Angelonia flower break virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2Q474|Q2Q474_9TOMB RNA-directed RNA polymerase OS=Angelonia flower break virus OX=352882 GN=p86 PE=4 SV=2
MM1 pKa = 7.68GFFRR5 pKa = 11.84DD6 pKa = 3.77VLKK9 pKa = 11.15CGAQNAIGCTLITGAVAVGIAALEE33 pKa = 3.86VRR35 pKa = 11.84AAIGAYY41 pKa = 9.76EE42 pKa = 3.92FTNYY46 pKa = 10.64ALHH49 pKa = 6.37TSIDD53 pKa = 3.87YY54 pKa = 10.58IRR56 pKa = 11.84TRR58 pKa = 11.84GEE60 pKa = 3.72SLQPVPQYY68 pKa = 10.88PSDD71 pKa = 3.71NTSEE75 pKa = 4.18AFGVDD80 pKa = 3.65LVSEE84 pKa = 4.28EE85 pKa = 4.79SNLSDD90 pKa = 4.31HH91 pKa = 7.2LDD93 pKa = 4.11TIPEE97 pKa = 4.09EE98 pKa = 4.27TDD100 pKa = 2.87GSKK103 pKa = 10.43VVRR106 pKa = 11.84PARR109 pKa = 11.84AIIRR113 pKa = 11.84RR114 pKa = 11.84HH115 pKa = 4.78ARR117 pKa = 11.84GKK119 pKa = 10.45FSYY122 pKa = 9.68CLAMAAKK129 pKa = 9.56NAFGGTPTNTKK140 pKa = 9.68ANQMSVMRR148 pKa = 11.84FMVGKK153 pKa = 10.1CGEE156 pKa = 4.17HH157 pKa = 6.69HH158 pKa = 6.65LTDD161 pKa = 3.66ASARR165 pKa = 11.84VACAEE170 pKa = 3.86AMSVCFTPDD179 pKa = 2.48ISEE182 pKa = 3.83INMFKK187 pKa = 10.78GLNSHH192 pKa = 7.08AAYY195 pKa = 10.53LRR197 pKa = 11.84TAALRR202 pKa = 11.84EE203 pKa = 4.24AKK205 pKa = 10.16RR206 pKa = 11.84VDD208 pKa = 3.47CWWYY212 pKa = 11.12NLLLHH217 pKa = 6.84PLAANAWQRR226 pKa = 11.84AWMVLNRR233 pKa = 11.84LGDD236 pKa = 3.7LEE238 pKa = 4.16AFEE241 pKa = 5.12FIKK244 pKa = 10.95

Molecular weight: 26.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2Q472|Q2Q472_9TOMB p7 OS=Angelonia flower break virus OX=352882 GN=p7 PE=4 SV=1
MM1 pKa = 7.73LSANLNLTVLSGVIGLLLLTRR22 pKa = 11.84STLNLPSIYY31 pKa = 10.52DD32 pKa = 3.82LVPFSLPRR40 pKa = 11.84LVKK43 pKa = 10.13LDD45 pKa = 3.72QIIAFAFLGLLLNLLNCSQTVISHH69 pKa = 7.09RR70 pKa = 11.84YY71 pKa = 9.48SNDD74 pKa = 2.98NSKK77 pKa = 7.84TQYY80 pKa = 9.05VTISTPSRR88 pKa = 3.37

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1504

61

760

300.8

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.777 ± 1.381

2.261 ± 0.57

4.854 ± 0.385

4.987 ± 1.075

4.721 ± 0.475

6.915 ± 0.532

1.995 ± 0.531

5.585 ± 0.726

5.12 ± 0.889

8.577 ± 1.55

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.355

5.319 ± 0.613

4.588 ± 0.488

2.926 ± 0.304

6.516 ± 0.379

6.516 ± 1.254

6.316 ± 1.122

6.981 ± 0.747

1.662 ± 0.221

2.926 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski