Alces alces faeces associated genomovirus MP43
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJA2|A0A2Z5CJA2_9VIRU Replication associated protein OS=Alces alces faeces associated genomovirus MP43 OX=2219114 PE=3 SV=1
MM1 pKa = 7.73 PFVCNARR8 pKa = 11.84 YY9 pKa = 8.93 FLVTYY14 pKa = 8.73 GHH16 pKa = 7.09 VDD18 pKa = 3.09 TLDD21 pKa = 3.61 PFTIVDD27 pKa = 3.97 FFGQLGAEE35 pKa = 4.26 VIVGRR40 pKa = 11.84 EE41 pKa = 4.0 QYY43 pKa = 10.42 HH44 pKa = 5.65 ATLGTHH50 pKa = 5.68 FHH52 pKa = 6.13 VFADD56 pKa = 4.66 FGRR59 pKa = 11.84 KK60 pKa = 8.2 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 ADD67 pKa = 3.0 IFDD70 pKa = 3.27 VDD72 pKa = 4.66 GYY74 pKa = 10.67 HH75 pKa = 7.1 PNISPSRR82 pKa = 11.84 GTPEE86 pKa = 3.98 AGYY89 pKa = 10.62 DD90 pKa = 3.63 YY91 pKa = 10.68 AIKK94 pKa = 10.92 DD95 pKa = 3.59 GDD97 pKa = 4.02 VVAGGLARR105 pKa = 11.84 PSGVGPSGRR114 pKa = 11.84 AAKK117 pKa = 8.64 WHH119 pKa = 6.38 SIIDD123 pKa = 3.51 AEE125 pKa = 4.51 TRR127 pKa = 11.84 DD128 pKa = 3.84 EE129 pKa = 5.15 FYY131 pKa = 11.19 SLCEE135 pKa = 3.81 EE136 pKa = 4.97 LDD138 pKa = 3.65 PEE140 pKa = 4.71 RR141 pKa = 11.84 LVCSFGQIQKK151 pKa = 9.8 YY152 pKa = 10.1 ADD154 pKa = 2.61 WRR156 pKa = 11.84 YY157 pKa = 9.15 RR158 pKa = 11.84 VEE160 pKa = 4.19 PEE162 pKa = 4.6 PYY164 pKa = 9.96 ASPNGVFNLAEE175 pKa = 4.28 YY176 pKa = 11.2 GDD178 pKa = 4.16 LSEE181 pKa = 4.74 SKK183 pKa = 10.8 SLILWGPSRR192 pKa = 11.84 MGKK195 pKa = 6.86 TVWARR200 pKa = 11.84 SLGNHH205 pKa = 7.1 LYY207 pKa = 10.71 FGGIFSARR215 pKa = 11.84 DD216 pKa = 3.28 INRR219 pKa = 11.84 DD220 pKa = 3.08 GVEE223 pKa = 3.73 YY224 pKa = 10.66 AIFDD228 pKa = 5.13 DD229 pKa = 3.53 IAGGIKK235 pKa = 10.03 FFPRR239 pKa = 11.84 FKK241 pKa = 10.79 DD242 pKa = 3.12 WLGCQMEE249 pKa = 4.36 FMVKK253 pKa = 9.32 EE254 pKa = 4.5 MYY256 pKa = 10.15 RR257 pKa = 11.84 DD258 pKa = 3.31 PHH260 pKa = 6.37 LFRR263 pKa = 11.84 WGRR266 pKa = 11.84 PAIWIANTDD275 pKa = 3.42 PRR277 pKa = 11.84 HH278 pKa = 6.13 DD279 pKa = 3.82 MSHH282 pKa = 6.98 EE283 pKa = 4.0 DD284 pKa = 3.94 VTWLEE289 pKa = 4.04 ANCIFVEE296 pKa = 3.75 IDD298 pKa = 3.19 SAIFHH303 pKa = 7.06 ANTEE307 pKa = 4.17
Molecular weight: 34.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.094
IPC2_protein 5.13
IPC_protein 5.105
Toseland 5.181
ProMoST 5.232
Dawson 5.181
Bjellqvist 5.296
Wikipedia 5.105
Rodwell 5.105
Grimsley 5.143
Solomon 5.181
Lehninger 5.143
Nozaki 5.334
DTASelect 5.525
Thurlkill 5.232
EMBOSS 5.207
Sillero 5.41
Patrickios 3.808
IPC_peptide 5.194
IPC2_peptide 5.41
IPC2.peptide.svr19 5.35
Protein with the highest isoelectric point:
>tr|A0A2Z5CJA2|A0A2Z5CJA2_9VIRU Replication associated protein OS=Alces alces faeces associated genomovirus MP43 OX=2219114 PE=3 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 SRR5 pKa = 11.84 YY6 pKa = 9.0 YY7 pKa = 10.96 GGGKK11 pKa = 7.6 STGWANSKK19 pKa = 9.07 RR20 pKa = 11.84 RR21 pKa = 11.84 KK22 pKa = 9.34 YY23 pKa = 9.75 RR24 pKa = 11.84 SKK26 pKa = 10.61 PRR28 pKa = 11.84 RR29 pKa = 11.84 SYY31 pKa = 10.85 AKK33 pKa = 8.96 KK34 pKa = 10.15 RR35 pKa = 11.84 SIFRR39 pKa = 11.84 SKK41 pKa = 10.79 RR42 pKa = 11.84 MSTRR46 pKa = 11.84 KK47 pKa = 9.41 ILNITATKK55 pKa = 10.5 KK56 pKa = 10.13 RR57 pKa = 11.84 DD58 pKa = 3.25 TMMAWTNSSPSSQFGGATYY77 pKa = 10.09 TNNAAVINGSAPDD90 pKa = 3.4 STAQVFLWNATARR103 pKa = 11.84 DD104 pKa = 3.62 NSTSYY109 pKa = 11.02 SAANPPTGSIFNTSTRR125 pKa = 11.84 TSSNPYY131 pKa = 8.66 MVGLAEE137 pKa = 5.19 SIEE140 pKa = 4.26 IQCNTGMPWQWRR152 pKa = 11.84 RR153 pKa = 11.84 ICFTMKK159 pKa = 10.4 GPSLVPTSTASGAVFSTSAEE179 pKa = 4.14 SSNGWLRR186 pKa = 11.84 MMNQVGGNPGQNPMYY201 pKa = 10.65 NLMVPLFRR209 pKa = 11.84 GQVGADD215 pKa = 3.26 WIDD218 pKa = 3.69 PMTAATDD225 pKa = 3.59 NSRR228 pKa = 11.84 VTIKK232 pKa = 10.5 YY233 pKa = 9.78 DD234 pKa = 3.23 KK235 pKa = 10.62 TITLSSGNEE244 pKa = 3.75 DD245 pKa = 3.51 GFIRR249 pKa = 11.84 TYY251 pKa = 10.94 KK252 pKa = 9.53 RR253 pKa = 11.84 WHH255 pKa = 6.23 PMKK258 pKa = 10.21 STLVYY263 pKa = 10.9 DD264 pKa = 4.67 DD265 pKa = 5.5 DD266 pKa = 4.23 EE267 pKa = 5.18 QGGSKK272 pKa = 8.76 TVNIQSVSGKK282 pKa = 10.59 AGMGDD287 pKa = 4.29 YY288 pKa = 11.18 YY289 pKa = 11.5 VMDD292 pKa = 4.15 MFRR295 pKa = 11.84 ARR297 pKa = 11.84 QGSAVADD304 pKa = 3.71 QLTVRR309 pKa = 11.84 PTATLYY315 pKa = 9.45 WHH317 pKa = 7.01 EE318 pKa = 4.29 KK319 pKa = 9.3
Molecular weight: 35.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.692
IPC_protein 10.101
Toseland 10.262
ProMoST 10.014
Dawson 10.467
Bjellqvist 10.16
Wikipedia 10.657
Rodwell 10.818
Grimsley 10.54
Solomon 10.496
Lehninger 10.452
Nozaki 10.248
DTASelect 10.145
Thurlkill 10.306
EMBOSS 10.672
Sillero 10.365
Patrickios 10.409
IPC_peptide 10.496
IPC2_peptide 8.931
IPC2.peptide.svr19 8.68
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
626
307
319
313.0
35.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.987 ± 0.114
1.118 ± 0.343
6.23 ± 1.284
3.994 ± 1.474
5.112 ± 1.598
8.626 ± 0.332
2.077 ± 1.011
5.112 ± 0.723
4.313 ± 0.927
4.473 ± 0.715
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.514 ± 1.047
4.633 ± 1.142
4.952 ± 0.174
2.556 ± 0.622
7.348 ± 0.122
8.626 ± 2.073
6.709 ± 2.099
5.431 ± 0.509
2.716 ± 0.074
4.473 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here