Paracoccus isoporae
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3345 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6WDD2|A0A1G6WDD2_9RHOB N-(5'-phosphoribosyl)anthranilate isomerase OS=Paracoccus isoporae OX=591205 GN=trpF PE=3 SV=1
MM1 pKa = 8.1 RR2 pKa = 11.84 ITTGICGLLAAAMLSGGAALAQTEE26 pKa = 4.34 DD27 pKa = 3.66 AEE29 pKa = 4.48 AAASDD34 pKa = 3.81 MPEE37 pKa = 4.39 AEE39 pKa = 4.41 SVTTDD44 pKa = 2.59 SDD46 pKa = 3.77 GAAGQDD52 pKa = 3.45 ATGSMSGTDD61 pKa = 3.23 TDD63 pKa = 4.56 ADD65 pKa = 4.21 PGTMSGSDD73 pKa = 3.05 TGADD77 pKa = 3.37 SGAMSGSDD85 pKa = 3.13 TAEE88 pKa = 4.12 TEE90 pKa = 4.17 PGGAAAGMGTDD101 pKa = 3.79 AAPAHH106 pKa = 6.47 SATISDD112 pKa = 4.29 AEE114 pKa = 4.38 GAQIGAVEE122 pKa = 4.5 LSFSASGLAVVDD134 pKa = 3.92 ISFTGVPAGEE144 pKa = 4.4 HH145 pKa = 5.8 AVHH148 pKa = 6.51 FHH150 pKa = 4.32 QTGMCEE156 pKa = 4.08 APFDD160 pKa = 4.17 SAGDD164 pKa = 3.89 HH165 pKa = 6.58 LAGDD169 pKa = 3.94 MDD171 pKa = 4.83 HH172 pKa = 7.24 GVMAQNGPHH181 pKa = 7.07 PGDD184 pKa = 3.56 MPNITVPDD192 pKa = 3.64 SGEE195 pKa = 3.54 ISLTYY200 pKa = 9.08 FVPGLTEE207 pKa = 5.28 DD208 pKa = 5.12 LVADD212 pKa = 4.35 DD213 pKa = 5.64 DD214 pKa = 4.17 GTAFVIHH221 pKa = 6.49 EE222 pKa = 4.52 ASDD225 pKa = 3.95 DD226 pKa = 3.84 YY227 pKa = 11.34 RR228 pKa = 11.84 SQPSGDD234 pKa = 2.94 AGGRR238 pKa = 11.84 LGCAVVTEE246 pKa = 4.42 AAGG249 pKa = 3.24
Molecular weight: 24.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.567
IPC_protein 3.592
Toseland 3.363
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.541
Rodwell 3.414
Grimsley 3.274
Solomon 3.579
Lehninger 3.541
Nozaki 3.706
DTASelect 3.973
Thurlkill 3.427
EMBOSS 3.554
Sillero 3.719
Patrickios 1.062
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.667
Protein with the highest isoelectric point:
>tr|A0A1G7GFW0|A0A1G7GFW0_9RHOB Solute:Na+ symporter SSS family OS=Paracoccus isoporae OX=591205 GN=SAMN05421538_11336 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 TRR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.63 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.39 AGRR29 pKa = 11.84 AVLNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.7 GRR40 pKa = 11.84 KK41 pKa = 8.85 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3345
0
3345
1059547
26
4924
316.8
34.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.998 ± 0.061
0.792 ± 0.013
6.318 ± 0.038
5.847 ± 0.038
3.526 ± 0.028
9.002 ± 0.046
1.971 ± 0.024
5.208 ± 0.033
2.526 ± 0.036
10.034 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.828 ± 0.021
2.413 ± 0.026
5.293 ± 0.033
3.25 ± 0.025
7.421 ± 0.057
5.109 ± 0.028
5.064 ± 0.034
6.898 ± 0.035
1.398 ± 0.02
2.104 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here