candidate division MSBL1 archaeon SCGC-AAA259O05

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133V3Q7|A0A133V3Q7_9EURY SPOB_a domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA259O05 OX=1698271 GN=AKJ41_02830 PE=4 SV=1
MM1 pKa = 8.25KK2 pKa = 10.25YY3 pKa = 10.03RR4 pKa = 11.84QILILTFLIGLVAGLVPYY22 pKa = 10.94VMAVQSNNEE31 pKa = 3.9YY32 pKa = 10.86VGGFFKK38 pKa = 11.16NGGNKK43 pKa = 8.22YY44 pKa = 6.66TTDD47 pKa = 3.41KK48 pKa = 11.47AIMGNDD54 pKa = 3.19WDD56 pKa = 4.87NITEE60 pKa = 4.18GEE62 pKa = 4.25MKK64 pKa = 10.8YY65 pKa = 10.29IDD67 pKa = 3.95DD68 pKa = 3.97WVPAVLSVAGGNQQGDD84 pKa = 3.03WSGYY88 pKa = 8.05VYY90 pKa = 10.54QNPIHH95 pKa = 6.86LVRR98 pKa = 11.84DD99 pKa = 3.65DD100 pKa = 4.11SGAGGEE106 pKa = 4.32PLDD109 pKa = 6.4DD110 pKa = 3.64EE111 pKa = 5.96LFWNPQIWYY120 pKa = 9.77DD121 pKa = 5.3GNFLDD126 pKa = 5.42NKK128 pKa = 10.15GFLSTVIGTVNYY140 pKa = 6.06TTFYY144 pKa = 10.92VEE146 pKa = 4.62IRR148 pKa = 11.84GLSDD152 pKa = 4.07GYY154 pKa = 11.74ANYY157 pKa = 9.97KK158 pKa = 10.06AYY160 pKa = 10.07AYY162 pKa = 8.94DD163 pKa = 4.61TMDD166 pKa = 3.82QLINNNPYY174 pKa = 9.82VYY176 pKa = 9.46TWEE179 pKa = 3.87TDD181 pKa = 3.76YY182 pKa = 11.87YY183 pKa = 11.11SGDD186 pKa = 3.27SKK188 pKa = 11.73YY189 pKa = 10.99LVGTDD194 pKa = 3.2QGGGEE199 pKa = 4.23TLKK202 pKa = 10.63FFQFGVEE209 pKa = 4.19EE210 pKa = 4.21ASGTTWSSIGGEE222 pKa = 3.9WRR224 pKa = 11.84ISNYY228 pKa = 10.6NMGYY232 pKa = 10.14YY233 pKa = 10.33DD234 pKa = 4.63SSTNDD239 pKa = 2.46WRR241 pKa = 11.84YY242 pKa = 9.84LPGYY246 pKa = 9.12SVQHH250 pKa = 6.01EE251 pKa = 4.49DD252 pKa = 2.71SWITEE257 pKa = 4.45VEE259 pKa = 3.96GSPYY263 pKa = 10.54YY264 pKa = 10.13IGGQNYY270 pKa = 10.46DD271 pKa = 3.47GVHH274 pKa = 6.59IDD276 pKa = 3.73DD277 pKa = 4.13QGSDD281 pKa = 3.47FVMWQYY287 pKa = 11.62VEE289 pKa = 4.8GGSTVGSGVGLWSGEE304 pKa = 3.7GTYY307 pKa = 10.21IPDD310 pKa = 4.04PYY312 pKa = 10.89RR313 pKa = 5.42

Molecular weight: 35.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133V4W1|A0A133V4W1_9EURY Probable tRNA sulfurtransferase OS=candidate division MSBL1 archaeon SCGC-AAA259O05 OX=1698271 GN=thiI PE=3 SV=1
MM1 pKa = 7.43GRR3 pKa = 11.84IRR5 pKa = 11.84SHH7 pKa = 6.21AGCRR11 pKa = 11.84FSRR14 pKa = 11.84PPVVEE19 pKa = 4.34RR20 pKa = 11.84FHH22 pKa = 8.33DD23 pKa = 4.26PDD25 pKa = 4.08SYY27 pKa = 11.57GHH29 pKa = 5.74GHH31 pKa = 6.91ASFFSRR37 pKa = 11.84LAAKK41 pKa = 7.61VTKK44 pKa = 10.35RR45 pKa = 11.84IQQRR49 pKa = 11.84RR50 pKa = 11.84SKK52 pKa = 10.38ISSLRR57 pKa = 11.84GKK59 pKa = 8.35ITRR62 pKa = 11.84SLGVQDD68 pKa = 4.49DD69 pKa = 4.31HH70 pKa = 7.37AALAPKK76 pKa = 9.83RR77 pKa = 11.84DD78 pKa = 3.42LWGEE82 pKa = 3.91DD83 pKa = 4.62DD84 pKa = 6.0IPLQTPLLYY93 pKa = 10.86GRR95 pKa = 11.84VGKK98 pKa = 10.27SINEE102 pKa = 3.52IGYY105 pKa = 7.59EE106 pKa = 3.95CRR108 pKa = 11.84KK109 pKa = 9.76RR110 pKa = 11.84VSKK113 pKa = 11.03

Molecular weight: 12.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1138

0

1138

238899

48

2078

209.9

23.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.031 ± 0.083

1.064 ± 0.03

5.775 ± 0.08

10.63 ± 0.114

3.966 ± 0.058

7.696 ± 0.077

1.796 ± 0.032

6.07 ± 0.069

7.141 ± 0.095

9.075 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.032

3.508 ± 0.049

4.342 ± 0.053

2.193 ± 0.029

6.292 ± 0.082

6.564 ± 0.07

4.504 ± 0.06

6.835 ± 0.061

1.329 ± 0.042

3.014 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski