Amino acid dipepetide frequency for Corynebacterium phage TouchMeNot

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.227AlaAla: 16.227 ± 1.858
0.858AlaCys: 0.858 ± 0.363
6.397AlaAsp: 6.397 ± 0.812
8.582AlaGlu: 8.582 ± 1.008
2.653AlaPhe: 2.653 ± 0.422
9.596AlaGly: 9.596 ± 1.189
2.184AlaHis: 2.184 ± 0.391
5.071AlaIle: 5.071 ± 0.586
4.993AlaLys: 4.993 ± 0.607
8.894AlaLeu: 8.894 ± 0.915
3.979AlaMet: 3.979 ± 0.661
2.809AlaAsn: 2.809 ± 0.454
6.163AlaPro: 6.163 ± 0.883
7.333AlaGln: 7.333 ± 1.284
6.553AlaArg: 6.553 ± 0.873
7.333AlaSer: 7.333 ± 0.943
6.865AlaThr: 6.865 ± 0.932
7.489AlaVal: 7.489 ± 0.816
2.184AlaTrp: 2.184 ± 0.475
2.262AlaTyr: 2.262 ± 0.344
0.0AlaXaa: 0.0 ± 0.0
Cys
0.78CysAla: 0.78 ± 0.262
0.234CysCys: 0.234 ± 0.139
0.39CysAsp: 0.39 ± 0.18
0.624CysGlu: 0.624 ± 0.252
0.234CysPhe: 0.234 ± 0.143
0.858CysGly: 0.858 ± 0.328
0.078CysHis: 0.078 ± 0.096
0.312CysIle: 0.312 ± 0.169
0.468CysLys: 0.468 ± 0.204
0.624CysLeu: 0.624 ± 0.246
0.156CysMet: 0.156 ± 0.105
0.39CysAsn: 0.39 ± 0.177
0.39CysPro: 0.39 ± 0.2
0.234CysGln: 0.234 ± 0.159
0.468CysArg: 0.468 ± 0.199
0.468CysSer: 0.468 ± 0.205
0.078CysThr: 0.078 ± 0.078
0.312CysVal: 0.312 ± 0.165
0.156CysTrp: 0.156 ± 0.131
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.88AspAla: 7.88 ± 0.624
0.468AspCys: 0.468 ± 0.162
3.355AspAsp: 3.355 ± 0.558
4.681AspGlu: 4.681 ± 0.652
2.34AspPhe: 2.34 ± 0.399
4.681AspGly: 4.681 ± 0.577
1.404AspHis: 1.404 ± 0.375
3.433AspIle: 3.433 ± 0.505
2.418AspLys: 2.418 ± 0.475
6.631AspLeu: 6.631 ± 0.686
1.326AspMet: 1.326 ± 0.409
1.248AspAsn: 1.248 ± 0.224
3.589AspPro: 3.589 ± 0.496
2.575AspGln: 2.575 ± 0.485
3.979AspArg: 3.979 ± 0.682
3.043AspSer: 3.043 ± 0.458
2.965AspThr: 2.965 ± 0.471
4.759AspVal: 4.759 ± 0.649
1.248AspTrp: 1.248 ± 0.396
1.248AspTyr: 1.248 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
7.021GluAla: 7.021 ± 0.719
0.234GluCys: 0.234 ± 0.153
3.901GluAsp: 3.901 ± 0.436
4.915GluGlu: 4.915 ± 0.877
1.638GluPhe: 1.638 ± 0.331
4.759GluGly: 4.759 ± 0.758
1.794GluHis: 1.794 ± 0.421
4.759GluIle: 4.759 ± 0.735
3.511GluLys: 3.511 ± 0.596
6.319GluLeu: 6.319 ± 0.94
1.716GluMet: 1.716 ± 0.394
1.872GluAsn: 1.872 ± 0.463
3.121GluPro: 3.121 ± 0.435
3.823GluGln: 3.823 ± 0.604
4.525GluArg: 4.525 ± 0.738
3.823GluSer: 3.823 ± 0.704
2.731GluThr: 2.731 ± 0.45
5.227GluVal: 5.227 ± 0.772
0.858GluTrp: 0.858 ± 0.235
1.638GluTyr: 1.638 ± 0.36
0.0GluXaa: 0.0 ± 0.0
Phe
2.965PheAla: 2.965 ± 0.681
0.312PheCys: 0.312 ± 0.172
2.653PheAsp: 2.653 ± 0.511
1.794PheGlu: 1.794 ± 0.465
1.326PhePhe: 1.326 ± 0.294
2.34PheGly: 2.34 ± 0.474
0.39PheHis: 0.39 ± 0.206
1.638PheIle: 1.638 ± 0.321
0.936PheLys: 0.936 ± 0.318
1.716PheLeu: 1.716 ± 0.425
0.468PheMet: 0.468 ± 0.192
0.624PheAsn: 0.624 ± 0.29
1.482PhePro: 1.482 ± 0.474
0.312PheGln: 0.312 ± 0.209
1.638PheArg: 1.638 ± 0.41
2.496PheSer: 2.496 ± 0.461
2.028PheThr: 2.028 ± 0.44
1.95PheVal: 1.95 ± 0.359
0.546PheTrp: 0.546 ± 0.233
1.092PheTyr: 1.092 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
8.738GlyAla: 8.738 ± 1.128
0.234GlyCys: 0.234 ± 0.183
5.383GlyAsp: 5.383 ± 0.669
5.773GlyGlu: 5.773 ± 0.633
2.418GlyPhe: 2.418 ± 0.573
6.241GlyGly: 6.241 ± 0.981
1.716GlyHis: 1.716 ± 0.499
5.227GlyIle: 5.227 ± 0.82
3.433GlyLys: 3.433 ± 0.544
6.007GlyLeu: 6.007 ± 0.759
1.794GlyMet: 1.794 ± 0.353
2.262GlyAsn: 2.262 ± 0.376
2.965GlyPro: 2.965 ± 0.543
3.433GlyGln: 3.433 ± 0.738
4.213GlyArg: 4.213 ± 0.81
5.695GlySer: 5.695 ± 1.021
3.511GlyThr: 3.511 ± 0.746
5.617GlyVal: 5.617 ± 0.767
1.794GlyTrp: 1.794 ± 0.483
1.872GlyTyr: 1.872 ± 0.485
0.0GlyXaa: 0.0 ± 0.0
His
2.262HisAla: 2.262 ± 0.421
0.234HisCys: 0.234 ± 0.147
0.858HisAsp: 0.858 ± 0.313
1.404HisGlu: 1.404 ± 0.326
1.17HisPhe: 1.17 ± 0.319
1.248HisGly: 1.248 ± 0.313
0.78HisHis: 0.78 ± 0.21
1.404HisIle: 1.404 ± 0.253
0.468HisLys: 0.468 ± 0.219
1.248HisLeu: 1.248 ± 0.371
0.312HisMet: 0.312 ± 0.16
0.234HisAsn: 0.234 ± 0.148
1.326HisPro: 1.326 ± 0.347
1.014HisGln: 1.014 ± 0.278
1.248HisArg: 1.248 ± 0.315
1.17HisSer: 1.17 ± 0.293
1.17HisThr: 1.17 ± 0.513
1.326HisVal: 1.326 ± 0.429
0.39HisTrp: 0.39 ± 0.155
0.546HisTyr: 0.546 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
6.163IleAla: 6.163 ± 0.704
0.468IleCys: 0.468 ± 0.229
4.915IleAsp: 4.915 ± 0.668
4.525IleGlu: 4.525 ± 0.587
1.638IlePhe: 1.638 ± 0.401
5.071IleGly: 5.071 ± 0.819
1.248IleHis: 1.248 ± 0.371
2.418IleIle: 2.418 ± 0.468
2.028IleLys: 2.028 ± 0.372
2.575IleLeu: 2.575 ± 0.531
0.78IleMet: 0.78 ± 0.253
1.794IleAsn: 1.794 ± 0.313
2.731IlePro: 2.731 ± 0.512
1.716IleGln: 1.716 ± 0.336
3.277IleArg: 3.277 ± 0.641
3.901IleSer: 3.901 ± 0.534
4.213IleThr: 4.213 ± 0.609
3.199IleVal: 3.199 ± 0.493
0.858IleTrp: 0.858 ± 0.279
1.092IleTyr: 1.092 ± 0.383
0.0IleXaa: 0.0 ± 0.0
Lys
5.305LysAla: 5.305 ± 0.717
0.078LysCys: 0.078 ± 0.092
3.277LysAsp: 3.277 ± 0.531
1.872LysGlu: 1.872 ± 0.503
0.936LysPhe: 0.936 ± 0.33
2.731LysGly: 2.731 ± 0.61
1.014LysHis: 1.014 ± 0.25
2.965LysIle: 2.965 ± 0.448
2.028LysLys: 2.028 ± 0.494
4.603LysLeu: 4.603 ± 0.721
0.702LysMet: 0.702 ± 0.224
1.95LysAsn: 1.95 ± 0.276
1.716LysPro: 1.716 ± 0.406
1.248LysGln: 1.248 ± 0.401
3.199LysArg: 3.199 ± 0.506
2.418LysSer: 2.418 ± 0.47
3.355LysThr: 3.355 ± 0.6
3.043LysVal: 3.043 ± 0.672
0.312LysTrp: 0.312 ± 0.148
0.78LysTyr: 0.78 ± 0.277
0.0LysXaa: 0.0 ± 0.0
Leu
10.61LeuAla: 10.61 ± 0.847
0.78LeuCys: 0.78 ± 0.294
4.759LeuAsp: 4.759 ± 0.628
5.539LeuGlu: 5.539 ± 0.602
1.482LeuPhe: 1.482 ± 0.301
7.333LeuGly: 7.333 ± 0.941
1.326LeuHis: 1.326 ± 0.333
3.589LeuIle: 3.589 ± 0.529
3.901LeuLys: 3.901 ± 0.589
6.163LeuLeu: 6.163 ± 0.805
2.106LeuMet: 2.106 ± 0.395
2.418LeuAsn: 2.418 ± 0.447
4.759LeuPro: 4.759 ± 0.752
2.809LeuGln: 2.809 ± 0.629
5.227LeuArg: 5.227 ± 0.756
5.383LeuSer: 5.383 ± 0.754
4.525LeuThr: 4.525 ± 0.651
4.837LeuVal: 4.837 ± 0.677
1.092LeuTrp: 1.092 ± 0.341
1.404LeuTyr: 1.404 ± 0.354
0.0LeuXaa: 0.0 ± 0.0
Met
1.872MetAla: 1.872 ± 0.47
0.156MetCys: 0.156 ± 0.101
1.326MetAsp: 1.326 ± 0.411
1.17MetGlu: 1.17 ± 0.315
0.312MetPhe: 0.312 ± 0.174
2.028MetGly: 2.028 ± 0.414
0.312MetHis: 0.312 ± 0.184
1.404MetIle: 1.404 ± 0.379
1.638MetLys: 1.638 ± 0.421
2.731MetLeu: 2.731 ± 0.399
0.546MetMet: 0.546 ± 0.194
0.858MetAsn: 0.858 ± 0.261
1.482MetPro: 1.482 ± 0.369
0.936MetGln: 0.936 ± 0.299
1.404MetArg: 1.404 ± 0.33
2.262MetSer: 2.262 ± 0.435
1.794MetThr: 1.794 ± 0.446
1.014MetVal: 1.014 ± 0.378
0.078MetTrp: 0.078 ± 0.091
0.39MetTyr: 0.39 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.057AsnAla: 4.057 ± 0.566
0.156AsnCys: 0.156 ± 0.109
1.56AsnAsp: 1.56 ± 0.327
1.716AsnGlu: 1.716 ± 0.35
1.092AsnPhe: 1.092 ± 0.398
2.184AsnGly: 2.184 ± 0.562
0.468AsnHis: 0.468 ± 0.191
1.248AsnIle: 1.248 ± 0.251
1.326AsnLys: 1.326 ± 0.463
2.653AsnLeu: 2.653 ± 0.541
0.156AsnMet: 0.156 ± 0.124
0.39AsnAsn: 0.39 ± 0.16
2.184AsnPro: 2.184 ± 0.437
0.78AsnGln: 0.78 ± 0.26
1.638AsnArg: 1.638 ± 0.251
1.482AsnSer: 1.482 ± 0.322
1.17AsnThr: 1.17 ± 0.309
1.638AsnVal: 1.638 ± 0.311
0.702AsnTrp: 0.702 ± 0.266
0.624AsnTyr: 0.624 ± 0.248
0.0AsnXaa: 0.0 ± 0.0
Pro
5.071ProAla: 5.071 ± 0.604
0.078ProCys: 0.078 ± 0.075
3.277ProAsp: 3.277 ± 0.614
3.745ProGlu: 3.745 ± 0.597
2.106ProPhe: 2.106 ± 0.464
4.447ProGly: 4.447 ± 0.586
0.78ProHis: 0.78 ± 0.21
3.043ProIle: 3.043 ± 0.484
1.482ProLys: 1.482 ± 0.342
3.589ProLeu: 3.589 ± 0.725
1.014ProMet: 1.014 ± 0.229
0.858ProAsn: 0.858 ± 0.268
2.262ProPro: 2.262 ± 0.718
1.95ProGln: 1.95 ± 0.363
2.965ProArg: 2.965 ± 0.517
3.121ProSer: 3.121 ± 0.435
3.511ProThr: 3.511 ± 0.601
2.965ProVal: 2.965 ± 0.462
1.404ProTrp: 1.404 ± 0.334
1.326ProTyr: 1.326 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
5.305GlnAla: 5.305 ± 1.015
0.312GlnCys: 0.312 ± 0.245
1.95GlnAsp: 1.95 ± 0.369
3.433GlnGlu: 3.433 ± 0.556
1.638GlnPhe: 1.638 ± 0.313
2.887GlnGly: 2.887 ± 0.571
0.702GlnHis: 0.702 ± 0.248
1.95GlnIle: 1.95 ± 0.376
1.638GlnLys: 1.638 ± 0.391
3.901GlnLeu: 3.901 ± 0.624
0.702GlnMet: 0.702 ± 0.262
0.936GlnAsn: 0.936 ± 0.281
1.17GlnPro: 1.17 ± 0.301
2.028GlnGln: 2.028 ± 0.417
3.277GlnArg: 3.277 ± 0.429
2.653GlnSer: 2.653 ± 0.642
2.262GlnThr: 2.262 ± 0.455
2.965GlnVal: 2.965 ± 0.581
1.092GlnTrp: 1.092 ± 0.316
1.092GlnTyr: 1.092 ± 0.334
0.0GlnXaa: 0.0 ± 0.0
Arg
7.255ArgAla: 7.255 ± 0.622
0.78ArgCys: 0.78 ± 0.415
3.199ArgAsp: 3.199 ± 0.544
4.681ArgGlu: 4.681 ± 0.81
2.496ArgPhe: 2.496 ± 0.46
3.589ArgGly: 3.589 ± 0.747
0.858ArgHis: 0.858 ± 0.342
3.979ArgIle: 3.979 ± 0.583
3.121ArgLys: 3.121 ± 0.729
5.695ArgLeu: 5.695 ± 0.824
1.716ArgMet: 1.716 ± 0.404
2.184ArgAsn: 2.184 ± 0.49
2.575ArgPro: 2.575 ± 0.363
2.575ArgGln: 2.575 ± 0.586
6.787ArgArg: 6.787 ± 1.035
3.979ArgSer: 3.979 ± 0.621
2.653ArgThr: 2.653 ± 0.452
3.199ArgVal: 3.199 ± 0.645
1.56ArgTrp: 1.56 ± 0.391
1.56ArgTyr: 1.56 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
7.88SerAla: 7.88 ± 1.341
0.312SerCys: 0.312 ± 0.161
3.979SerAsp: 3.979 ± 0.621
3.433SerGlu: 3.433 ± 0.526
1.482SerPhe: 1.482 ± 0.346
6.007SerGly: 6.007 ± 0.983
1.248SerHis: 1.248 ± 0.391
2.809SerIle: 2.809 ± 0.396
3.199SerLys: 3.199 ± 0.517
5.071SerLeu: 5.071 ± 0.617
2.496SerMet: 2.496 ± 0.446
2.184SerAsn: 2.184 ± 0.341
2.887SerPro: 2.887 ± 0.459
2.809SerGln: 2.809 ± 0.586
4.837SerArg: 4.837 ± 0.62
5.461SerSer: 5.461 ± 0.926
3.979SerThr: 3.979 ± 0.584
4.213SerVal: 4.213 ± 0.571
1.716SerTrp: 1.716 ± 0.422
1.17SerTyr: 1.17 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
7.802ThrAla: 7.802 ± 1.042
0.234ThrCys: 0.234 ± 0.15
4.369ThrAsp: 4.369 ± 0.729
2.575ThrGlu: 2.575 ± 0.429
1.014ThrPhe: 1.014 ± 0.308
4.525ThrGly: 4.525 ± 0.7
1.17ThrHis: 1.17 ± 0.352
2.887ThrIle: 2.887 ± 0.714
2.965ThrLys: 2.965 ± 0.63
3.355ThrLeu: 3.355 ± 0.498
1.326ThrMet: 1.326 ± 0.326
1.404ThrAsn: 1.404 ± 0.4
3.277ThrPro: 3.277 ± 0.556
2.184ThrGln: 2.184 ± 0.404
3.043ThrArg: 3.043 ± 0.538
4.135ThrSer: 4.135 ± 0.925
3.667ThrThr: 3.667 ± 0.597
4.681ThrVal: 4.681 ± 0.724
1.014ThrTrp: 1.014 ± 0.267
1.248ThrTyr: 1.248 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
6.943ValAla: 6.943 ± 0.705
0.858ValCys: 0.858 ± 0.295
4.837ValAsp: 4.837 ± 0.466
5.305ValGlu: 5.305 ± 0.667
1.404ValPhe: 1.404 ± 0.373
4.135ValGly: 4.135 ± 0.598
1.326ValHis: 1.326 ± 0.266
4.369ValIle: 4.369 ± 0.636
3.121ValLys: 3.121 ± 0.518
4.681ValLeu: 4.681 ± 0.517
1.17ValMet: 1.17 ± 0.312
2.184ValAsn: 2.184 ± 0.5
2.262ValPro: 2.262 ± 0.54
2.575ValGln: 2.575 ± 0.513
3.433ValArg: 3.433 ± 0.687
5.695ValSer: 5.695 ± 0.911
3.745ValThr: 3.745 ± 0.46
5.617ValVal: 5.617 ± 0.722
1.014ValTrp: 1.014 ± 0.37
1.95ValTyr: 1.95 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
2.34TrpAla: 2.34 ± 0.509
0.078TrpCys: 0.078 ± 0.074
1.404TrpAsp: 1.404 ± 0.367
1.482TrpGlu: 1.482 ± 0.387
0.702TrpPhe: 0.702 ± 0.253
1.014TrpGly: 1.014 ± 0.364
0.234TrpHis: 0.234 ± 0.157
1.092TrpIle: 1.092 ± 0.415
0.546TrpLys: 0.546 ± 0.279
1.17TrpLeu: 1.17 ± 0.287
0.702TrpMet: 0.702 ± 0.225
0.156TrpAsn: 0.156 ± 0.116
1.17TrpPro: 1.17 ± 0.347
1.014TrpGln: 1.014 ± 0.323
0.858TrpArg: 0.858 ± 0.255
1.482TrpSer: 1.482 ± 0.39
1.404TrpThr: 1.404 ± 0.392
1.17TrpVal: 1.17 ± 0.245
0.156TrpTrp: 0.156 ± 0.114
0.312TrpTyr: 0.312 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.95TyrAla: 1.95 ± 0.4
0.39TyrCys: 0.39 ± 0.17
1.482TyrAsp: 1.482 ± 0.554
0.936TyrGlu: 0.936 ± 0.288
0.624TyrPhe: 0.624 ± 0.223
2.653TyrGly: 2.653 ± 0.441
0.78TyrHis: 0.78 ± 0.206
1.014TyrIle: 1.014 ± 0.255
0.312TyrLys: 0.312 ± 0.203
2.418TyrLeu: 2.418 ± 0.467
0.468TyrMet: 0.468 ± 0.182
0.468TyrAsn: 0.468 ± 0.211
1.482TyrPro: 1.482 ± 0.339
0.546TyrGln: 0.546 ± 0.172
1.872TyrArg: 1.872 ± 0.373
1.17TyrSer: 1.17 ± 0.309
1.248TyrThr: 1.248 ± 0.27
1.482TyrVal: 1.482 ± 0.437
0.312TyrTrp: 0.312 ± 0.165
0.78TyrTyr: 0.78 ± 0.3
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12819 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski