Microviridae Fen7786_21

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UEG1|A0A0G2UEG1_9VIRU Uncharacterized protein OS=Microviridae Fen7786_21 OX=1655658 PE=4 SV=1
MM1 pKa = 7.64LIDD4 pKa = 3.8TSQFLSYY11 pKa = 10.33LKK13 pKa = 10.29HH14 pKa = 7.24SFMMYY19 pKa = 9.09LQVMASAGGLSAGQVLGAIGGALPVVGGIVSSLINNADD57 pKa = 3.4TTANNQANEE66 pKa = 4.0QFQLQQQQAQDD77 pKa = 3.5TYY79 pKa = 9.93NTQMWNNQNAYY90 pKa = 9.41NAPDD94 pKa = 3.35AQMSRR99 pKa = 11.84LEE101 pKa = 4.25DD102 pKa = 3.63AGLNPNLVYY111 pKa = 10.89GSGNVTGNVAEE122 pKa = 5.03AAPSMAKK129 pKa = 9.84VEE131 pKa = 4.4YY132 pKa = 10.64VSAPQRR138 pKa = 11.84APDD141 pKa = 3.83LASDD145 pKa = 4.37LTSGLAAYY153 pKa = 10.22NNLQLGSAQSDD164 pKa = 3.23NLAAQTHH171 pKa = 6.57LANSTSQLQSTEE183 pKa = 3.77AALNAASTDD192 pKa = 3.24KK193 pKa = 10.83TVAEE197 pKa = 4.56TSTIPAITAATVANMQSQTFKK218 pKa = 11.16NDD220 pKa = 2.2VDD222 pKa = 4.31SFVSSTLLPLQEE234 pKa = 4.64GQLSADD240 pKa = 3.4ATASLAAARR249 pKa = 11.84ANLTNAQQNVIKK261 pKa = 9.53TAVDD265 pKa = 3.54VKK267 pKa = 10.54TGIAQIAALAIQNAKK282 pKa = 9.98SQTEE286 pKa = 3.81IANIKK291 pKa = 9.66AQTALVLGNKK301 pKa = 9.25DD302 pKa = 3.75LQKK305 pKa = 10.18MDD307 pKa = 2.58ITMRR311 pKa = 11.84QQGEE315 pKa = 4.27MPNNSAAGNVIGAFATWLTGHH336 pKa = 6.02NQAGGYY342 pKa = 8.93NSNVTPNAIKK352 pKa = 10.69LSDD355 pKa = 3.64GTQINPDD362 pKa = 3.17GTTTKK367 pKa = 10.53QPP369 pKa = 3.37

Molecular weight: 38.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UG51|A0A0G2UG51_9VIRU Uncharacterized protein OS=Microviridae Fen7786_21 OX=1655658 PE=4 SV=1
MM1 pKa = 7.26PTGSFPNRR9 pKa = 11.84IKK11 pKa = 9.93KK12 pKa = 7.14TCRR15 pKa = 11.84TCFRR19 pKa = 11.84LLLALLQRR27 pKa = 11.84LSSTNTFQFTVTLTISSALKK47 pKa = 8.94TKK49 pKa = 10.18PVTLLFVHH57 pKa = 6.59FNKK60 pKa = 10.38RR61 pKa = 11.84LNAAQINMKK70 pKa = 10.15KK71 pKa = 10.4ADD73 pKa = 3.38VHH75 pKa = 6.67SRR77 pKa = 11.84IVNHH81 pKa = 5.97LTYY84 pKa = 11.0SHH86 pKa = 7.45LEE88 pKa = 4.0TMEE91 pKa = 3.91LVLWKK96 pKa = 10.5RR97 pKa = 11.84SLTVPDD103 pKa = 3.5MVMPLSEE110 pKa = 4.32VIQRR114 pKa = 11.84YY115 pKa = 6.52TRR117 pKa = 11.84GQAVPTLPAVYY128 pKa = 10.44ADD130 pKa = 4.45IDD132 pKa = 3.98LSPYY136 pKa = 10.87DD137 pKa = 5.21DD138 pKa = 4.48LDD140 pKa = 3.45KK141 pKa = 11.34FQIIDD146 pKa = 3.61LQRR149 pKa = 11.84KK150 pKa = 8.24LEE152 pKa = 3.93HH153 pKa = 6.77RR154 pKa = 11.84ARR156 pKa = 11.84MLKK159 pKa = 9.42TDD161 pKa = 4.4IEE163 pKa = 4.28LTKK166 pKa = 11.16NKK168 pKa = 9.35IRR170 pKa = 11.84SQKK173 pKa = 10.21EE174 pKa = 3.45KK175 pKa = 10.06SDD177 pKa = 3.33YY178 pKa = 10.16DD179 pKa = 3.6ARR181 pKa = 11.84VAAAAKK187 pKa = 10.35ALSQTLNNDD196 pKa = 2.86NKK198 pKa = 9.35TQNSS202 pKa = 3.65

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1453

102

553

290.6

32.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.291 ± 2.308

1.101 ± 0.513

5.024 ± 0.392

3.923 ± 0.614

3.923 ± 0.965

5.988 ± 1.244

2.271 ± 0.498

5.437 ± 0.423

4.611 ± 1.341

9.566 ± 0.927

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.615 ± 0.158

5.85 ± 1.219

5.506 ± 0.986

5.919 ± 1.38

4.542 ± 1.314

7.158 ± 0.705

7.364 ± 0.655

5.299 ± 0.256

1.239 ± 0.35

3.372 ± 0.596
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski