Stygiolobus rod-shaped virus
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6EFC6|B6EFC6_9VIRU Uncharacterized protein OS=Stygiolobus rod-shaped virus OX=537009 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 4.78 ICEE5 pKa = 4.24 VLEE8 pKa = 5.29 KK9 pKa = 10.16 ITDD12 pKa = 3.79 DD13 pKa = 3.23 KK14 pKa = 11.7 CYY16 pKa = 10.98 DD17 pKa = 3.59 SLEE20 pKa = 4.72 AEE22 pKa = 4.78 AEE24 pKa = 3.94 NDD26 pKa = 4.01 DD27 pKa = 3.81 IFIPVYY33 pKa = 9.66 IFKK36 pKa = 10.38 IKK38 pKa = 10.41 YY39 pKa = 9.03 LYY41 pKa = 10.52 KK42 pKa = 10.56 FEE44 pKa = 5.28 IDD46 pKa = 3.31 NSSVLIHH53 pKa = 6.51 FSSTRR58 pKa = 11.84 HH59 pKa = 6.28 DD60 pKa = 3.47 IEE62 pKa = 4.66 IEE64 pKa = 4.09 YY65 pKa = 10.08 IYY67 pKa = 10.9 KK68 pKa = 10.43 DD69 pKa = 3.65 VIKK72 pKa = 10.49 IIIDD76 pKa = 3.48 GEE78 pKa = 4.15 EE79 pKa = 3.91 YY80 pKa = 10.34 RR81 pKa = 11.84 CEE83 pKa = 4.07 KK84 pKa = 10.82 EE85 pKa = 4.2 CMFDD89 pKa = 3.26 ISFSGDD95 pKa = 3.31 TIEE98 pKa = 5.78 LRR100 pKa = 11.84 LYY102 pKa = 10.97 DD103 pKa = 4.49 CVDD106 pKa = 4.04 SII108 pKa = 5.3
Molecular weight: 12.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.897
IPC2_protein 4.177
IPC_protein 4.113
Toseland 3.935
ProMoST 4.24
Dawson 4.075
Bjellqvist 4.228
Wikipedia 3.973
Rodwell 3.948
Grimsley 3.846
Solomon 4.075
Lehninger 4.024
Nozaki 4.19
DTASelect 4.368
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.228
Patrickios 1.138
IPC_peptide 4.075
IPC2_peptide 4.215
IPC2.peptide.svr19 4.113
Protein with the highest isoelectric point:
>tr|B6EFC2|B6EFC2_9VIRU Uncharacterized protein OS=Stygiolobus rod-shaped virus OX=537009 PE=4 SV=1
MM1 pKa = 7.66 AKK3 pKa = 10.15 GRR5 pKa = 11.84 TPRR8 pKa = 11.84 SFTQRR13 pKa = 11.84 YY14 pKa = 8.34 AKK16 pKa = 9.95 WNAKK20 pKa = 8.03 FTAFSNPTVASTILSNVAAIAQQNFQTNVPKK51 pKa = 10.62 FSSVNEE57 pKa = 3.91 QVSAVLTEE65 pKa = 4.44 LGVTGPNRR73 pKa = 11.84 AIYY76 pKa = 9.58 QGYY79 pKa = 8.37 ALKK82 pKa = 10.53 VARR85 pKa = 11.84 ALNRR89 pKa = 11.84 LGGGQALVNMINGLKK104 pKa = 10.01 AYY106 pKa = 9.82 YY107 pKa = 9.87 ISAFNANPSVLDD119 pKa = 3.48 AVTNIITGSPYY130 pKa = 10.69 GYY132 pKa = 10.89 VSS134 pKa = 3.13
Molecular weight: 14.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.25
IPC2_protein 10.072
IPC_protein 10.467
Toseland 10.233
ProMoST 10.028
Dawson 10.467
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.818
Grimsley 10.57
Solomon 10.511
Lehninger 10.467
Nozaki 10.204
DTASelect 10.189
Thurlkill 10.306
EMBOSS 10.643
Sillero 10.379
Patrickios 10.452
IPC_peptide 10.496
IPC2_peptide 8.99
IPC2.peptide.svr19 8.529
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
37
0
37
7990
52
1059
215.9
24.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.743 ± 0.35
0.914 ± 0.205
4.243 ± 0.389
6.608 ± 0.685
5.507 ± 0.373
4.043 ± 0.285
1.227 ± 0.207
10.325 ± 0.45
7.159 ± 0.577
10.225 ± 0.678
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.802 ± 0.163
7.397 ± 0.608
3.542 ± 0.223
4.743 ± 0.419
3.392 ± 0.351
6.834 ± 0.468
4.944 ± 0.277
5.569 ± 0.324
0.801 ± 0.108
5.982 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here