Caulobacter sp. B11
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1996 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G4JRR1|A0A2G4JRR1_9CAUL Methyltransferase OS=Caulobacter sp. B11 OX=2048899 GN=CSW58_07895 PE=3 SV=1
MM1 pKa = 7.99 PLTVHH6 pKa = 6.26 QFPCLSDD13 pKa = 3.3 NYY15 pKa = 10.59 GFLVRR20 pKa = 11.84 DD21 pKa = 3.74 DD22 pKa = 3.95 ATGQVATIDD31 pKa = 3.98 TPDD34 pKa = 3.03 ATT36 pKa = 4.37
Molecular weight: 3.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.923
IPC_protein 3.732
Toseland 3.528
ProMoST 3.999
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.859
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.668
EMBOSS 3.846
Sillero 3.884
Patrickios 1.952
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A2G4JSL0|A0A2G4JSL0_9CAUL Uncharacterized protein OS=Caulobacter sp. B11 OX=2048899 GN=CSW58_04285 PE=4 SV=1
FF1 pKa = 6.94 NVAATLDD8 pKa = 3.85 PTLITATLIKK18 pKa = 10.35 PGMNCNVEE26 pKa = 4.17 LAPTTLSGAVVAPLSAAISRR46 pKa = 11.84 TVSPTTLTATAQAIAGAVVAVTHH69 pKa = 6.11 GAVTAAGRR77 pKa = 11.84 WRR79 pKa = 11.84 HH80 pKa = 5.87 PWPQTLAKK88 pKa = 10.5 ALGATTGASSAGVAVGGAVNATLAVTGSSASAGVGIVAASAVTLSATGIGAAVGVGLSASAGLVLGPTLLVATLGAIVGAVVARR172 pKa = 11.84 TLGATTASGAMVAPIAANATRR193 pKa = 11.84 TLGSVTGSSTASINLAAAAGITLAATSATGVVQVALAMAASPVLGATGIAATVAAALSAGAAPVLGATVVAASVAVSLASIVNRR277 pKa = 11.84 ALGATVGVGTMSAPISASVLKK298 pKa = 9.69 TLGPSLVSSTAGVNVAAAVGVTLAATSVAASVQIALAATAAPTLGATVVTGAVGVAIAAAVSRR361 pKa = 11.84 ALGATSMSSSARR373 pKa = 11.84 AAIAATSTRR382 pKa = 11.84 TLGATSGAATASAALAAMVATVQGATSAAASAGVALAAAAGIVLGGIGRR431 pKa = 11.84 TITAGAAIAAASAITLGGIGRR452 pKa = 11.84 TITAGAAIAAAVSRR466 pKa = 11.84 TLGVTAQAATASVGIAAAVSKK487 pKa = 10.99 ALGATTGAATASIALAASLAKK508 pKa = 9.83 TLGATSIAFTVRR520 pKa = 11.84 AMRR523 pKa = 11.84 RR524 pKa = 11.84 RR525 pKa = 11.84 LVMLVRR531 pKa = 11.84 SLRR534 pKa = 11.84 PP535 pKa = 3.17
Molecular weight: 48.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.368
IPC2_protein 10.789
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.179
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.901
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1996
0
1996
526181
27
1396
263.6
28.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.731 ± 0.1
0.784 ± 0.016
5.949 ± 0.055
5.223 ± 0.05
3.563 ± 0.037
9.054 ± 0.151
1.806 ± 0.028
4.51 ± 0.039
3.19 ± 0.051
10.232 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.3 ± 0.027
2.301 ± 0.038
5.447 ± 0.049
3.163 ± 0.031
7.27 ± 0.055
5.158 ± 0.04
5.184 ± 0.044
7.581 ± 0.052
1.429 ± 0.027
2.124 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here