Rhodophyticola porphyridii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodophyticola

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L9Y173|A0A3L9Y173_9RHOB Integration host factor subunit alpha OS=Rhodophyticola porphyridii OX=1852017 GN=ihfA PE=3 SV=1
MM1 pKa = 7.48NFRR4 pKa = 11.84TGTFLTGAILAASALPAMAQNAEE27 pKa = 4.06LCGGVGAQGLWVGGSPEE44 pKa = 4.23ASDD47 pKa = 4.26ISTSDD52 pKa = 3.16TFIDD56 pKa = 3.59QTGLMVPPGGEE67 pKa = 4.18TVSLFSVSSAGEE79 pKa = 3.77YY80 pKa = 10.06RR81 pKa = 11.84IEE83 pKa = 4.33ANPNAGGDD91 pKa = 3.76TVIDD95 pKa = 3.53IRR97 pKa = 11.84DD98 pKa = 3.47EE99 pKa = 4.29AGTIILTDD107 pKa = 4.33DD108 pKa = 3.91DD109 pKa = 4.35SGGNFASRR117 pKa = 11.84GDD119 pKa = 3.44IALQPGTYY127 pKa = 9.57CVSTRR132 pKa = 11.84AFGGGAVVADD142 pKa = 3.86LRR144 pKa = 11.84VGRR147 pKa = 11.84AEE149 pKa = 4.62HH150 pKa = 6.39EE151 pKa = 4.12ALTPGWGGGIADD163 pKa = 4.46FAGIDD168 pKa = 3.52PCLPNTPATPLGAGAVDD185 pKa = 3.42AMLGEE190 pKa = 4.91GVTATNSIAGTPYY203 pKa = 10.54YY204 pKa = 10.1RR205 pKa = 11.84FSLSAPQALSIRR217 pKa = 11.84AEE219 pKa = 4.15NPSADD224 pKa = 3.86PYY226 pKa = 10.9IYY228 pKa = 9.86IYY230 pKa = 10.49DD231 pKa = 3.87GQGTLLAEE239 pKa = 4.31NDD241 pKa = 4.54DD242 pKa = 4.08YY243 pKa = 12.04DD244 pKa = 4.17GLNSRR249 pKa = 11.84VDD251 pKa = 3.99FTDD254 pKa = 3.86PLPAGNYY261 pKa = 8.36CIAMRR266 pKa = 11.84SLSDD270 pKa = 3.23DD271 pKa = 3.8TQPVTVSVRR280 pKa = 11.84GYY282 pKa = 10.77DD283 pKa = 3.29PAAAMQEE290 pKa = 4.02MFATGEE296 pKa = 3.9ASPPIGGSYY305 pKa = 10.16PITDD309 pKa = 4.1LGVLQTSLVMDD320 pKa = 3.31QVVGSDD326 pKa = 3.59AVWFSFSVPAGGLVLIDD343 pKa = 5.15AIEE346 pKa = 4.44ISDD349 pKa = 4.07SDD351 pKa = 3.79PVIRR355 pKa = 11.84LFDD358 pKa = 3.58GVGRR362 pKa = 11.84MIDD365 pKa = 3.75FNDD368 pKa = 4.25DD369 pKa = 3.5AGDD372 pKa = 3.86SLNSQLTVQVNPGTYY387 pKa = 8.92MLGVTQYY394 pKa = 9.73SEE396 pKa = 4.48SYY398 pKa = 10.09SGVIRR403 pKa = 11.84VALQRR408 pKa = 11.84YY409 pKa = 8.5VPAQQ413 pKa = 3.17

Molecular weight: 42.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L9Y4M4|A0A3L9Y4M4_9RHOB GNAT family N-acetyltransferase OS=Rhodophyticola porphyridii OX=1852017 GN=D9R08_09805 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 9.18RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.07SLSAA45 pKa = 3.93

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3660

0

3660

1138918

26

2388

311.2

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.589 ± 0.054

0.873 ± 0.012

6.136 ± 0.041

5.908 ± 0.039

3.722 ± 0.026

8.895 ± 0.046

2.1 ± 0.022

5.214 ± 0.03

2.523 ± 0.036

10.136 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.019

2.426 ± 0.022

5.32 ± 0.031

3.014 ± 0.02

7.138 ± 0.047

4.966 ± 0.029

5.469 ± 0.026

7.176 ± 0.033

1.411 ± 0.019

2.192 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski