Waterbuck coronavirus US/OH-WD358/1994

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Betacoronavirus 1; Bovine coronavirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7U2N6|B7U2N6_9BETC 12.7 kDa non-structural protein OS=Waterbuck coronavirus US/OH-WD358/1994 OX=422135 PE=4 SV=1
MM1 pKa = 7.63PMATTIEE8 pKa = 4.31GADD11 pKa = 3.74YY12 pKa = 10.42TNIMPITVLTTVYY25 pKa = 10.14LGVSIGIDD33 pKa = 3.08TSTTGFTCFSRR44 pKa = 11.84YY45 pKa = 8.24

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7U2N9|B7U2N9_9BETC Nucleoprotein OS=Waterbuck coronavirus US/OH-WD358/1994 OX=422135 GN=N PE=3 SV=1
MM1 pKa = 7.55SFTPGKK7 pKa = 9.79QSSSRR12 pKa = 11.84ASSGNRR18 pKa = 11.84SGNGILKK25 pKa = 9.42WADD28 pKa = 3.13QSDD31 pKa = 3.63QSRR34 pKa = 11.84NVQTRR39 pKa = 11.84GRR41 pKa = 11.84RR42 pKa = 11.84AQPKK46 pKa = 7.96QTATSQQPPGGNVVPYY62 pKa = 8.75YY63 pKa = 10.8SWFSGITQFQKK74 pKa = 10.77GKK76 pKa = 9.42EE77 pKa = 3.95FEE79 pKa = 4.16FAEE82 pKa = 4.69GQGVPIAPGVPATEE96 pKa = 4.32AKK98 pKa = 10.35GYY100 pKa = 9.01WYY102 pKa = 10.34RR103 pKa = 11.84HH104 pKa = 4.43NRR106 pKa = 11.84RR107 pKa = 11.84SFKK110 pKa = 10.37TADD113 pKa = 3.13GNQRR117 pKa = 11.84QLLPRR122 pKa = 11.84WYY124 pKa = 9.8FYY126 pKa = 11.56YY127 pKa = 10.78LGTGPHH133 pKa = 6.97AKK135 pKa = 9.91DD136 pKa = 3.92QYY138 pKa = 10.35GTDD141 pKa = 3.18IDD143 pKa = 4.14GVFWVASNQADD154 pKa = 3.74VNTPADD160 pKa = 3.51ILDD163 pKa = 4.5RR164 pKa = 11.84DD165 pKa = 3.95PSSDD169 pKa = 3.15EE170 pKa = 4.8AIPTRR175 pKa = 11.84FPPGTVLPQGYY186 pKa = 9.38YY187 pKa = 9.56IEE189 pKa = 4.86GSGRR193 pKa = 11.84SAPNSRR199 pKa = 11.84STSRR203 pKa = 11.84ASSRR207 pKa = 11.84ASSAGSRR214 pKa = 11.84SRR216 pKa = 11.84ANSGNRR222 pKa = 11.84TPTSGVTPDD231 pKa = 3.68MADD234 pKa = 3.28QIASLVLAKK243 pKa = 10.41LGKK246 pKa = 10.07DD247 pKa = 2.95ATKK250 pKa = 9.49PQQVTKK256 pKa = 9.39QTAKK260 pKa = 10.6EE261 pKa = 3.73IRR263 pKa = 11.84QKK265 pKa = 10.67ILNKK269 pKa = 9.31PRR271 pKa = 11.84QKK273 pKa = 10.46RR274 pKa = 11.84SPNKK278 pKa = 9.02QCTVQQCFGKK288 pKa = 10.48RR289 pKa = 11.84GPNQNFGGGEE299 pKa = 3.99MLKK302 pKa = 10.67LGTSDD307 pKa = 3.38PQFPILAEE315 pKa = 4.05LAPTAGAFFFGSRR328 pKa = 11.84LEE330 pKa = 4.03LAKK333 pKa = 10.7VQNLSGNLDD342 pKa = 3.59EE343 pKa = 4.91PQKK346 pKa = 10.98DD347 pKa = 4.13VYY349 pKa = 9.8EE350 pKa = 3.97LRR352 pKa = 11.84YY353 pKa = 10.38NGAIRR358 pKa = 11.84FDD360 pKa = 3.73STLSGFEE367 pKa = 4.32TIMKK371 pKa = 9.51VLNEE375 pKa = 4.23NLNAYY380 pKa = 7.19QQQDD384 pKa = 2.72GMMNMSPKK392 pKa = 9.54PQRR395 pKa = 11.84QRR397 pKa = 11.84GQKK400 pKa = 9.76NGQGEE405 pKa = 4.35NDD407 pKa = 4.27NISVAAPKK415 pKa = 10.67SRR417 pKa = 11.84VQQNKK422 pKa = 9.07SRR424 pKa = 11.84EE425 pKa = 4.15LTAEE429 pKa = 4.92DD430 pKa = 3.26ISLLKK435 pKa = 11.03KK436 pKa = 9.46MDD438 pKa = 3.65EE439 pKa = 4.58PFTEE443 pKa = 4.44DD444 pKa = 2.7TSEE447 pKa = 3.72II448 pKa = 3.93

Molecular weight: 49.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

10124

43

4383

920.4

103.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.341 ± 0.245

3.467 ± 0.344

6.114 ± 0.27

3.645 ± 0.252

5.541 ± 0.19

6.025 ± 0.375

1.64 ± 0.189

5.383 ± 0.377

5.759 ± 0.525

8.919 ± 0.398

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.188

5.561 ± 0.657

3.516 ± 0.481

3.526 ± 0.384

3.378 ± 0.36

7.141 ± 0.304

5.966 ± 0.434

9.226 ± 0.923

1.254 ± 0.094

5.393 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski