Salmonella phage SSU5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9Q739|J9Q739_9CAUD Uncharacterized protein OS=Salmonella phage SSU5 OX=1177632 GN=SSU5_070 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.21ILVRR6 pKa = 11.84ISASTDD12 pKa = 2.99YY13 pKa = 11.18DD14 pKa = 3.98VYY16 pKa = 10.92PLFMVKK22 pKa = 10.04CDD24 pKa = 3.55GLSDD28 pKa = 4.18EE29 pKa = 5.43EE30 pKa = 3.88IQAAIEE36 pKa = 4.17RR37 pKa = 11.84NLVEE41 pKa = 4.25YY42 pKa = 10.15TGMDD46 pKa = 3.74ADD48 pKa = 4.3SVHH51 pKa = 7.24VDD53 pKa = 3.36DD54 pKa = 7.08DD55 pKa = 4.71GVCWHH60 pKa = 6.86NGSCWYY66 pKa = 10.73VDD68 pKa = 3.09DD69 pKa = 4.41TMPVSDD75 pKa = 4.76EE76 pKa = 4.22DD77 pKa = 3.79AAHH80 pKa = 7.27LEE82 pKa = 4.4RR83 pKa = 11.84ILGISTFEE91 pKa = 3.84

Molecular weight: 10.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9Q7Z3|J9Q7Z3_9CAUD gp32 domain-containing protein OS=Salmonella phage SSU5 OX=1177632 GN=SSU5_057 PE=4 SV=1
MM1 pKa = 7.55IPYY4 pKa = 9.89ILLSFAGGVALGFSICRR21 pKa = 11.84DD22 pKa = 3.47LVRR25 pKa = 11.84QEE27 pKa = 5.29LKK29 pKa = 10.25TKK31 pKa = 7.16TLRR34 pKa = 11.84IGKK37 pKa = 8.52RR38 pKa = 11.84LYY40 pKa = 10.46RR41 pKa = 11.84VVHH44 pKa = 4.54EE45 pKa = 4.23TGVRR49 pKa = 11.84KK50 pKa = 9.8

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

130

0

130

30424

40

1526

234.0

26.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.236 ± 0.393

1.078 ± 0.092

6.11 ± 0.181

7.412 ± 0.199

3.731 ± 0.157

6.922 ± 0.195

1.834 ± 0.138

5.699 ± 0.152

6.334 ± 0.17

8.424 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.04 ± 0.114

4.178 ± 0.121

3.599 ± 0.175

3.402 ± 0.174

5.644 ± 0.185

6.478 ± 0.249

5.722 ± 0.226

7.06 ± 0.201

1.167 ± 0.082

2.929 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski