Kribbella soli
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8271 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R0HRP9|A0A4R0HRP9_9ACTN TIGR03086 family protein OS=Kribbella soli OX=1124743 GN=E0H45_04165 PE=4 SV=1
MM1 pKa = 7.75 SDD3 pKa = 3.41 QIRR6 pKa = 11.84 LEE8 pKa = 4.6 GITVNAPDD16 pKa = 4.66 ALVLAQFYY24 pKa = 11.45 AEE26 pKa = 3.86 ITGGVVSGTSHH37 pKa = 6.58 WASVSGANGVIAFQQVEE54 pKa = 4.6 DD55 pKa = 4.73 FRR57 pKa = 11.84 PPQWPGNDD65 pKa = 3.04 VPMQMHH71 pKa = 7.45 LDD73 pKa = 3.81 FLVDD77 pKa = 3.87 DD78 pKa = 5.2 LEE80 pKa = 4.54 ATGARR85 pKa = 11.84 VLAAGATLLDD95 pKa = 3.85 FQPNSDD101 pKa = 3.37 HH102 pKa = 6.99 CYY104 pKa = 10.55 VYY106 pKa = 10.93 ADD108 pKa = 3.82 PAGHH112 pKa = 7.09 PFCLSTWTTEE122 pKa = 3.63 TATAGTSSS130 pKa = 3.18
Molecular weight: 13.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 4.037
IPC_protein 3.986
Toseland 3.783
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.694
Solomon 3.973
Lehninger 3.923
Nozaki 4.113
DTASelect 4.342
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.113
Patrickios 0.896
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.978
Protein with the highest isoelectric point:
>tr|A0A4R0HK32|A0A4R0HK32_9ACTN Foldase YidC OS=Kribbella soli OX=1124743 GN=yidC PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 HH14 pKa = 5.34 KK15 pKa = 10.47 KK16 pKa = 8.84 HH17 pKa = 5.5 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIIAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.21 GRR40 pKa = 11.84 QRR42 pKa = 11.84 LAAA45 pKa = 4.56
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8271
0
8271
2671487
29
4074
323.0
34.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.528 ± 0.028
0.686 ± 0.007
6.146 ± 0.022
5.33 ± 0.028
2.98 ± 0.016
8.88 ± 0.024
2.103 ± 0.012
3.93 ± 0.02
2.427 ± 0.021
10.438 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.747 ± 0.01
2.185 ± 0.016
5.691 ± 0.021
3.151 ± 0.017
7.181 ± 0.029
5.431 ± 0.022
6.386 ± 0.027
8.805 ± 0.027
1.659 ± 0.012
2.314 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here