Actinomadura sp. GC306

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4SII8|A0A4R4SII8_9ACTN Uma2 family endonuclease (Fragment) OS=Actinomadura sp. GC306 OX=2530367 GN=E1200_22590 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84NEE5 pKa = 3.88HH6 pKa = 6.98DD7 pKa = 3.46GRR9 pKa = 11.84SLEE12 pKa = 4.02EE13 pKa = 4.0RR14 pKa = 11.84LASLDD19 pKa = 3.62AMSGEE24 pKa = 4.54PAAQSPAPAAEE35 pKa = 4.56GEE37 pKa = 4.38QGAGQPPAEE46 pKa = 4.52QEE48 pKa = 3.98NAPPEE53 pKa = 3.94EE54 pKa = 4.61DD55 pKa = 3.35APSRR59 pKa = 11.84AEE61 pKa = 3.87QTDD64 pKa = 3.78PNANPWLAAPPSFQQPPPEE83 pKa = 4.24TGYY86 pKa = 10.01PPQTFGPPQPPPYY99 pKa = 10.0EE100 pKa = 4.67GGPLPQPFDD109 pKa = 3.7GGMLPPPYY117 pKa = 10.27AGGYY121 pKa = 8.47PGQDD125 pKa = 3.48PAQAPPPYY133 pKa = 10.19VPQQSPYY140 pKa = 10.41DD141 pKa = 4.09ANGDD145 pKa = 3.76TAPPLDD151 pKa = 3.57

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4SM99|A0A4R4SM99_9ACTN Uncharacterized protein OS=Actinomadura sp. GC306 OX=2530367 GN=E1200_19345 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILTNRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.55GRR40 pKa = 11.84ARR42 pKa = 11.84IAVV45 pKa = 3.5

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6472

0

6472

2052183

26

4255

317.1

34.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.539 ± 0.052

0.797 ± 0.008

6.144 ± 0.025

5.981 ± 0.025

2.794 ± 0.017

9.67 ± 0.028

2.195 ± 0.014

3.472 ± 0.022

2.053 ± 0.025

10.209 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.011

1.738 ± 0.016

6.225 ± 0.026

2.477 ± 0.017

8.621 ± 0.035

4.608 ± 0.02

5.578 ± 0.022

8.479 ± 0.027

1.473 ± 0.011

2.033 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski