pKa = 3.6 LHH47 pKa = 6.89 ADD49 pKa = 4.2 GKK51 pKa = 8.6 PHH53 pKa = 5.13 YY54 pKa = 10.11 HH55 pKa = 6.52 AMCVFDD61 pKa = 5.42 EE62 pKa = 5.39 PYY64 pKa = 10.89 SDD66 pKa = 3.19 TDD68 pKa = 3.13 ARR70 pKa = 11.84 KK71 pKa = 7.12 TFQVAGRR78 pKa = 11.84 SPNIRR83 pKa = 11.84 VRR85 pKa = 11.84 RR86 pKa = 11.84 SRR88 pKa = 11.84 PDD90 pKa = 3.07 RR91 pKa = 11.84 GWNYY95 pKa = 9.65 VGKK98 pKa = 9.54 HH99 pKa = 5.51 AGTKK103 pKa = 9.22 EE104 pKa = 3.67 GHH106 pKa = 6.51 EE107 pKa = 4.16 IVAEE111 pKa = 4.1 RR112 pKa = 11.84 GEE114 pKa = 4.42 CPMGDD119 pKa = 3.56 EE120 pKa = 4.82 EE121 pKa = 5.72 DD122 pKa = 4.24 RR123 pKa = 11.84 PSNDD127 pKa = 2.35 VWHH130 pKa = 6.84 EE131 pKa = 3.99 IILARR136 pKa = 11.84 TRR138 pKa = 11.84 EE139 pKa = 4.03 EE140 pKa = 4.11 FFEE143 pKa = 4.59 KK144 pKa = 10.13 CATMAPRR151 pKa = 11.84 QLACCFGQLSSYY163 pKa = 11.23 ADD165 pKa = 3.14 WKK167 pKa = 8.81 YY168 pKa = 11.32 AKK170 pKa = 9.45 PKK172 pKa = 9.86 EE173 pKa = 4.47 VYY175 pKa = 9.02 EE176 pKa = 4.24 PPVGEE181 pKa = 3.86 FLEE184 pKa = 4.6 IPEE187 pKa = 4.84 EE188 pKa = 3.91 LTSWVEE194 pKa = 4.26 RR195 pKa = 11.84 NLTNPNGRR203 pKa = 11.84 YY204 pKa = 9.31 VCII207 pKa = 4.32
Molecular weight: 24.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.238
IPC2_protein 5.334
IPC_protein 5.283
Toseland 5.359
ProMoST 5.486
Dawson 5.359
Bjellqvist 5.448
Wikipedia 5.258
Rodwell 5.296
Grimsley 5.334
Solomon 5.359
Lehninger 5.321
Nozaki 5.525
DTASelect 5.664
Thurlkill 5.448
EMBOSS 5.397
Sillero 5.601
Patrickios 3.897
IPC_peptide 5.372
IPC2_peptide 5.601
IPC2.peptide.svr19 5.591
Protein with the highest isoelectric point:
>tr|A0A140CTN3|A0A140CTN3_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-7 OX=1795999 PE=4 SV=1
MM1 pKa = 7.91 PYY3 pKa = 10.48 GRR5 pKa = 11.84 YY6 pKa = 9.09 RR7 pKa = 11.84 RR8 pKa = 11.84 SSYY11 pKa = 10.18 RR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 10.12 GRR16 pKa = 11.84 SRR18 pKa = 11.84 YY19 pKa = 9.71 APRR22 pKa = 11.84 SYY24 pKa = 10.88 VRR26 pKa = 11.84 RR27 pKa = 11.84 PYY29 pKa = 10.54 YY30 pKa = 9.73 GVRR33 pKa = 11.84 RR34 pKa = 11.84 PFYY37 pKa = 9.15 SRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 YY42 pKa = 9.87 RR43 pKa = 11.84 PVSRR47 pKa = 11.84 SILNVSSVKK56 pKa = 10.75 KK57 pKa = 9.57 KK58 pKa = 11.17 DD59 pKa = 3.37 NMLAVTNVLGTARR72 pKa = 11.84 PSVQPFQDD80 pKa = 3.56 GAASLVGSTAGRR92 pKa = 11.84 GSPYY96 pKa = 10.45 VFIWNATARR105 pKa = 11.84 DD106 pKa = 4.0 HH107 pKa = 7.23 EE108 pKa = 5.05 DD109 pKa = 3.84 DD110 pKa = 3.52 STKK113 pKa = 10.41 YY114 pKa = 10.76 DD115 pKa = 3.37 EE116 pKa = 4.61 AARR119 pKa = 11.84 TSSAPYY125 pKa = 9.39 MVGLKK130 pKa = 10.06 EE131 pKa = 4.46 RR132 pKa = 11.84 ILVQTSDD139 pKa = 3.41 ANPWQWRR146 pKa = 11.84 RR147 pKa = 11.84 ICFTAKK153 pKa = 10.22 GLNSSFLTAGAGANEE168 pKa = 4.09 NNPFYY173 pKa = 10.72 EE174 pKa = 4.8 DD175 pKa = 3.0 STGFRR180 pKa = 11.84 RR181 pKa = 11.84 EE182 pKa = 3.73 VAEE185 pKa = 4.62 PGSAGLTNIRR195 pKa = 11.84 NIIFDD200 pKa = 3.8 GAYY203 pKa = 10.33 GIDD206 pKa = 3.48 WNDD209 pKa = 3.35 PFSAKK214 pKa = 9.49 TDD216 pKa = 3.34 NQRR219 pKa = 11.84 IKK221 pKa = 10.85 VRR223 pKa = 11.84 YY224 pKa = 9.2 DD225 pKa = 2.88 KK226 pKa = 11.24 TRR228 pKa = 11.84 MIRR231 pKa = 11.84 STNNVGVIRR240 pKa = 11.84 YY241 pKa = 9.1 YY242 pKa = 11.65 NMWHH246 pKa = 6.59 PMKK249 pKa = 9.8 KK250 pKa = 8.15 TLMYY254 pKa = 10.94 NDD256 pKa = 4.95 DD257 pKa = 3.99 EE258 pKa = 5.71 NGASMDD264 pKa = 3.67 SAYY267 pKa = 10.05 VAQMGDD273 pKa = 3.36 MGMGDD278 pKa = 4.13 YY279 pKa = 10.6 FVLDD283 pKa = 4.97 FIRR286 pKa = 11.84 CIDD289 pKa = 4.16 APSGTPGLIFQPEE302 pKa = 3.88 ATLYY306 pKa = 7.84 WHH308 pKa = 6.69 EE309 pKa = 4.18 RR310 pKa = 3.47
Molecular weight: 35.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.772
IPC2_protein 9.458
IPC_protein 9.882
Toseland 9.589
ProMoST 9.648
Dawson 9.97
Bjellqvist 9.765
Wikipedia 10.233
Rodwell 10.028
Grimsley 10.101
Solomon 9.984
Lehninger 9.926
Nozaki 9.619
DTASelect 9.75
Thurlkill 9.75
EMBOSS 10.043
Sillero 9.882
Patrickios 4.584
IPC_peptide 9.97
IPC2_peptide 8.668
IPC2.peptide.svr19 8.33
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
848
207
331
282.7
32.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.722 ± 0.628
2.241 ± 0.837
7.075 ± 0.293
6.722 ± 1.736
4.245 ± 0.176
7.075 ± 0.373
2.358 ± 0.649
4.245 ± 0.165
5.071 ± 0.786
5.307 ± 0.548
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.066 ± 0.287
4.127 ± 0.784
6.25 ± 0.447
2.123 ± 0.082
8.844 ± 1.03
5.542 ± 1.385
5.307 ± 0.178
5.66 ± 0.46
2.83 ± 0.517
5.189 ± 0.891
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here