Marispirochaeta aestuarii

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Marispirochaeta

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3763 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1RYF4|A0A1Y1RYF4_9SPIO Chemotaxis protein OS=Marispirochaeta aestuarii OX=1963862 GN=B4O97_08160 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.2KK3 pKa = 9.84IVVVLVMLSLLVLPALAQTSFGLKK27 pKa = 9.94GALSDD32 pKa = 4.41YY33 pKa = 11.04SWTGDD38 pKa = 2.78GWGDD42 pKa = 3.38VQDD45 pKa = 4.5YY46 pKa = 10.14YY47 pKa = 11.47EE48 pKa = 4.24FMYY51 pKa = 10.53GVEE54 pKa = 4.01VDD56 pKa = 3.8NEE58 pKa = 4.04FSFGFSIGGFIEE70 pKa = 4.56HH71 pKa = 6.79KK72 pKa = 10.53VSSTLAIQPEE82 pKa = 4.56LLFTLASMQYY92 pKa = 11.11GDD94 pKa = 3.58GDD96 pKa = 3.46DD97 pKa = 4.44WIRR100 pKa = 11.84EE101 pKa = 4.03TWKK104 pKa = 9.55MLEE107 pKa = 3.71IPVYY111 pKa = 10.71LKK113 pKa = 11.19GLFPLDD119 pKa = 3.2QGSFYY124 pKa = 11.03IMGGPDD130 pKa = 3.94LFYY133 pKa = 11.26LLGDD137 pKa = 3.82IEE139 pKa = 5.6IDD141 pKa = 3.53TSNDD145 pKa = 3.1TSSSDD150 pKa = 4.42DD151 pKa = 4.66DD152 pKa = 4.41YY153 pKa = 11.99DD154 pKa = 4.17NNLLFGFAVSAGYY167 pKa = 9.86EE168 pKa = 4.12FQNGAFLGLKK178 pKa = 9.96FSRR181 pKa = 11.84VLTEE185 pKa = 4.58YY186 pKa = 11.42YY187 pKa = 10.72DD188 pKa = 3.85DD189 pKa = 3.72TDD191 pKa = 4.79LFIHH195 pKa = 7.18GIGIEE200 pKa = 4.13GGMKK204 pKa = 10.29LL205 pKa = 4.29

Molecular weight: 22.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1S1U5|A0A1Y1S1U5_9SPIO Sugar ABC transporter substrate-binding protein OS=Marispirochaeta aestuarii OX=1963862 GN=B4O97_01735 PE=4 SV=1
MM1 pKa = 7.38NSCRR5 pKa = 11.84NISINLLRR13 pKa = 11.84WKK15 pKa = 9.4LCCGAALRR23 pKa = 11.84VAGINLRR30 pKa = 11.84YY31 pKa = 8.37LTAGTRR37 pKa = 11.84ILTLLSVSGCGSFKK51 pKa = 10.65QKK53 pKa = 10.17FRR55 pKa = 11.84FSRR58 pKa = 11.84RR59 pKa = 11.84VCTFHH64 pKa = 7.31GLTILIDD71 pKa = 3.62FVRR74 pKa = 11.84AARR77 pKa = 11.84TPSKK81 pKa = 10.01EE82 pKa = 3.56WKK84 pKa = 9.73LNLSAGEE91 pKa = 3.94DD92 pKa = 3.63TLNYY96 pKa = 9.83LHH98 pKa = 6.6SEE100 pKa = 3.86KK101 pKa = 10.53AIRR104 pKa = 11.84LQRR107 pKa = 11.84TIAKK111 pKa = 9.74HH112 pKa = 5.4FIEE115 pKa = 4.45RR116 pKa = 11.84QYY118 pKa = 11.32GIRR121 pKa = 11.84PEE123 pKa = 4.06PFEE126 pKa = 5.42LEE128 pKa = 4.23VLSRR132 pKa = 11.84CASVGAASGRR142 pKa = 11.84LIRR145 pKa = 11.84AAGCGSPTTLNRR157 pKa = 11.84EE158 pKa = 3.84DD159 pKa = 4.41LFRR162 pKa = 11.84CRR164 pKa = 11.84TVFPAFIRR172 pKa = 11.84LLNLDD177 pKa = 3.0MDD179 pKa = 4.05FRR181 pKa = 11.84EE182 pKa = 4.36RR183 pKa = 11.84NPVPEE188 pKa = 4.77PPLSRR193 pKa = 11.84FRR195 pKa = 11.84IKK197 pKa = 10.52LRR199 pKa = 11.84RR200 pKa = 11.84SLSLPRR206 pKa = 11.84RR207 pKa = 11.84TRR209 pKa = 11.84QPALPQLYY217 pKa = 9.13PRR219 pKa = 11.84EE220 pKa = 4.02LDD222 pKa = 3.18RR223 pKa = 11.84EE224 pKa = 3.9RR225 pKa = 11.84RR226 pKa = 11.84EE227 pKa = 3.95FEE229 pKa = 4.07RR230 pKa = 11.84TSLLARR236 pKa = 11.84YY237 pKa = 8.56CSSKK241 pKa = 10.83AILVRR246 pKa = 11.84TFTSEE251 pKa = 4.39LFRR254 pKa = 11.84LYY256 pKa = 10.56LDD258 pKa = 3.93AMQLIIEE265 pKa = 4.69NPPSKK270 pKa = 10.7AGLHH274 pKa = 5.95NIEE277 pKa = 4.58PLLNRR282 pKa = 11.84LEE284 pKa = 4.16RR285 pKa = 11.84MHH287 pKa = 6.56TKK289 pKa = 10.21SLNHH293 pKa = 5.87FRR295 pKa = 11.84EE296 pKa = 3.98EE297 pKa = 4.01NYY299 pKa = 10.93GYY301 pKa = 9.75MEE303 pKa = 4.25

Molecular weight: 35.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3763

0

3763

1298959

37

3619

345.2

38.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.054 ± 0.043

0.889 ± 0.014

5.467 ± 0.034

7.412 ± 0.045

4.473 ± 0.027

7.86 ± 0.034

1.773 ± 0.017

6.888 ± 0.042

4.537 ± 0.042

10.514 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.02

3.433 ± 0.033

4.365 ± 0.028

2.912 ± 0.021

6.497 ± 0.039

6.808 ± 0.034

4.797 ± 0.028

6.603 ± 0.03

1.008 ± 0.013

3.268 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski