Firmicutes bacterium CAG:212

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5YC18|R5YC18_9FIRM Transcriptional regulator LacI family OS=Firmicutes bacterium CAG:212 OX=1263009 GN=BN537_01432 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 8.75TAVVFWSGTGNTEE16 pKa = 3.52EE17 pKa = 4.14MANAVLEE24 pKa = 4.26GMKK27 pKa = 10.64EE28 pKa = 3.83NGADD32 pKa = 3.41ASIYY36 pKa = 10.64SADD39 pKa = 3.53AFQAGDD45 pKa = 3.5VEE47 pKa = 5.16GYY49 pKa = 10.41DD50 pKa = 4.63AIAFGCPAMGAEE62 pKa = 4.16VLEE65 pKa = 4.38EE66 pKa = 5.72DD67 pKa = 4.06EE68 pKa = 4.55FQPLFDD74 pKa = 3.86EE75 pKa = 5.23CKK77 pKa = 10.3SALAGKK83 pKa = 9.65KK84 pKa = 9.02VALFGSYY91 pKa = 9.06GWGDD95 pKa = 4.14GEE97 pKa = 4.97WMRR100 pKa = 11.84NWEE103 pKa = 4.3DD104 pKa = 3.29DD105 pKa = 3.53CRR107 pKa = 11.84EE108 pKa = 3.96AGIEE112 pKa = 4.03LACDD116 pKa = 3.3SVVCNDD122 pKa = 4.86APDD125 pKa = 3.72EE126 pKa = 4.34DD127 pKa = 6.32AITACKK133 pKa = 10.13EE134 pKa = 3.72LGAALAA140 pKa = 4.54

Molecular weight: 14.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5XDD6|R5XDD6_9FIRM DUF3343 domain-containing protein OS=Firmicutes bacterium CAG:212 OX=1263009 GN=BN537_00931 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2078

0

2078

679161

31

2858

326.8

36.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.253 ± 0.052

1.48 ± 0.02

5.536 ± 0.044

8.015 ± 0.066

3.976 ± 0.038

6.99 ± 0.049

1.788 ± 0.025

7.837 ± 0.045

7.306 ± 0.05

8.752 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.275 ± 0.027

4.469 ± 0.033

3.141 ± 0.031

3.4 ± 0.035

3.931 ± 0.039

5.461 ± 0.045

5.38 ± 0.04

7.068 ± 0.049

0.826 ± 0.018

4.114 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski