Streptomyces hainanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4T5P6|A0A4R4T5P6_9ACTN Ankyrin repeat domain-containing protein OS=Streptomyces hainanensis OX=402648 GN=E1283_21900 PE=4 SV=1
MM1 pKa = 7.01RR2 pKa = 11.84TARR5 pKa = 11.84PKK7 pKa = 9.52AAARR11 pKa = 11.84AFAAVIAIGLIAAGCASDD29 pKa = 5.81RR30 pKa = 11.84DD31 pKa = 4.18DD32 pKa = 6.19DD33 pKa = 5.27DD34 pKa = 6.26GSGDD38 pKa = 3.44GTGPFVFGAAGDD50 pKa = 3.96PAALDD55 pKa = 3.88PSLGSDD61 pKa = 3.48GEE63 pKa = 4.55TFRR66 pKa = 11.84VTRR69 pKa = 11.84QVFEE73 pKa = 4.08TLIEE77 pKa = 4.19HH78 pKa = 6.49EE79 pKa = 4.43PGGTEE84 pKa = 3.92MVPGLAEE91 pKa = 3.81EE92 pKa = 4.46WSSNEE97 pKa = 5.07AGTEE101 pKa = 3.75WSFTLRR107 pKa = 11.84DD108 pKa = 3.29GVTFHH113 pKa = 7.62DD114 pKa = 4.34GDD116 pKa = 3.96EE117 pKa = 4.3LTGEE121 pKa = 4.46VVCQNFEE128 pKa = 3.47RR129 pKa = 11.84WYY131 pKa = 8.91NWSGTYY137 pKa = 10.46QNTTLSYY144 pKa = 8.19YY145 pKa = 7.33WQSVFGGFAEE155 pKa = 4.36NEE157 pKa = 4.39SEE159 pKa = 4.24EE160 pKa = 4.62TPPPNYY166 pKa = 10.0VGCTAPDD173 pKa = 3.36EE174 pKa = 4.18LTAVIEE180 pKa = 4.26VAEE183 pKa = 4.49PSANYY188 pKa = 9.7PGGFSLQSFALQSPTAIEE206 pKa = 4.73AMAADD211 pKa = 4.61DD212 pKa = 4.22PSGEE216 pKa = 4.38GEE218 pKa = 4.55NITFPNYY225 pKa = 8.97SQEE228 pKa = 4.07AGVVSGTGPFQISEE242 pKa = 4.27WNRR245 pKa = 11.84SNGEE249 pKa = 3.61ITLEE253 pKa = 4.5RR254 pKa = 11.84FDD256 pKa = 6.09DD257 pKa = 3.94YY258 pKa = 11.2WGDD261 pKa = 3.35AAGVEE266 pKa = 4.19EE267 pKa = 5.01LVFRR271 pKa = 11.84TIPEE275 pKa = 3.58EE276 pKa = 3.83DD277 pKa = 3.56ARR279 pKa = 11.84RR280 pKa = 11.84QALQAGDD287 pKa = 3.29IDD289 pKa = 5.46GYY291 pKa = 11.37DD292 pKa = 3.52LVAPADD298 pKa = 3.92VEE300 pKa = 4.48TLVADD305 pKa = 4.86GFQVPVRR312 pKa = 11.84GVFNLLYY319 pKa = 10.82LGITQEE325 pKa = 4.15NNPALEE331 pKa = 4.01QLEE334 pKa = 4.25VRR336 pKa = 11.84QAIAHH341 pKa = 6.04ALDD344 pKa = 3.83RR345 pKa = 11.84EE346 pKa = 4.53NIVNTQLPEE355 pKa = 4.61GGVVATQFVPDD366 pKa = 4.72TIDD369 pKa = 3.82GYY371 pKa = 11.53SDD373 pKa = 4.77DD374 pKa = 3.92VTTYY378 pKa = 10.6EE379 pKa = 4.9HH380 pKa = 7.43DD381 pKa = 4.06PEE383 pKa = 5.1LAQQLLADD391 pKa = 5.09AGAEE395 pKa = 3.84DD396 pKa = 4.25LTIDD400 pKa = 3.97FCYY403 pKa = 8.49PTEE406 pKa = 3.95VTRR409 pKa = 11.84PYY411 pKa = 9.77MPAPADD417 pKa = 3.09IYY419 pKa = 11.11EE420 pKa = 4.37LLKK423 pKa = 10.8ADD425 pKa = 4.72LEE427 pKa = 4.58AVGITVNPVALKK439 pKa = 9.74WNPDD443 pKa = 3.4YY444 pKa = 11.65TEE446 pKa = 3.91TTRR449 pKa = 11.84DD450 pKa = 3.65GGCGVYY456 pKa = 10.81LLGWTGDD463 pKa = 3.53FNDD466 pKa = 4.77AYY468 pKa = 10.95NFLGTWFADD477 pKa = 4.11YY478 pKa = 10.68SRR480 pKa = 11.84EE481 pKa = 3.93WGFEE485 pKa = 3.89NQEE488 pKa = 4.16LFDD491 pKa = 3.99MMSAASTEE499 pKa = 4.01PDD501 pKa = 3.08VATRR505 pKa = 11.84VSLYY509 pKa = 10.45QGLNEE514 pKa = 4.72YY515 pKa = 10.56IMDD518 pKa = 4.32YY519 pKa = 10.93LPGVPISSSPPSIAFSPDD537 pKa = 2.95VNPPTVSPLTQEE549 pKa = 3.9NFAEE553 pKa = 4.69VTFKK557 pKa = 11.22

Molecular weight: 60.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4T9F4|A0A4R4T9F4_9ACTN GAF domain-containing protein OS=Streptomyces hainanensis OX=402648 GN=E1283_22705 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.43GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7007

0

7007

2299493

18

3673

328.2

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.076 ± 0.05

0.725 ± 0.007

6.117 ± 0.025

5.862 ± 0.029

2.714 ± 0.017

9.642 ± 0.027

2.296 ± 0.015

2.981 ± 0.021

1.217 ± 0.016

10.73 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.608 ± 0.013

1.704 ± 0.018

6.272 ± 0.026

2.506 ± 0.018

8.736 ± 0.029

4.738 ± 0.021

6.043 ± 0.025

8.505 ± 0.026

1.555 ± 0.013

1.974 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski