Candidatus Phytoplasma oryzae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrXI (Rice yellow dwarf group)

Average proteome isoelectric point is 8.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 392 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139JPY7|A0A139JPY7_9MOLU Transcription termination/antitermination protein NusG OS=Candidatus Phytoplasma oryzae OX=203274 GN=nusG PE=3 SV=1
MM1 pKa = 7.61FEE3 pKa = 5.34LKK5 pKa = 9.5YY6 pKa = 9.39TDD8 pKa = 3.47LGEE11 pKa = 5.34GINEE15 pKa = 4.02CNIIEE20 pKa = 4.42WYY22 pKa = 10.48VKK24 pKa = 10.63VGDD27 pKa = 3.93KK28 pKa = 11.04VEE30 pKa = 4.41PEE32 pKa = 4.12TPLVNIEE39 pKa = 3.78NDD41 pKa = 3.29KK42 pKa = 11.34LRR44 pKa = 11.84DD45 pKa = 4.62DD46 pKa = 3.34ITSPVKK52 pKa = 10.01GTIKK56 pKa = 10.41KK57 pKa = 9.32ICFEE61 pKa = 4.18EE62 pKa = 4.81GQVVKK67 pKa = 10.7INEE70 pKa = 3.88VLVYY74 pKa = 10.3IEE76 pKa = 5.29LEE78 pKa = 3.95EE79 pKa = 4.94DD80 pKa = 3.19NN81 pKa = 4.88

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328IKL0|A0A328IKL0_9MOLU Elongation factor G OS=Candidatus Phytoplasma oryzae OX=203274 GN=fusA PE=3 SV=1
MM1 pKa = 7.8SKK3 pKa = 9.51IQYY6 pKa = 9.73FGTGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.11TSIARR18 pKa = 11.84VILTLGSGNIKK29 pKa = 10.03VNKK32 pKa = 9.41RR33 pKa = 11.84DD34 pKa = 3.46LEE36 pKa = 4.23KK37 pKa = 11.21YY38 pKa = 8.34ILSYY42 pKa = 7.52EE43 pKa = 4.26TRR45 pKa = 11.84LEE47 pKa = 4.19TIKK50 pKa = 10.43PLKK53 pKa = 9.24LTQSSEE59 pKa = 4.29KK60 pKa = 10.6YY61 pKa = 10.28DD62 pKa = 3.07ITVNVFGGGITSQAGAIRR80 pKa = 11.84LGISRR85 pKa = 11.84SLIKK89 pKa = 10.13IEE91 pKa = 3.99PQLRR95 pKa = 11.84TILKK99 pKa = 10.06KK100 pKa = 10.73AGLLTRR106 pKa = 11.84DD107 pKa = 3.47SRR109 pKa = 11.84CVEE112 pKa = 3.82RR113 pKa = 11.84KK114 pKa = 9.99KK115 pKa = 11.2YY116 pKa = 9.87GLKK119 pKa = 10.2KK120 pKa = 10.35ARR122 pKa = 11.84CAPQFSKK129 pKa = 11.03RR130 pKa = 3.53

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

392

0

392

118588

38

1369

302.5

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.015 ± 0.103

0.817 ± 0.027

4.263 ± 0.081

6.346 ± 0.13

6.98 ± 0.169

3.966 ± 0.125

1.576 ± 0.047

12.302 ± 0.135

13.002 ± 0.17

10.386 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.697 ± 0.034

8.231 ± 0.123

2.368 ± 0.061

3.795 ± 0.094

2.623 ± 0.069

6.057 ± 0.092

3.844 ± 0.085

4.051 ± 0.093

0.497 ± 0.036

4.185 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski