Amphiplicatus metriothermophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Parvularculales; Parvularculaceae; Amphiplicatus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239PJP6|A0A239PJP6_9PROT Uncharacterized protein OS=Amphiplicatus metriothermophilus OX=1519374 GN=SAMN06297382_0527 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84IKK4 pKa = 10.78SLGPSLFIAVMAGSTAAKK22 pKa = 10.53AEE24 pKa = 4.28DD25 pKa = 4.03LSISTTVAFEE35 pKa = 3.67SRR37 pKa = 11.84YY38 pKa = 8.25MFRR41 pKa = 11.84GVQFAEE47 pKa = 4.67TSFQPAVNLGYY58 pKa = 11.01GGFYY62 pKa = 10.53LSAWLNLPVGDD73 pKa = 5.17DD74 pKa = 4.19DD75 pKa = 6.12LVVTPGGEE83 pKa = 4.0EE84 pKa = 3.94LDD86 pKa = 4.09LVFGYY91 pKa = 10.32SADD94 pKa = 3.6LNEE97 pKa = 4.43FVSVDD102 pKa = 3.38VGVTYY107 pKa = 8.58YY108 pKa = 9.84TFPDD112 pKa = 3.61LMSGFFDD119 pKa = 4.01TFDD122 pKa = 3.95EE123 pKa = 4.54EE124 pKa = 5.53DD125 pKa = 3.45GTGANTVEE133 pKa = 4.65PYY135 pKa = 10.63VGLSFALPLSPSVYY149 pKa = 10.22LYY151 pKa = 10.82RR152 pKa = 11.84DD153 pKa = 3.39FMFDD157 pKa = 2.84TFTVQGSASHH167 pKa = 6.55SFPLSEE173 pKa = 4.97KK174 pKa = 10.8LSFDD178 pKa = 3.36LGGVLGYY185 pKa = 10.78VVDD188 pKa = 5.4DD189 pKa = 5.13DD190 pKa = 5.97DD191 pKa = 6.92GGDD194 pKa = 3.65YY195 pKa = 10.92LYY197 pKa = 11.66GHH199 pKa = 7.24ATADD203 pKa = 3.17LSYY206 pKa = 10.88AVSEE210 pKa = 4.39KK211 pKa = 10.95SSIYY215 pKa = 10.46LGARR219 pKa = 11.84YY220 pKa = 9.55GGSDD224 pKa = 3.07IDD226 pKa = 4.05GGSVIDD232 pKa = 5.11DD233 pKa = 4.41SIAGTFKK240 pKa = 11.06SSGFWWGLGFSSDD253 pKa = 3.66FF254 pKa = 3.79

Molecular weight: 27.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239PUM9|A0A239PUM9_9PROT Cytochrome c biogenesis protein CcmG thiol:disulfide interchange protein DsbE OS=Amphiplicatus metriothermophilus OX=1519374 GN=SAMN06297382_1786 PE=3 SV=1
MM1 pKa = 7.25AVKK4 pKa = 10.46RR5 pKa = 11.84KK6 pKa = 7.28ATKK9 pKa = 10.28RR10 pKa = 11.84KK11 pKa = 6.0TAKK14 pKa = 10.27KK15 pKa = 7.58ATKK18 pKa = 10.09RR19 pKa = 11.84KK20 pKa = 9.44AATKK24 pKa = 10.14KK25 pKa = 8.04ATKK28 pKa = 10.27RR29 pKa = 11.84KK30 pKa = 6.01TAKK33 pKa = 10.21KK34 pKa = 7.45ATKK37 pKa = 10.18RR38 pKa = 11.84KK39 pKa = 6.25TAKK42 pKa = 10.06KK43 pKa = 8.49AAKK46 pKa = 9.95RR47 pKa = 11.84KK48 pKa = 6.62ATKK51 pKa = 10.26RR52 pKa = 11.84KK53 pKa = 6.94TAAKK57 pKa = 8.57KK58 pKa = 7.3TAKK61 pKa = 10.03KK62 pKa = 9.23AAKK65 pKa = 9.92RR66 pKa = 11.84KK67 pKa = 4.99TAKK70 pKa = 10.26RR71 pKa = 11.84KK72 pKa = 4.98TAKK75 pKa = 10.15RR76 pKa = 11.84RR77 pKa = 11.84TKK79 pKa = 10.29KK80 pKa = 10.47AAA82 pKa = 3.35

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2934

0

2934

946893

25

2003

322.7

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.241 ± 0.094

0.815 ± 0.012

5.742 ± 0.031

6.605 ± 0.038

3.92 ± 0.036

8.61 ± 0.042

1.714 ± 0.019

4.622 ± 0.027

3.11 ± 0.037

9.783 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.019

2.247 ± 0.025

5.385 ± 0.03

2.398 ± 0.021

8.269 ± 0.045

4.516 ± 0.03

4.322 ± 0.03

6.993 ± 0.036

1.291 ± 0.017

2.339 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski